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C4424

General Information
Protegen ID 284
Sequence Strain (Species/Organism) Escherichia coli CFT073
VO ID VO_0010990
Taxonomy ID 199310
Molecule Role Protective antigen
Molecule Role Annotation Active immunization of BALB/c mice with C4424 antigen in Freund's adjuvant protects mice from lethal challenge with ExPEC strain S26 (Durant et al., 2007).
COG COG5295UW, under U: Intracellular trafficking, secretion, and vesicular transport; W: Extracellular structures
Related Vaccines(s) E. coli C4424 protein vaccine
References
Durant et al., 2007: Durant L, Metais A, Soulama-Mouze C, Genevard JM, Nassif X, Escaich S. Identification of candidates for a subunit vaccine against extraintestinal pathogenic Escherichia coli. Infection and immunity. 2007; 75(4); 1916-1925. [PubMed: 17145948].
Gene Information
Gene Name C4424
NCBI Gene ID 1038067
Genbank Accession AE014075
Locus Tag c4424
Gene Starting Position 4205983
Gene Ending Position 4211319
DNA Sequence 
>NC_004431.1:4205983-4211319 Escherichia coli CFT073, complete genome
AATGAACAAAATATTTAAAGTTATCTGGAATCCGGCAACAGGCAGTTACACCGTTGCCAGCGAAACGGCG
AAGAGCCGTGGTAAAAAAAGCGGGCGCAGTAAGCTGTTAATTTCTGCACTGGTTGCGGGTGGGTTGTTGT
CGTCGTTTGGGGCAAGTGCAGATAATTACACTGGGCAGCCAACTGATTATGGCGATGGCTCAGCAGGTGA
CGGCTGGGTTGCTATCGGTAAAGGGGCAAAAGCAAATACCTTTATGAACACTAGTGGCGCGAGTACAGCT
TTAGGATATGACGCGATAGCCGAAGGTGAGTACAGTTCTGCCATCGGGTCAAAAACCCTTGCAACTGGTG
GAGCATCCATGGCGTTCGGGGTTAGTGCAAAAGCAATGGGTGACAGAAGTGTCGCGCTAGGTGCATCGTC
AGTAGCAAATGGCGATCGTTCGATGGCTTTTGGTCGTTACGCAAAGACGAATGGTTTTACATCTCTTGCT
ATTGGGGACTCCTCCCTTGCCGATGGTGAAAAAACTATTGCGTTAGGAAATACGGCTAAAGCTTACGAAA
TTATGAGCATCGCCCTCGGTGATAATGCCAATGCGTCAAAAGAGTATGCAATGGCGCTGGGAGCAAGTAG
CAAAGCTGGCGGTGCTGATAGCCTCGCATTCGGCAGAAAATCTACAGCTAATAGCACTGGCTCACTGGCA
ATAGGTGCTGACAGTAGCAGTTCGAACGATAACGCCATCGCGATAGGGAACAAAACGCAAGCCCTGGGAG
TGAATTCGATGGCCCTGGGTAATGCAAGTCAGGCATCTGGCGAATCCAGTATTGCATTAGGTAACACCAG
TGAAGCCAGCGAACAAAATGCGATTGCGCTGGGGCAAGGTAGCATTGCAAGCAAAGTGAACTCAATCGCG
TTGGGAAGTAACAGTTTGTCCTCGGGAGAGAATGCCATCGCATTGGGAGAGGGTAGTGCCGCTGGTGGCA
GCAACAGCCTTGCTTTCGGTAGCCAGTCCAGGGCAAACGGCAATGATTCTGTCGCCATCGGTGTAGGGGC
TGCAGCAGCGACCGACAATTCTGTCGCTATCGGCGCAGGATCGACCACAGATGCAAGCAATACGGTTTCA
