VIOLIN: Vaccine Investigation and Online Information Network

FlaA from C. jejuni 81-176

General Information

Protegen ID

398

Sequence Strain (Species/Organism)

Campylobacter jejuni subsp. jejuni 81-176

VO ID

VO_0011046

Taxonomy ID

354242

Molecule Role

Protective antigen

Molecule Role Annotation

Mice were immunized intranasally with two doses of 3 to 50 microgram of MBP-FlaA. The protective efficacies of a 50 microgram of MBP-FlaA plus LT(R192G) dose against disease symptoms and intestinal colonization were 81.1 and 84%, respectively. When mice which had been immunized with 50 microgram of MBP-FlaA plus LT(R192G) intranasally were challenged orally with 8 x 10(10), 8 x 10(9), or 8 x 10(8) cells of strain 81-176, the protective efficacies against intestinal colonization at 7 days postinfection were 71.4, 71.4, and 100%, respectively (Lee et al., 1999).

COG

COG1344N, under N: Cell motility

Related Vaccines(s)

C. jejuni MBP-FlaA protein vaccine

References

Lee et al., 1999: Lee LH, Burg E 3rd, Baqar S, Bourgeois AL, Burr DH, Ewing CP, Trust TJ, Guerry P. Evaluation of a truncated recombinant flagellin subunit vaccine against Campylobacter jejuni. Infection and immunity. 1999 Nov; 67(11); 5799-805. [PubMed: 10531231].

Gene Information

Gene Name

FlaA from C. jejuni 81-176

NCBI Gene ID

4682159

Genbank Accession

AY102622

Locus Tag

CJJ81176_1339

Gene Starting Position

1255199

Gene Ending Position

1256929

DNA Sequence

>NC_008787.1:1255199-1256929 Campylobacter jejuni subsp. jejuni 81-176, complete genome
ACTATTGTAATAATCTTAAAACATTTTGCTGACTAGAATTTGCTTGAGCCATTGCATAAGAACCACTTTG
GGCTAAGATGTTAGCTTTAGAGTAGTTTGCACTCTCACTTGCAAAGTCTACATCTCTGATTTGCGATTCT
GCTGCTTTAACATTAACTTGAGTTACAGTAATGTTATTTATAGTTGATGTAACTTGATTTTGTATAGAAC
CAATATCTGCTCTGATTTGATCTAGATTTGTTATAGCAGTTTCTGCTATATCCATAACCGCCATTGCACC
TTTAAGAGTGGTTACACCTGCAGTCTCATCAGTTGTATTAGCAGCAGTAGTTTTAAGGGCTGCAAATTGA
GAATTTCCTGAGCCAGAAGAAAATCCTGAACCTGCTGAAACAACATAAGTATTGCTCATTGAAGCCGCAC
TTGAAATAATTTGTATTCCTTGACTCAATCCAACAGATAAATTTTTACCACTACCCACAGAAAATCCTGA
ACCTCTAGAAAATCCTGAACCTTGTGCACTCATAAATGCAGAAATTGAACTTACATTTTGAGTAAAAACA
AATTTTCCACCACCTTTATAAGAATTAAATCCCATAGCATCGGCATTGGTTGCTGAAATTTGACCTTTTG
ATTCTCTTAAAGACACTGAAGATTGAGAAATCATATCTGTTGTACCCATACCTATAGCACTAAGATTGGT
TCCACTTATATTGATATCTCTACCATCATTTTTAACTAAAGATAATCGCCCATAGTTTTCTTTTTGATTT
GCCAAAATACCAGAACCAACACCTATATCTCCAGTAATTTTAATACCCCTGCCATCAGCCGATGTAAGAA
CAAGCTTGCCGTTTTCATCTTTAGAAGCTTGAACTCCTGTGGTATCTTTAACCGCATTGATAGCTGAAAT
CAAAGAGCCGTTACCATCTCCGTCTTTGTATTCAATTTTTCCTATAGTTACTCCATTAATGGCAAATTCT
TGAGAAGTAGTTCCTTCTTTTATAGCATAAACGCCAGTTGTTTTTACATCGTAAGTTGCGCGAACTCCTG
TTTTATCAGCGCTTTTATTGATCTCTTCAGCCAAAGCTCCAAGTCCTGTTCCAACTGAAGTTGAAATCAC
AACATTATCAAATTTAAAATCTTCTATACCATTGTAGTTTTTAATAGTAAGACCAACCACACCTGAAGTA
AAACTTTGAGCACCGGTTTCAAATCTTGTAACACCGATTTTAGAAGATTGAGTAGCACCGATAGTTGCTT
TCACAGTTTGGTTTGAACTTGCGCCGATTTGGAATTCTTGATTGGTAAAATTTCCACTTAAAAGTTGCTT
ACCATTAAATGAAGTAGTATTTGCGATATTATCAAGCTCTTCCATTAATTTATTAATATCTGCTTGAAGC
ATAGTTCTTGTTTTTAAACTTTGTCCATCTTGAGCTGCTTGAGTAGCTTTAGTCTTGATAGTATCAAGAA
TTTTTAATTGCTCATCCATAGCTTTATCTGCGGTTTGTAAGATACCTAAAGCATCATTACCATTTGATAT
AGCTTGACCTAAAGTATTTGCTTGAGATCTTAAGCTATCTGCTATCGCCATCCCTGAAGCATCATCTGCT
GCTGAGTTAATTCTAAGACCTGAACTAAGTCTGCTTAAAGAAGCATCTAAACTTTTAGCATTAAGATCAG
AGTTTGCTTTTGCATTCAATGCTGCAACATTTGTGTTAATACGAAATCCCA

Protein Information

Protein Name

flagellin

NCBI Protein GI

121612545

Protein Accession

YP_001000997

Protein pI

5.37

Protein Weight

56366.68

Protein Length

576

Protein Note

FlaA; structural flagella protein; in Helicobacter the flagella are composed of flagellin A and flagellin B; the amounts of each seem to be controlled by environmental conditions

Protein Sequence

>YP_001000997.1 flagellin [Campylobacter jejuni subsp. jejuni 81-176]
MGFRINTNVAALNAKANSDLNAKSLDASLSRLSSGLRINSAADDASGMAIADSLRSQANTLGQAISNGND
ALGILQTADKAMDEQLKILDTIKTKATQAAQDGQSLKTRTMLQADINKLMEELDNIANTTSFNGKQLLSG
NFTNQEFQIGASSNQTVKATIGATQSSKIGVTRFETGAQSFTSGVVGLTIKNYNGIEDFKFDNVVISTSV
GTGLGALAEEINKSADKTGVRATYDVKTTGVYAIKEGTTSQEFAINGVTIGKIEYKDGDGNGSLISAINA
VKDTTGVQASKDENGKLVLTSADGRGIKITGDIGVGSGILANQKENYGRLSLVKNDGRDINISGTNLSAI
GMGTTDMISQSSVSLRESKGQISATNADAMGFNSYKGGGKFVFTQNVSSISAFMSAQGSGFSRGSGFSVG
SGKNLSVGLSQGIQIISSAASMSNTYVVSAGSGFSSGSGNSQFAALKTTAANTTDETAGVTTLKGAMAVM
DIAETAITNLDQIRADIGSIQNQVTSTINNITVTQVNVKAAESQIRDVDFASESANYSKANILAQSGSYA
MAQANSSQQNVLRLLQ

Epitope Information

IEDB Linear Epitope