VIOLIN: Vaccine Investigation and Online Information Network

IroN

General Information

Protegen ID

282

Sequence Strain (Species/Organism)

Escherichia coli

VO ID

VO_0010988

Taxonomy ID

562

Molecule Role

Protective antigen

Molecule Role Annotation

Active immunization of BALB/c mice with IroN antigen in Freund's adjuvant protects mice from lethal challenge with ExPEC strain S26 (Durant et al., 2007).

References

Durant et al., 2007: Durant L, Metais A, Soulama-Mouze C, Genevard JM, Nassif X, Escaich S. Identification of candidates for a subunit vaccine against extraintestinal pathogenic Escherichia coli. Infection and immunity. 2007 Apr; 75(4); 1916-25. [PubMed: 17145948 ].

Gene Information

Gene Name

IroN

NCBI Gene ID

7379404

Genbank Accession

AF449498

Locus Tag

MM1_0014

Plasmid No

pAPEC-1

Gene Starting Position

9647

Gene Ending Position

11824

DNA Sequence

>NC_011980.1:9647-11824 Escherichia coli chi7122 plasmid pAPEC-1, complete sequence
ATCAGAATGATGCGGTAACTCCGGCATAGTAAGCCCGGCCTGGCTCGTTATAGGTATTCGCCCCTTCAGA
AGATCGGAAGATCTGTTTATTGAGGATATTACTGACGCCGACATTAAGACGCAGATTTTTATTAATATCG
TAATTGAAGTTCGTCCCCACCAGTGAATAAGCGCCCAGCTCTTTACCTGACAGACCGCCAGTATCTTCAC
TGCGGGTTTCCGCATGAGTACGCGGTTTTTGTCTGCCATATAACGTCCAGTTGACGCTGGCAGAAAACGC
CTGGGTGATGGTCCAGTTAAGCGAGTTATTGATAGTATATTTCGGGATGACCGACAGAGGATTACCGGTG
TCTTTTTGCTCCGAAGTGATCATCCATGTGGCATTGGTATTCCAGTTCAGACGATCTTTCACCAGTGGGA
AAGACATACTGGCTTCGATACCGTCCACCAGAGCTTTCCCGCCATTCTGCCACTTGAGGATATATGCGCC
TGAAGCGGTTTGCCCGATAACGTTATCCCCGGCCACGATCTTATTCTGGTAATCATTGCGGAAGTAGGTC
ACACTTGCGTGGTAATCTTCCCAGGTGAACTCCAGCCCAATTTCTTTATTGACGCTGATTTCCGGATCGA
GATCTTTATTACCGATCAGGTAGCACCCGCCTGATGTAATATCTTTTGGACAGCCATTGCCTTTCGAGTA
GAGCAGATAGCCTTCACTGGATTGATACAGGTTTGGGGCTTTAAAGGTTCGGGCAACCCCTGCTTTGACT
TTGAAATAATCGCCCAATTCCTGCGAAAGATTCAGACTGGGGCTGAAGTTCCCGCCGGAGTCGCTGAGAT
AATCAAAGCGCAGGCCGGGAATGATATTCGTGCCAGGAACCGGCTCAATGTTATCTTCAATATACAGCGC
ACTGATTTGAGAATGATTTTTACTGCTGCGATCCGCAGCAGAGCCAGAAATACCGCTGATATCACTGTCA
TTCACCGTCAGGCTGGTAGAGGAAGGATCATCGAGCTTATCGCGGTTCCACTCTGCACCAACGGTCAGCG
TTTGATCAACCATCACATTCAAAGGAATATTAAGCTCGCCGCTGGTTCGCCAGGAACTCAGGCGATTGGT
CGTAAACTTTTCACCCGCTAAAATACGCCCTTCACCACCGCCGGATAATCCTTCATTCATGCGGGTATTA
TTGGTTTTCTCGTAATAAACACCAAAGCGACTTTGTCCCCAGTCCCAGATACCATTATGCGTAATGCCAT
AATTCTGTCGGTACAGGCGGTTCGTCTCTTTGCCGGATTTTGCCAGGCTTTCGGTAACTGCACTGGAAGA
ACTGTTTTGCGTATCGCCCGCATAGATATTCCCCTGGCGGCTATATCCGGCTTCGAAATCGAGAATCTGC
TGCGGATTTAATTTCCACGAGACAACGCCGTTAATATCTTTGTTACGTACCCCTTCATGCCCGGCTGCGT
TTTTCGTACCGACCGGAGAATTAATATCCCAACTGTCAGCATCCGTTTTATTCAGGTTACCATACAAACG
CGTGGTAAGAGCATTACCAGCCAGAGGCCCACTAAGGCTGAAATTGGCGCGACGCGTAGCGCCCTCATCG
CTACTTTCCGGCTGATTGGTGTATAACGACAGCGAACCGTGCCAGTCGTTGGTGGGACGTTTGGTAATGA
TGTTCACCACCCCCCCGGCTGCCCCCGAACCGTAGCGCGCCGCCGCAGGGCCGCGGATCACTTCAATACG
CTCAACCTGTTCCGGTGGCACCCAGTTGGTGTCACCGCGGGTATCACGCTCTCCACGCCAGCTATAACGC
ACGGAGTTACGTGACGTCACCGGTACACCATCAATTAAAATTAAGGTGTTTTCCGGCCCCATACCACGAA
TATCGATCTGGCGGTTGTTACCGCGTGTGCCCGAGGCGCTATTGCCGGTAAGATTGACGCCAGGCATTTT
ACGAATAATATCTGAAAGGTCGTTTACCGGAGGGGTCTTTTTAATATCCTCGCTGGTAATAACCGACACG
CCCGGCTGCTGTTTTAATACCTGCTCAGCGGTGGCTTCCACCACCAGAGTCTCGTCATTATCATCGTCGG
AGGATTTGGCTACTGATACCTGGCTATTCAACCCAACCAGGAGCACAGTTAGCGACCAGAGGATTTTGTT
AATTCTCA

Protein Information

Protein Name

outer membrane receptor FepA

NCBI Protein GI

222104801

Protein Accession

YP_002539290

3D structure: PDB ID

1FEP

Protein pI

5.85

Protein Weight

74767.07

Protein Length

725

Protein Note

Fep; Cbt; Cbr; FeuB; FepA; PfeA; IroN; BfeA; outer membrane receptor of ferric enterobactin and colicins B and D; interacts with the TonB-ExbBD complex which catalyzes the translocation of the siderophore to the periplasmic space

Protein Sequence

>YP_002539290.1 IroN, siderophore receptor (plasmid) [Escherichia coli chi7122]
MRINKILWSLTVLLVGLNSQVSVAKSSDDDNDETLVVEATAEQVLKQQPGVSVITSEDIKKTPPVNDLSD
IIRKMPGVNLTGNSASGTRGNNRQIDIRGMGPENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPE
QVERIEVIRGPAAARYGSGAAGGVVNIITKRPTNDWHGSLSLYTNQPESSDEGATRRANFSLSGPLAGNA
LTTRLYGNLNKTDADSWDINSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIYAGDT
QNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRMNEGLSGGGEGRILAGE
KFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDPSSTSLTVNDSDISGISGSAADRSSKNHS
QISALYIEDNIEPVPGTNIIPGLRFDYLSDSGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEG
YLLYSKGNGCPKDITSGGCYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQ
TASGAYILKWQNGGKALVDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTGNPLSVIPKYTINNSLNWT
ITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDINKNLRLNVGVSNILNKQI
FRSSEGANTYNEPGRAYYAGVTASF

Epitope Information

IEDB Linear Epitope