VIOLIN: Vaccine Investigation and Online Information Network

H2-K1

General Information

Protegen ID

292

Sequence Strain (Species/Organism)

Mus musculus

Taxonomy ID

10090

Molecule Role

Protective antigen

Molecule Role Annotation

A mouse H-2Kb (H2-K1) gene was introduced into two poorly immunogenic tumor cell lines, a mouse colonic carcinoma cell line, MCA-26 (H-2Kd), and a rat mammalian carcinoma cell line, LN-4, in an effort to stimulate tumor rejection. Results showed that the expression of xenogeneic MHC class I antigen completely abolished the LN-4 tumorigenicity in rats, whereas the expression of allogeneic MHC class I antigen only partially reduced the MCA-26 tumorigenicity in mice. The immunized rats that experienced LN-4/H-2Kb tumor regression further developed protective immunity against a subsequent challenge of LN-4 cells. Adenovirus-mediated H-2Kb gene transfer in vivo can further significantly inhibit pre-established LN-4 tumors (Campbell et al., 2000).

Related Vaccines(s)

Cancer recombinant vector vaccine encoding H-2Kb , Poliovirus vector expressing model antigen H2-Kb-restricted CTL epitope

References

Campbell et al., 2000: Campbell I, Moyana T, Carlsen S, Zheng C, Xiang J. Adenoviral transfer of xenogeneic MHC class I gene results in loss of tumorigenicity and inhibition of tumor growth. Cancer gene therapy. 2000; 7(1); 37-44. [PubMed: 10678354].

