VIOLIN: Vaccine Investigation and Online Information Network

ompA

General Information

Protegen ID

285

Sequence Strain (Species/Organism)

Escherichia coli CFT073

VO ID

VO_0010991

Taxonomy ID

562

Other Database IDs

CDD:225439
CDD:143586

Molecule Role

Protective antigen

Molecule Role Annotation

Active immunization of BALB/c mice with recombinant E. coli protein C3389 antigen in Freund's adjuvant protects mice from lethal challenge with ExPEC strain S26 (Durant et al., 2007).

recombinant antigens (rAg) vaccine combining common ExPEC surface proteins EtsC, OmpA, OmpT, and TraT showed significantly (P < 0.05) elicited IgY against specific antigens, induced immune related mRNA expression in the spleen and bursa, reduced in vitro growth of multiple APEC serotypes, and decreased bacterial loads in the heart and spleen, and gross lesion scores of the air sac, heart and liver in chickens. (Van et al., 2017)

COG

COG2885M, under M: Cell wall/membrane/envelope biogenesis

Related Vaccines(s)

E. coli C3389 protein vaccine

References

Durant et al., 2007: Durant L, Metais A, Soulama-Mouze C, Genevard JM, Nassif X, Escaich S. Identification of candidates for a subunit vaccine against extraintestinal pathogenic Escherichia coli. Infection and immunity. 2007; 75(4); 1916-1925. [PubMed: 17145948].

Van et al., 2017: Van Goor A, Stromberg ZR, Mellata M. A recombinant multi-antigen vaccine with broad protection potential against avian pathogenic Escherichia coli. PloS one. 2017; 12(8); e0183929. [PubMed: 28837660].

Gene Information

Gene Name

ompA

NCBI Gene ID

1039252

Genbank Accession

AE014075

Locus Tag

c3389

Gene Starting Position

3227151

Gene Ending Position

3228803

DNA Sequence

>NC_004431.1:3227151-3228803 Escherichia coli CFT073, complete genome
AGTGAGGAACACGCTGAAACAGGCCATCGTGCTGTGGGGAATGGTGTTACTGCTGGTGCTGTGGTCAGTG
TTTATCAGTCCGTCTGGCGTGCTGAGATGGGCCGGTGCGGCGGCTATCGTTCTGGCGGTTGCCGCGTTGT
TGATTTATCGGCGCAGGCAGGCGTGGACGGAGATGACCGGCGATGCCGGGTTGTCATCGCTGCCGCCGGA
AACCTACCGACAGCCGGTAGTGCTGGTCTGTGGCGGTCTGTCGGCGCACCTGTCCACTGACAGCCCGGTC
CGCCAGGTTTCAGAAGGGCTGTATCTGCATGTTCCTGATGAAGAACAGCTTGTGGCGCAGGTGGAGCGAT
TGCTGACCCTTCGCCCGGCGTGGGCATCGCAGCTTGCCGTGGCGTATACCATCATGCCCGGCATACACCG
GGATGTGGCGGTTCTGGCCGGACGGCTGCGACGGTTCGCCCACAGTATGGCGACGGTGCGTCGTCGGGCA
GGCGTAAACGTCCCCTGGCTTCTCTGGAGCGGGCTGTCCGGCTCGCCGTTGCCGGAAAGAGCGAGTTCAC
CGTGGTTTATCTGTACCGGCGGCGAAGTTCAGGTAGCAACATCCACAGAGACCACCATGCCCGCGCAGTG
GATTGCACAATCCGGCGTACAGGAGCGCAGTCAGCGACTCTGTTACCTGCTGAAAGCTGAAAGCCTGATG
CAGTGGCTGAATCTTAATGTGCTGACGGCACTGAACGGCCCGGAGGCGAAATGTCCACCACTGGCGATGA
CCGTGGGGCTGGTCCCCTCGTTGCCTGCGGTGGATAACAACCTGTGGCAGTTGTGGATCACCGCCAGAAC
CGGCCTGACGCCGGATATCGCGGACACCGGCACAGACGATGCGCTGCCATTCCCGGATGCCCTGTTACGG
CAGTTGCCGCGTCAGTCGGGCTTTACCCCGCTGCGACGAGCCTGCGTGACCATGCTGGGCGTCACCACCG
TGGCGGGTATCGCCGCGCTGTGCCTGTCAGCCACGGCAAATCGCCAGTTATTACGGCAGGTCGGTGACGA
TCTGCACCGGTTTTATGCCGTCCCGGTGGAGGAATTTATCACCAAAGCCCGTCACCTGTCGGTGCTGAAA
GACGATGCGACCATGCTCGATGGGTATTACCGGGAAGGAGAACCCCTGCGCCTCGGTCTGGGGTTATACC
CCGGCGAACGCATCCGCCAGCCGGTATTACGCGCCATTCGCGACTGGCGTCCGCCTGAACAAAAAATGGA
GGTGACGGCTTCGCTTCAGGTTCAGACCGTGCGTCTTGACAGTATGTCGCTGTTTGACGTCGGACAGGCC
CGCCTGAAAGACGGCTCGACAAAAGTGCTGGTGGACGCACTGGTGAACATCCGGGCAAAACCGGGCTGGC
TGATCCTCGTGGCCGGATATACCGATGCCACCGGCGATGAAAAAAGCAATCAGCAGTTATCGCTGCGGCG
TGCCGAAGCGGTGCGCAACTGGATGCTGCAGACCAGCGACATCCCGGCCACCTGTTTTGCCGTACAGGGA
CTGGGCGAGAGCCAGCCTGCGGCGACCAACGACACGCCACAGGGCCGGGCAGTCAACCGGCGTGTCGAAA
TCAGTCTTGTTCCGCGTTCTGACGCCTGTCAGGACGTGAAATA

Protein Information

Protein Name

OmpA family protein

NCBI Protein GI

WP_001246526

Protein Accession

NP_755264

Protein pI

8.72

Protein Weight

55874.8

Protein Length

614

Protein Note

Outer membrane protein OmpA and related peptidoglycan-associated (lipo)proteins [Cell wall/membrane/envelope biogenesis]; COG2885

Protein Sequence

>WP_001246526.1 OmpA family protein [Escherichia coli]
MRNTLKQAIVLWGMVLLLVLWSVFISPSGVLRWAGAAAIVLAVAALLIYRRRQAWTEMTGDAGLSSLPPE
TYRQPVVLVCGGLSAHLSTDSPVRQVSEGLYLHVPDEEQLVAQVERLLTLRPAWASQLAVAYTIMPGIHR
DVAVLAGRLRRFAHSMATVRRRAGVNVPWLLWSGLSGSPLPERASSPWFICTGGEVQVATSTETTMPAQW
IAQSGVQERSQRLCYLLKAESLMQWLNLNVLTALNGPEAKCPPLAMTVGLVPSLPAVDNNLWQLWITART
GLTPDIADTGTDDALPFPDALLRQLPRQSGFTPLRRACVTMLGVTTVAGIAALCLSATANRQLLRQVGDD
LHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLGLGLYPGERIRQPVLRAIRDWRPPEQKME
VTASLQVQTVRLDSMSLFDVGQARLKDGSTKVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRR
AEAVRNWMLQTSDIPATCFAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK

Epitope Information

IEDB Linear Epitope