In agreement with the data for serum IgG anti toxin immunogenicity, it appears that the construct producing the secreted form of the Stx1B EspP fusion was the most protective against challenge with a virulent Stx1 toxin producing rEPEC strain of a different serogroup. (Byrd et al., 2017)
Vaccination against both PNAG and Stx, using a construct such as the 9GlcNH2-Stx1b conjugate vaccine, would be protective (Lu et al., 2014).
References
Byrd et al., 2017: Byrd W, Ruiz-Perez F, Setty P, Zhu C, Boedeker EC. Secretion of the Shiga toxin B subunit (Stx1B) via an autotransporter protein optimizes the protective immune response to the antigen expressed in an attenuated E. coli (rEPEC E22?ler) vaccine strain. Veterinary microbiology. 2017; 211; 180-188. [PubMed: 29102116].
Lu et al., 2014: Lu X, Skurnik D, Pozzi C, Roux D, Cywes-Bentley C, Ritchie JM, Munera D, Gening ML, Tsvetkov YE, Nifantiev NE, Waldor MK, Pier GB. A Poly-N-acetylglucosamine-Shiga toxin broad-spectrum conjugate vaccine for Shiga toxin-producing Escherichia coli. mBio. 2014; 5(2); 00974-00914. [PubMed: 24667709].