Gene Information

Gene Name

H2-K1

NCBI Gene ID

14972

Genbank Accession

AB114280

Chromosome No

Gene Starting Position

33996011

Gene Ending Position

34000340

DNA Sequence

>NC_000083.6:33996011-34000340 Mus musculus strain C57BL/6J chromosome 17, GRCm38.p4 C57BL/6J
ATAGTGACCCAGATTCTGGAAGTTTATTCATCTATCATTTATTTCTTCAAAAAAACAAATCAATCAAACA
AACAAACAAAACCTCTAAGCATTCTCAATCCATGAAATTAACAAGAAATCAGCCCTAGGTCAAGATGATA
ACAATCAAGGTTACATTCAAATTATTATTCTCAGTGTGGAAGTGAGGAGTTGCAGGTCAGGGTAGCATGG
AGCTGACAGGCTACAGATGTCCCTCAGTGTTTGGCTGAACCAGCAAGGTTGGCTGTGGAAGGGAAGACAG
AGCAGTGCAGGGACAGGGTCCTGGTGTAGAGGGGTGGACTGGGCTCCACAGTTCTTCACATTGAGTCCAT
AGGCTCACAGGGAACATCAGACACTTGACTAAAGAGAACTGAGGGCTCTGGATGTCACAGGAGATATGAG
AAGACATTGTCTGTCACCAAGTCCACTCCAGGCAGCTGTCTTCACGCTAGAGAATGAGGGTCATGAACCA
TCACTGTGAAGACAACATTCGAACAACCCATCACTCACTCACAGGGGATTCTGGGAGTTCCTGAAACCCA
ATCATGTCCACATTGCCCCTCCCCAATCAGACCCTAGAGTGTCACCTTTACAATCTGGGAGAGACAGATC
AGAGGTCTGGGAGCCTAGGGTAAAACAAAACAAATGTATATGCATATGTACATGTACATATTCATTTCAT
GGCACAACTCAGGCACCTAGAAGATTTCTCTGGAGGAGCTATTATGGATTCCCAGAGCTCCCATCATCTC
CAACTTCACTCCAATGTCCCCAGGACAACTCTGTCCCCACACTAACCTGGAGCCAGAGCATAGTCCCCTC
CTTTTCCACCTGTGAGAAGAAAAGCTGTGAGAGTTCCAGGAAGATCTTGACACTAGGAAGTTCCCAGAAC
TGACAGCAGACCCAGTTAGAGACAGTAGCAATGTGGGGTGTGCCTGTGTTCCCCATTGATGAGCAGAGCA
CACTCTAAAGGAGGCTGAGAGAAAACTCAGACTCTGCCCTTTCCTACCTGTGTTTCTCCTTCTCATCTTC
ATCACAAAAGCCACCACAGCTCCAGTGACTATTGCAGCTCCAAGGACAACCAGAACAGCAACGGTCGCCA
TGTTGGAGACAGTGGATGGAGGAGGCTCTGGGAAGGACAGGTACAAGAAATGAAGGTGAGGGTCATGACC
CCAGCTCTCAGCCCTGAGCAGCTCAGGGTCTGCAGAAAGCTCCAGCTTTCCCTGACCCCAGCTCTGCACC
CACACTCTCCTTACCCCATCTCAGGGTGAGGGGCTCAGGCAGCCCCTGATGGTACACATGGCATGTGTAA
TACTGCTCCTTCCCAAGAGGCACCACCACAGATGCCCACTTCTGGAAGGTTCCATCCCCTGCAGGCCTGG
TCTCCACAAGCTCCATGTCCTGGATCAGCTCCTCCCCATTCAACTGCCAGGTCAGGGTGATGTCAGCAGG
GTAGAAGCCCAGGGCCCAGCACCTCAGGGTGACTTTATCTTCAGGTCTGCTGTGATGGGTCACATGGGCC
TTTGGGGAATCTGTGTGGAAGATTTAAGAAATCACACAATTAACAAGACAAACTGAAATCTACCCAAGAC
TCAGTTAAATCAATGATGACAGTGAACTATGTGTCTGTTTGACACTTCAACTGTCATCGCACTAGTGACC
AAAAGCAGAGCTGGTCCCCTCAGCACAGGACTGCACATTTTCTTTTGGGACTCAGAAGCTAAGAGGGAAT
CCACATGAGCCATTGGGTAAACATGGCCATCCTTATGTGATGTTCAATAGCATGAGAGAGGTAGTCAAAG
AATGGAGGAGGGAATGGAAAATAACTAATTTAATATGTGTTTGAGTTCATATTAAAATTAGGGCTGTATG
ATCCTGGAAGGTGACACCATTGAGACTCAACCATTGTAGTCACCTTGCTGACCAACATTAACTGTCAGTC
CAAGTGAACAGCCATCACTAAGTGTGTAGCCAGAGAAAATGACTCCCAGCCCCTGACTCTGGGGGACAAG
GTACAGGTGTGTCACACAGGATCCCTGGAGTGTGCTGTGGACCCCAGTGCAGCACTCACTGGAACACAGT
GGGCTCACAGGGTGTGTGGTCTTCCCTCTCACTCAGCCCTGGATTGCAGAAGACCCTGCCTCTCAGAGTC
CAAGTCCTGATTTACTAGCGGGAGGGAGCACTGTAACTTGTGGGGCAGAGGAAGAGCAGGAGGCTTCTGC
ATGTTTAGTTCTGACAGGAAACAGTCTAAGTTCACAGGAATCTCTTCTTTAATTCGAACCCTGATTTAAC
TTGTCTAATTATCCAGGTTAGCAGCCTTCCCTGGCACCTGTGGAGACTGAAACAAATGATAATAGGAGGG
CATGGAGAGACTGGATCACTCCAATGCAGACAAAAGCTGACTAGAAACAAAATAAGCCCCCCCCCCCACC
AAAAAAAAAGTTAAGAAAAAAGAAAGGCCAGGAGTGGTGGCACATGCCTTTAATCCCAGCACTCGGGAGC
CAGAGGCAGGCAGATTTCTGAGTTCAAGGCCAACCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCT
ATACAGAGAAACCCCGTCTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGGAAAGAGA
GAGAGAGAGAGAGAGAGAAGAAAATCCACGATTTATATTCCTCCAGGGTAGCCCATAAGGGCTCATGTGA
CCATTCACCACTGTAGGAATAGATAGAATTGGGTTGTTAAGGGGTGCAAAACCCCACAGACAGGGAGGGA
TCTGTGAGAATGTATTCTTTGGAAAATTCTAGAAACTAGGTGAACCGTCCTAGTGTAGAGGAGTCCATGT
CTCTTATCAGGTAAGGAGACTTCCGGTCCTGAGGTGTAAGGGCTGACACATTCAGCAGGACAGGAGTCAG
TGTCTACAGACAGTGGGCGTGGGCAGAGAACCCGGCCTGGGAGAGGCCATGGCTGACAGAGGCTGCAGGG
GAACTGAAGGGAGCAGCTGTTGTTACCTCAGGGAAATTCTCTCCTTCCCTCCTGAGACAGCCTGGGCACT
GTCCAGGGAGAAGGCTGAGCCCTGGGAGAGTCAGTGCAGTCACTGTGATGAGGGATCAGGAGACCAGCGA
CACTCTCTCCCCTCTGAGACAGGGGCATCACCCCAGCTGAGGGTTTCTTCTTCCCCAGGACTGAGCCCCA
GCCCGAGGGCAGAGGGAGGAGCTGCCCGCGGCCCCTGCACCTGTGCGCAGCAGCGTCGCGTTCCCGTTCT
TCAGGTATCTGCGGAGCCACTCCACGCACGTGCCCTCCAGGTAGGCCCTGAGTCTCTCTGCTTCACCAGC
CTGCTCCCACTTGTGTTTGGTGATCAGCGCCGCCATGTCCGCCGCCGTCCACGTTTTCAGGTCTTCGTTC
AGGGCGATGTAATCGCAGCCGTCGTAGGCGTACTGCTGGTACCCGCGGAGGAGTCGCCCGTCGGACCCCA
CTTCACAGCCAGAGATCACCTGAATAGTGTGAGAGCCTGCGGGACCCCGCGGTCAGCCCCGCTCACCCGC
CCCGGCCCCGCCCGCCCGCGGACTCCTCCAAACTGAAAGAGAAACCGGTTCCGGGTCCGTTCTGTTCCCG
AACCTCGGACTTGGGACCCGGGACGTCAGCGTCCCTGTGTCGGGACGTGGAGGGGTCGTGACCTCCGACC
CGGGGTCACTCACCGCCCTTGCTCTGGTTGTAGTAGCCGAGCAGGGTCCTCAGGTCCACTCGGAAACTCT
GCTCATTGCCCTTGGCTTTCTGTGTCTCCCGCTCCCAATACTCGGGCCCCTCCTGCTCCATCCACCGCGC
CCGCGGCTCATATCTCGGATTCTCCGCGTCGCTGTCGAAGCGCACGAACTCCGTGTCGTCCACGTAGCCG
ACTTCCATGTACCGGGGCTCCCCGAGGCCGGGCCGGGACACGGCGGTGACGAAATACCTCAGCGAGTGTG
GGCCTGGGGGCGGCGCGCGGTGAGACCCGGACCACCTCACCAGACCCCGGGCGGCTGCGCGGGCTGAGAG
GGGAGCAGGGCGCGGGACTCGGGTGGAGAAGGGGCGGAGGGTCCGGTGGGCGACCCGAGGACGCGGCTTC
CCCGGTGCCGCCCCTCCCCTCAGAGGCCGTTTCCCTCCCGGCCCGGTACTCACCCGCGCGGGTCTGAGTC
GGAGCCAGGGCGGCCGCCAACAGCAGGAGCAGCGTGCACGGTACCATCGCACCTGTCGGCGATTCGCGAC
TTCTGAGTTCTGCGGGCTGCGTGGACTTTATATCCAGCGTCCGCGGCGACACTGATTGGT

