Immunoblots of iron-restricted profiles detected PotD2, heme-binding protein A (HbpA), and capsule polysaccharide export protein (CpxD) as well as surface antigens TbpA, TbpB, and OmlA. These data identify OM proteins that demonstrate immunogenicity and upregulation under conditions mimicking infection.(Chung et al., 2012)
References
Chung et al., 2012: Chung JW, Küster-Schöck E, Gibbs BF, Jacques M, Coulton JW. Immunoproteomic analyses of outer membrane antigens of Actinobacillus pleuropneumoniae grown under iron-restricted conditions. Veterinary microbiology. 2012; 159(1-2); 187-194. [PubMed: 22541161].
Gene Information
Gene Name
potD2
Protein Information
Protein Name
spermidine/putrescine-binding periplasmic protein 1 precursor
The substrate binding domain of LysR-type transcriptional regulators (LTTRs), a member of the type 2 periplasmic binding fold protein superfamily; cl25412
Protein Sequence
>ABN73472.1 spermidine/putrescine-binding periplasmic protein 1 precursor [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MKKLAGLFAAGLATVALTACNEEKPKAAEAAAQPAAAGTVHLYTWTEYVPEGLLDEFTKQTGIKVEVSSL
ESNETMYAKLKLQGKDGGYDVIAPSNYFVSKMAKEGMLAELDHAKLPVIKELNQDWLNKPYDQGNKYSLP
QLLGAPGIAFNSNDYKGDAFTSWGDLWKPEFANKVQLLDDAREVFNIALLKLGKNPNTTNPEEIKAAYEE
LRKLRPNVLSFTSDNPANSFIAGEVSVGQLWNGSVRIAKKEQAPVNMVFPKEGPVLWVDTLAIPANAKNK
ENAHKLINYLLSAPVAEKLTLEIGYPTSNVEALKTLPKEITEDPAIYPTADVLKAAQWQDDVGNAIELYE
KYYQELKAAK
