VIOLIN: Vaccine Investigation and Online Information Network

US6

General Information

Protegen ID

390

Sequence Strain (Species/Organism)

Bovine alphaherpesvirus 1

VO ID

VO_0011041

Taxonomy ID

10320

Molecule Role

Protective antigen

Molecule Role Annotation

Calves were injected intranasally with either AdCMVgC or AdCMVgD (US6) or a combination of these two recombinants or a commercially available live vaccine for comparison. The highest BHV-1 neutralizing antibody titres were obtained with AdCMVgD (US6) followed by the live vaccine and to a lower extent with the combination of the two recombinants. Calves were protected against intranasal BHV-1 challenge performed 3 weeks after the second immunization (Gogev et al., 2002).

Related Vaccines(s)

Bovine herpesvirus 1 DNA vaccine AdCMVgD , Bovine herpesvirus 1 DNA vaccine tgD-VP22 , Bovine herpesvirus 1 recombinant vector vaccine rLaSota/gDF encoding gD , Bovine herpesvirus 1 recombinant vector vaccine rLaSota/gDFL encoding gD

References

Gogev et al., 2002: Gogev S, Vanderheijden N, Lemaire M, Schynts F, D'Offay J, Deprez I, Adam M, Eloit M, Thiry E. Induction of protective immunity to bovine herpesvirus type 1 in cattle by intranasal administration of replication-defective human adenovirus type 5 expressing glycoprotein gC or gD. Vaccine. 2002; 20(9-10); 1451-1465. [PubMed: 11818166].

Gene Information

Gene Name

US6

NCBI Gene ID

1487406

Genbank Accession

KU198480

Locus Tag

BoHV1_gp41

Gene Starting Position

118895

Gene Ending Position

121435

DNA Sequence

>NC_001847.1:118895-121435 Bovine herpesvirus 1, complete genome
CATGCAAGGGCCGACATTGGCCGTGCTGGGCGCGCTGCTCGCCGTTGCGGTGAGCTTGCCTACACCCGCG
CCGCGGGTGACGGTATACGTCGACCCGCCGGCGTACCCGATGCCGCGATACAACTACACTGAACGCTGGC
ACACTACCGGGCCCATACCGTCGCCCTTCGCAGACGGCCGCGAGCAGCCCGTCGAGGTGCGCTACGCGAC
GAGCGCGGCGGCGTGCGACATGCTGGCGCTGATCGCAGACCCGCAGGTGGGGCGCACGCTGTGGGAAGCG
GTACGCCGGCACGCGCGCGCGTACAACGCCACGGTCATATGGTACAAGATCGAGAGCGGGTGCGCCCGGC
CGCTGTACTACATGGAGTACACCGAGTGCGAGCCCAGGAAGCACTTTGGGTACTGCCGCTACCGCACACC
CCCGTTTTGGGACAGCTTCCTGGCGGGCTTCGCCTACCCCACGGACGACGAGCTGGGACTGATTATGGCG
GCGCCCGCGCGGCTCGTCGAGGGCCAGTACCGACGCGCGCTGTACATCGACGGCACGGTCGCCTATACAG
ATTTCATGGTTTCGCTGCCGGCCGGGGACTGCTGGTTCTCGAAACTCGGCGCGGCTCGCGGGTACACCTT
TGGCGCGTGCTTCCCGGCCCGGGATTACGAGCAAAAGAAGGTTCTGCGCCTGACGTATCTCACGCAGTAC
TACCCGCAGGAGGCACACAAGGCCATAGTCGACTACTGGTTCATGCGCCACGGGGGCGTCGTTCCGCCGT
ATTTTGAGGAGTCGAAGGGCTACGAGCCGCCGCCTGCCGCCGATGGGGGTTCCCCCGCGCCACCCGGCGA
CGACGAGGCCCGCGAGGATGAAGGGGAGACCGAGGACGGGGCAGCCGGGCGGGAGGGCAACGGCGGCCCC
CCAGGACCCGAAGGCGACGGCGAGAGTCAGACCCCCGAAGCCAACGGAGGCGCCGAGGGCGAGCCGAAAC
CCGGCCCCAGCCCCGACGCCGACCGCCCCGAAGGCTGGCCGAGCCTCGAAGCCATCACGCACCCCCCGCC
CGCCCCCGCTACGCCCGCGGCCCCCGACGCCGTGCCGGTCAGCGTCGGGATCGGCATTGCGGCTGCGGCG
ATCGCGTGCGTGGCCGCCGCCGCCGCCGGCGCGTACTTCGTCTATACGCGCCGGCGCGGTGCGGGTCCGC
TGCCCAGAAAGCCAAAAAAGCTGCCGGCCTTTGGCAACGTCAACTACAGCGCGCTGCCCGGGTGAGCGGC
CTAGGCCCTCCCCCGACCGCCCCCTTTGCTCCTAGCCCCGGCTCCTGCCGAGCCGCGCGGGGCGGGAGAT
AAAGCGCCCGCGCGTCGGCGACTCAAGCCATTGCCGCGACCTTGTCCTCCGGCGCGCTCGCGATGCGGTG
CCTGTTGCTCTGGATGGTGGTGCTGGCCGCGCGAGCGGCGCCCGCTCGCAGCCTTGTGTATCGCGGCGAG
GCAGTCGGCCTGCGCGCGGACGGCCCCGTAGCGTTCGCTGTCCACCCGACCGACGCAACGCTCGCGCTGC
GGGGCCGGCTGATTTTCCTGGAACACCAGCTCCCGGCCGGGCGGCGCTACAACGGGACCGTCGAGCTGCT
GCGCTACCACGCCGCGGGCGACTGCTTCGTTATGCTGCAGACGACCGCGTTCGCCTCCTGCCCGCGCGTC
GCGAACGACGCCTTTCGCTCCTGCCTGCACGCCGACACGCGCCCCGCTCGCAGCGAGCGGCGCGCGAGCG
CCGCGGTCGAAAACCACGTGCTCTTCTCCATCGCCCATCCGCGCCCAATAGACTCAGGGCTCTACTTTCT
GCGCGTCGGCATCTACGGCGGCACCGCGGGCAGCGAGCGCCGCCGAGACGTCTTTCCCTTGGCCGCGTTT
GTACACAGCTTCGGTGAGCCCGGAGACCCAGAGGCCGCGGCCGCGCACCCCGGCGCCGTCGAGGCAGTCG
CGACCCGCTGCGAGCGGGGCCTCGACGCCAGCTCGGCGAGCCTCTACGACCGCGCGCTGGCGGCGTTCCC
CGCAGGCGCCGCCACCACGCCCGGCCCCACCGCGAGCAGCGGGAGCGGGGCCGCGACGCCGGAGAGGGTC
GACGAGACGACGGAAGTCAAGGCCGCGACGAGAGCGGGCTCGGCGTTTGCCCTCACCACGCCCCCGGCCG
GCCTGACCGCCAGCCCCGCCGCCAGCCCCTCCCGTGCCTTTAGCGCGGCCGCCCCGGCCGCCGCTGCGCA
GCCGGCCGGAGACACGCCCGCTCGCTTCCGGCGCCAACTGGCGTCGATCCTAGTGCCTCTGTGCGTGCTG
GTGCTGCTGCTGCTTGCGCTCTGCGCCGCGACGGTAAACTGCGCGCTGCGCCGCCGCCTGCTGCCGTGCT
CTCGGCGCGTTTACAAGCCGCGGACGTGCGCGACATGCGGGAGCGGCACTTGCGCGGGGCGGCCCCCCTG
CCGCGGCGCGGCACCGAGCGCCCCAGCCACCGTCGTGGCACTGGGCTCCCGGCCAAAGGCGCCCCCCCTC
GCCACCATCAGCGAAGAATAA