Protein Information

Protein Name

transcript variant X11

NCBI Protein GI

133922588

Protein Accession

NP_001001892

Protein pI

6.35

Protein Weight

38523.57

Protein Length

369

Protein Note

Also known as K-f; H-2K; H2-K; H-2K(d); MGC7052; MGC184092

Protein Sequence

>NP_001001892.2 H-2 class I histocompatibility antigen, K-W28 alpha chain isoform 1 precursor [Mus musculus]
MVPCTLLLLLAAALAPTQTRAGPHSLRYFVTAVSRPGLGEPRYMEVGYVDDTEFVRFDSDAENPRYEPRA
RWMEQEGPEYWERETQKAKGNEQSFRVDLRTLLGYYNQSKGGSHTIQVISGCEVGSDGRLLRGYQQYAYD
GCDYIALNEDLKTWTAADMAALITKHKWEQAGEAERLRAYLEGTCVEWLRRYLKNGNATLLRTDSPKAHV
THHSRPEDKVTLRCWALGFYPADITLTWQLNGEELIQDMELVETRPAGDGTFQKWASVVVPLGKEQYYTC
HVYHQGLPEPLTLRWEPPPSTVSNMATVAVLVVLGAAIVTGAVVAFVMKMRRRNTGGKGGDYALAPGSQT
SDLSLPDCKVMVHDPHSLA

Epitope Information

IEDB Linear Epitope