VIOLIN: Vaccine Investigation and Online Information Network

RplF

General Information

Protegen ID

437

Sequence Strain (Species/Organism)

Chlamydia muridarum str. Nigg

VO ID

VO_0010892

Taxonomy ID

243161

Molecule Role

Protective antigen

Molecule Role Annotation

Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, RplF was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).

Related Vaccines(s)

C. muridarum RplF protein vaccine

References

Yu et al., 2009: Yu H, Jiang X, Shen C, Karunakaran KP, Brunham RC. Novel Chlamydia muridarum T cell antigens induce protective immunity against lung and genital tract infection in murine models. Journal of immunology (Baltimore, Md. : 1950). 2009; 182(3); 1602-1608. [PubMed: 19155509].

Gene Information

Gene Name

RplF

NCBI Gene ID

1246168

Genbank Accession

AE002160

Locus Tag

TC_RS04070

Gene Starting Position

941594

Gene Ending Position

942145

DNA Sequence

>NC_002620.2:941594-942145 Chlamydia muridarum Nigg, complete genome
TCTATTTTTTACCAGTTTTCGCTGCTTTTCCAGCCTTACGACGTACATATTCGTTTTCGTAACGAATTCC
TTTACCTTTGTATGGCTCTGGAGGGCGTTTTGCACGAACACAAGCCGCAAATTCTCCTACCAACTGCTTG
TTGATACCTTTGATGGAGATCAATGTGTTCTTCTCAACAGAGACTTCCAATCCTGTAGGAATAGGCATTT
TTGTTGGATGGGAAACCCCTATCGATAAATCTAAGAAGGACCCCTGTACCGAAGCTCTGAATCCAACTCC
GATCATTTCTAAACGTTTTTCAAATCCAAGGTGGACACCTTTGACCATATTTGCTATCAAAGCCCAATAA
AGCCCTTGCATACGACCAGGTCTGTCTATAATGTGAGCTGCGGGAGAAACAAACACCTCATTACCTTTAA
CGGCAATCTCAACTTCTTTAGCCAACACCTGTGTCAAAGACCCTTTAGGACCTTTTACTGAGATTTCATC
ATTTTGAATAGAGACCTCTACGCCTTGAGGAAGTACAATAGGGTCTCGAGCTTTACGAGACA

Protein Information

Protein Name

50S ribosomal protein L6

NCBI Protein GI

497917443

Protein Accession

WP_010231599

Protein pI

10.64

Protein Weight

18060.75

Protein Length

183

Protein Note

ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance

Protein Sequence

>WP_010231599.1 50S ribosomal protein L6 [Chlamydia muridarum]
MSRKARDPIVLPQGVEVSIQNDEISVKGPKGSLTQVLAKEVEIAVKGNEVFVSPAAHIIDRPGRMQGLYW
ALIANMVKGVHLGFEKRLEMIGVGFRASVQGSFLDLSIGVSHPTKMPIPTGLEVSVEKNTLISIKGINKQ
LVGEFAACVRAKRPPEPYKGKGIRYENEYVRRKAGKAAKTGKK

Epitope Information

IEDB Linear Epitope