GTTGGCAACAGCGCAACAAAACGCAAAATTGTTAATATGGCTGCTGGTGCCATAAGCAACACCAGTACCG
ATGCCATCAACGGCTCACAGCTTTATACGATCAGTGATTCAGTCGCCAAGCGACTCGGAGGAGGCGCTAC
TGTAGGCAGCGATGGCACCGTAACCGCAGTAAGCTACGCGTTGAGAAGCGGAACCTATAATAACGTGGGT
GATGCTCTGTCAGGAATCGACAATAATACCCTACAATGGAATAAAACCGCGGGGGCGTTCAGCGCCAATC
ACGGTGCAAATGCCACCAACAAAATCACTAATGTTGCTAAAGGTACGGTTTCTGCAACCAGCACCGATGT
AGTAAACGGCTCTCAATTGTACGACCTGCAGCAGGATGCTCTGTTGTGGAACGGCACAGCATTCAGTGCC
GCACACGGCACCGAAGCCACCAGCAAAATCACTAACGTCACCGCTGGCAACCTGACTGCCGGCAGCACTG
ACGCCGTTAACGGCTCTCAGCTCAAAACCACCAACGACAACGTGACGACCAACACCACCAACATCGCCAC
TAACACCACCAATATCACCAACCTGACTGACGCTGTTAACGGTCTCGGTGACGACTCCCTGCTGTGGAAC
AAAGCAGCTGGCGCATTCAGCGCCGCGCACGGCACCGAAGCCACCAGCAAAATCACCAACGTCACCGCTG
GCAACCTGACTGCCGGTAGCACTGACGCCGTTAACGGCTCCCAGCTCAAAACCACCAACGACAACGTGAC
GACCAACACCACCAACATCGCCACTAACACCACCAATATCACCAACCTGACTGACGCTGTTAACGGTCTC
GGTGACGACTCCCTGCTGTGGAACAAAACAGCTGGCGCATTCAGCGCCGCGCACGGCACTGACGCCACCA
GCAAGATCACCAACGTCACCGCTGGCAACCTGACTGCCGGCAGCACTGACGCCGTTAACGGCTCCCAGCT
CAAAACCACCAACGACAACGTGACGACCAACACCACCAACATCGCCACTAACACCACCAATATCACCAAC
CTGACTGACGCTGTTAACGGTCTCGGTGACGACTCCCTGCTGTGGAACAAAACAGCTGGCGCATTCAGCG
CCGCGCACGGCACTGACGCCACCAGCAAGATCACCAATGTCAAAGCCGGTGACCTGACAGCTGGCAGCAC
TGACGCCGTTAACGGCTCTCAGCTCAAAACCACCAACGATAACGTGTCGACCAACACCACCAACATCACC
AACCTGACTGACGCTGTTAACGGTCTCGGTGACGACTCCCTGCTGTGGAACAAAACAGCTGGCGCATTCA
GCGCCGCTCACGGCACTGACGCCACCAGCAAGATCACCAATGTCAAAGCCGGTGACCTGACAGCTGGCAG
CACTGACGCCGTTAACGGCTCCCAGCTCAAAACCACCAACGATAACGTGTCGACCAACACCACCAACATC
ACTAACCTGACGGATTCCGTTGGCGACCTTAAGGACGATTCTCTGCTGTGGAACAAAGCGGCTGGCGCAT
TCAGCGCCGCGCACGGTACCGAAGCTACCAGCAAGATCACCAACTTACTGGCTGGCAAGATATCTTCTAA
CAGCACTGATGCCATTAATGGCTCACAACTTTATGGCGTAGCGGATTCATTTACGTCATATCTTGGTGGT
GGTGCTGATATCAGCGATACGGGTGTATTAAGTGGGCCAACCTACACTATTGGTGGTACTGACTACACTA
ACGTCGGTGATGCTCTGGCAGCCATTAACACATCATTTAGCACATCACTCGGCGACGCCCTACTTTGGGA
TGCAACCGCAGGCAAATTCAGCGCCAAACACGGCATTAATAATGCTCCCAGTGTAATCACTGATGTTGCA
AACGGTGCAGTCTCGTCCACCAGCAGCGACGCCATTAACGGTTCACAACTTTATGGTGTTAGTGACTACA
TTGCCGATGCTCTGGGCGGGAATGCTGTGGTGAACACTGACGGCAGTATCACTACACCAACTTATGCCAT
CGCTGGCGGCAGTTACAACAACGTCGGTGACGCGCTGGAAGCGATCGATACCACGCTGGATGATGCTCTG
CTGTGGGATACAACAGCCAATGGCGGTAACGGTGCATTTAGCGCCGCTCACGGGAAAGATAAAACTGCCA