Protein Information

Protein Name

envelope glycoprotein D

NCBI Protein GI

9629886

Protein Accession

NP_045370

Protein pI

4.96

Protein Weight

42542.47

Protein Length

417

Protein Note

envelope glycoprotein D; the Herpesviridae are non-segmented dsDNA viruses with genomes ranging from 120-230kbp; although herpes viruses vary greatly in sequence identity and homology, they all share four common elements: an envelope, a tegument which is composed of viral enzymes, a capsid of 162 capsomers, and a core composed of genomic DNA;virion envelope glycoproteins bind to cellular receptors; the nonessential glycoprotein gC interacts with cell surface proteoglycans, whereas the essential glycoprotein gD is involved in stable secondary attachment

Protein Sequence

>NP_045370.1 envelope glycoprotein D [Bovine alphaherpesvirus 1]
MQGPTLAVLGALLAVAVSLPTPAPRVTVYVDPPAYPMPRYNYTERWHTTGPIPSPFADGREQPVEVRYAT
SAAACDMLALIADPQVGRTLWEAVRRHARAYNATVIWYKIESGCARPLYYMEYTECEPRKHFGYCRYRTP
PFWDSFLAGFAYPTDDELGLIMAAPARLVEGQYRRALYIDGTVAYTDFMVSLPAGDCWFSKLGAARGYTF
GACFPARDYEQKKVLRLTYLTQYYPQEAHKAIVDYWFMRHGGVVPPYFEESKGYEPPPAADGGSPAPPGD
DEAREDEGETEDGAAGREGNGGPPGPEGDGESQTPEANGGAEGEPKPGPSPDADRPEGWPSLEAITHPPP
APATPAAPDAVPVSVGIGIAAAAIACVAAAAAGAYFVYTRRRGAGPLPRKPKKLPAFGNVNYSALPG

Vaxign Prediction

Localization(Probability)

(Prob.=0)

Adhesin Probability

0.549

Trans-membrane Helices

Detailed Vaxign Results

Vaxign Results

Epitope Information

IEDB Linear Epitope