GTGTAATCACTAACGTCGCTAACGGTGCAGTCTCTGCCACCAGCAACGATGCCATTAATGGCTCACAGCT
CTATAGCACTAATAAGTACATCGCTGATGCGCTGGGTGGTGATGCAGAAGTCAACGCTGACGGTACTATC
ACTGCACCGACTTACACCATTGCAAATACCGATTACAACAACGTCGGTGAAGCCCTGGATGCGCTCGATA
ATAACGCGCTGCTGTGGGATGAAGACGCAGGTGCCTACAACGCCAGCCATGATGGCAATGCCAGCAAAAT
CACCAACGTTGCGGCTGGTGATCTCTCCACAACCAGTACCGATGCTGTTAACGGTTCCCAGTTAAACGCA
ACCAATATTCTGGTTACGCAAAATAGCCAAATGATTAACCAGCTTGCTGGTAACACTAGCGAAACCTACA
TCGAGGAAAACGGTGCGGGTATTAACTATGTACGTACCAACGACAGCGGCTTAGCGTTCAACGATGCCAG
CGCTTCAGGTATTGGCGCTACAGCTGTAGGTTATAACGCAGTTGCCTCTCATGCCAGCAGTGTAGCCATC
GGTCAGGACAGCATCAGCGAAGTTGATACGGGTATCGCTCTGGGTAGCAGTTCCGTTTCCAGCCGTGTAA
TAGTTAAAGGGACTCGTAACACCAGCGTATCGGAAGAAGGTGTTGTGATTGGTTATGACACCACGGATGG
CGAACTGCTTGGCGCGTTGTCGATTGGTGATGACGGTAAATATCGTCAAATCATCAACGTCGCGGATGGT
TCTGAAGCCCATGATGCGGTCACTGTTCGCCAGTTGCAAAACGCCATTGGTGCAGTCGCAACCACACCAA
CCAAATACTATCACGCCAACTCAACGGCTGAAGACTCACTGGCAGTCGGTGAAGACTCGCTGGCAATGGG
CGCGAAAACCATCGTTAATGGTAATGCGGGTATTGGTATCGGCCTGAACACGCTGGTTCTGGCTGATGCG
ATCAACGGTATTGCTATCGGTTCTAACGCACGCGCAAATCATGCCGACAGCATTGCAATGGGTAATGGTT
CTCAGACTACCCGTGGTGCGCAGACCAACTACACTGCCTACAACATGGATGCACCGCAGAACTCTGTGGG
TGAGTTCTCTGTCGGCAGTGAAGACGGTCAACGTCAGATCACCAACGTCGCAGCAGGTTCGGCGGATACC
GATGCGGTTAACGTGGGTCAGTTGAAAGTAACGGACGCGCAGGTTTCCCAGAATACCCAGAGCATTACTA
ACCTGAACACTCAGGTCACTAATCTGGATACTCGCGTGACCAATATCGAAAACGGCATTGGCGATATCGT
AACCACCGGTAGCACTAAGTACTTCAAGACCAACACCGATGGCGCAGATGCCAACGCGCAGGGTAAAGAC
AGTGTTGCGATTGGTTCTGGTTCCATTGCTGCCGCTGACAACAGCGTCGCACTGGGCACGGGTTCCGTAG
CAGACGAAGAAAACACCATCTCTGTGGGTTCTTCTACCAACCAGCGTCGTATCACCAACGTTGCTGCCGG
TGTTAATGCCACCGATGCGGTTAACGTTTCGCAACTGAAGTCTTCTGAAGCAGGCGGCGTTCGCTACGAC
ACCAAAGCTGATGGCTCTATCGACTACAGCAACATCACTCTCGGTGGCGGCAATAGCGGTACGACTCGCA
TCAGCAACGTTTCTGCTGGCGTGAACAACAACGACGCAGTGAACTATGCGCAGTTGAAGCAAAGTGTGCA
GGAAACGAAGCAATACACCGATCAGCGCATGGTTGAGATGGATAACAAACTGTCCAAAACTGAAAGCAAG
CTGAGTGGTGGTATCGCTTCTGCAATGGCAATGACCGGTCTGCCGCAGGCTTACACGCCGGGTGCCAGCA
TGGCCTCTATTGGTGGCGGTACTTACAACGGTGAATCGGCTGTTGCTTTAGGTGTGTCGATGGTGAGCGC
CAATGGTCGTTGGGTCTACAAATTACAAGGTAGTACCAATAGCCAGGGTGAATACTCCGCCGCACTCGGT
GCCGGTATTCAGTGGTA
Protein Information
Protein Name adhesin
NCBI Protein GI 26250246
Protein Accession NP_756286
Protein pI 4.25
Protein Weight 166959.01
Protein Length 1778
Protein Note Escherichia coli O157:H7 ortholog: z5029
Protein Sequence 
>NP_756286.1 adhesin [Escherichia coli CFT073]
MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVAGGLLSSFGASADNYTGQPTDYGDGSAGD
GWVAIGKGAKANTFMNTSGASTALGYDAIAEGEYSSAIGSKTLATGGASMAFGVSAKAMGDRSVALGASS
VANGDRSMAFGRYAKTNGFTSLAIGDSSLADGEKTIALGNTAKAYEIMSIALGDNANASKEYAMALGASS
KAGGADSLAFGRKSTANSTGSLAIGADSSSSNDNAIAIGNKTQALGVNSMALGNASQASGESSIALGNTS
EASEQNAIALGQGSIASKVNSIALGSNSLSSGENAIALGEGSAAGGSNSLAFGSQSRANGNDSVAIGVGA
AAATDNSVAIGAGSTTDASNTVSVGNSATKRKIVNMAAGAISNTSTDAINGSQLYTISDSVAKRLGGGAT
VGSDGTVTAVSYALRSGTYNNVGDALSGIDNNTLQWNKTAGAFSANHGANATNKITNVAKGTVSATSTDV
VNGSQLYDLQQDALLWNGTAFSAAHGTEATSKITNVTAGNLTAGSTDAVNGSQLKTTNDNVTTNTTNIAT
NTTNITNLTDAVNGLGDDSLLWNKAAGAFSAAHGTEATSKITNVTAGNLTAGSTDAVNGSQLKTTNDNVT
TNTTNIATNTTNITNLTDAVNGLGDDSLLWNKTAGAFSAAHGTDATSKITNVTAGNLTAGSTDAVNGSQL
KTTNDNVTTNTTNIATNTTNITNLTDAVNGLGDDSLLWNKTAGAFSAAHGTDATSKITNVKAGDLTAGST
DAVNGSQLKTTNDNVSTNTTNITNLTDAVNGLGDDSLLWNKTAGAFSAAHGTDATSKITNVKAGDLTAGS
TDAVNGSQLKTTNDNVSTNTTNITNLTDSVGDLKDDSLLWNKAAGAFSAAHGTEATSKITNLLAGKISSN
STDAINGSQLYGVADSFTSYLGGGADISDTGVLSGPTYTIGGTDYTNVGDALAAINTSFSTSLGDALLWD
ATAGKFSAKHGINNAPSVITDVANGAVSSTSSDAINGSQLYGVSDYIADALGGNAVVNTDGSITTPTYAI
AGGSYNNVGDALEAIDTTLDDALLWDTTANGGNGAFSAAHGKDKTASVITNVANGAVSATSNDAINGSQL
YSTNKYIADALGGDAEVNADGTITAPTYTIANTDYNNVGEALDALDNNALLWDEDAGAYNASHDGNASKI
TNVAAGDLSTTSTDAVNGSQLNATNILVTQNSQMINQLAGNTSETYIEENGAGINYVRTNDSGLAFNDAS
ASGIGATAVGYNAVASHASSVAIGQDSISEVDTGIALGSSSVSSRVIVKGTRNTSVSEEGVVIGYDTTDG
ELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYYHANSTAEDSLAVGEDSLAMG
AKTIVNGNAGIGIGLNTLVLADAINGIAIGSNARANHADSIAMGNGSQTTRGAQTNYTAYNMDAPQNSVG
EFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIV
TTGSTKYFKTNTDGADANAQGKDSVAIGSGSIAAADNSVALGTGSVADEENTISVGSSTNQRRITNVAAG
VNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNSGTTRISNVSAGVNNNDAVNYAQLKQSVQ
ETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSA
NGRWVYKLQGSTNSQGEYSAALGAGIQW
Vaxign Prediction
Localization(Probability) Outer Membrane
(Prob.=0.995)
Adhesin Probability 0.939
Trans-membrane Helices 0
Detailed Vaxign Results Vaxign Results
Epitope Information
IEDB Linear Epitope