VIOLIN: Vaccine Investigation and Online Information Network

PknD

General Information

Protegen ID

439

Sequence Strain (Species/Organism)

Chlamydophila pneumoniae CWL029

VO ID

VO_0010894

Taxonomy ID

115713

Molecule Role

Protective antigen

Molecule Role Annotation

IM-ID vaccination with CPn0095 (pknD) generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of CPN0095 to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).

Related Vaccines(s)

C. pneumoniae DNA vaccine encoding PknD

References

Li et al., 2006: Li D, Borovkov A, Vaglenov A, Wang C, Kim T, Gao D, Sykes KF, Kaltenboeck B. Mouse model of respiratory Chlamydia pneumoniae infection for a genomic screen of subunit vaccine candidates. Vaccine. 2006; 24(15); 2917-2927. [PubMed: 16434129].

Gene Information

Gene Name

PknD

NCBI Gene ID

895704

Genbank Accession

AE001363

Locus Tag

CPn0095

Gene Starting Position

115994

Gene Ending Position

118792

DNA Sequence

>NC_000922.1:115994-118792 Chlamydophila pneumoniae CWL029 chromosome, complete genome
TTTGGAGCGCTATGATATTGTTAGAATTATTGGAAAGGGAGGCATGGGTGAAGTCTATCTTGCCTACGAT
CCTGTATGTTCTCGTAAAGTAGCTCTTAAAAAAATTCGTGAAGATCTTGCAGAAAATCCTCTTTTGAAAA
GGAGGTTTTTACGAGAGGCAAGAATTGCCGCTGACCTTATTCATCCTGGTGTTGTTCCTGTCTATACTAT
TTACAGCGAGAAAGATCCTGTATACTACACGATGCCCTACATAGAGGGATATACACTAAAAACCTTACTG
AAGAGTGTATGGCAAAAGGAATCCCTGTCTAAGGAATTAGCAGAGAAAACTTCTGTAGGGGCATTTCTTT
CTATCTTTCATAAGATCTGCTGCACTATAGAATATGTCCATTCTCGGGGCATTCTTCATCGCGACCTTAA
ACCCGATAACATCTTATTAGGTCTTTTTAGTGAGGCTGTAATCTTAGATTGGGGAGCAGCAGTTGCCTGT
GGAGAAGAAGAGGATCTTCTTGATATAGATGTCAGCAAAGAGGAGGTGCTCTCTTCAAGAATGACAATTC
CAGGAAGAATAGTAGGGACTCCAGATTATATGGCTCCTGAGAGGCTCCTGGGCCATCCAGCTTCTAAAAG
TACAGACATTTATGCTTTAGGAGTGGTTCTTTATCAGATGCTCACTCTCTCTTTTCCTTATAGAAGAAAA
AAAGGAAAGAAAATAGTTCTTGACGGTCAGAGAATTCCAAGTCCTCAAGAGGTAGCTCCTTATCGAGAAA
TCCCTCCGTTTCTTTCCGCTGTAGTGATGAGAATGTTGGCTGTAGATCCTCAAGAGCGCTATTCTTCGGT
AACAGAGCTTAAGGAAGATATCGAGAGTCATCTGAAAGGGAGTCCTAAATGGACTTTAACCACAGCCCTG
CCACCTAAAAAATCTTCTAGTTGGAAGCTAAACGAACCTATTTTACTTTCTAAGTATTTTCCAATGTTGG
AGGTCTCTCCAGCGTCATGGTACAGTTTAGCAATCTCTAATATTGAGAGTTTTTCTGAGATGCGCTTGGA
GTATACTCTTTCTAAAAAAGGCTTGAACGAAGGCTTTGGTATTTTACTTCCCACGTCAGAAAATGCTTTA
GGGGGAGATTTTTACCAGGGGTATGGCTTTTGGCTGCATATTAAGGAGAGAACCTTATCCGTGTCTCTGG
TGAAAAATAGCCTAGAAATCCAGAGGTGCTCTCAAGATTTGGAATCTGATAAAGAGACCTTCTTGATAGC
TTTAGAGCAGCATAATCATAGTTTATCTTTGTTTGTCGATGGTACGACTTGGCTTATCCATATGAATTAT
CTGCCAAGTCGTAGTGGGCGAGTCGCTATCATAGTTCGCGATATGGAAGATATCCTGGAAGATATAGGCA
TTTTTGAAAGTAGTGGCTCTTTGAGGGTCAGTTGTCTTGCTGTTCCTGACGCTTTTCTTGCTGAGAAGTT
ATATGATCGCGCTTTAGTGCTTTACCGAAGGATCGCAGAATCTTTCCCAGGACGTAAAGAAGGTTATGAA
GCAAGGTTCAGAGCAGGAATTACAGTTTTAGAGAAGGCCTCTACAGATAATAATGAACAGGAATTTGCTC
TAGCCATTGAAGAATTCTCAAAATTACATGACGGGGTTGCTGCTCCCTTAGAATACCTTGGTAAGGCTTT
AGTATATCAGAGACTCCAAGAGTATAATGAAGAAATTAAGAGTTTGCTATTAGCATTGAAACGTTATTCG
CAGCATCCTGAAATCTTTAGGCTTAAAGACCATGTGGTTTACCGACTCCATGAGAGCTTTTATAAACGGG
ATCGCCTTGCTCTGGTGTTCATGATTTTAGTATTGGAAATAGCTCCCCAGGCAATCACTCCAGGGCAGGA
AGAAAAAATCCTGGTTTGGTTAAAGGACAAATCTCGGGCTACCTTATTTTGCCTCCTGGATCCCACGGTC
TTAGAGCTGCGCTCTTCTAAAATGGAATTATTTTTAAGTTATTGGTCTGGGTTTATTCCCCATCTCAATA
GTCTATTTCATAGAGCTTGGGATCAAAGCGATGTGCGAGCTTTGATCGAGATTTTCTATGTTGCTTGTGA
TCTTCATAAATGGCAGTTTCTCTCTTCTTGTATCGACATATTTAAAGAGTCTCTTGAGGATCAGAAAGCC
ACAGAAGAGATTGTTGAGTTCTCTTTCGAGGATTTAGGGGCATTTCTTTTTGCTATTCAGAGCATCTTTA
ACAAGGAAGATGCAGAGAAGATCTTTGTTTCTAATGATCAATTATCGCCAATCCTTCTTGTTTATATATT
CGATCTTTTTGCAAATCGTGCTCTTCTGGAATCTCAAGGAGAGGCTATTTTTCAGGCTTTGGATCTCATC
CGAAGTAAAGTTCCTGAAAATTTTTATCATGATTACTTGCGGAATCATGAAATCCGAGCGCATCTTTGGT
GCCGCAATGAGAAGGCTCTAAGCACGATTTTTGAAAACTATACAGAGAAACAGCTAAAGGATGAGCAACA
TGAACTGTTCGTTCTCTATGGATGTTACCTTGCTCTTATACAAGGTGCTGAGGCGGCAAAGCAGCATTTT
GATGTATGTCGTGAAGATCGCATTTTCCCTGCTTCATTATTAGCTAGAAATTACAATCGTTTAGGTCTTC
CCAAAGATGCTCTTAGCTATCAAGAGCGGCGTTTGTTATTGCGACAAAAGTTTCTCTATTTCCATTGTCT
TGGTAACCACGACGAGCGTGACTTATGCCAGACTATGTATCACCTCTTAACCGAAGAATTTCAGCTTTA

Protein Information

Protein Name

serine/threonine protein kinase

NCBI Protein GI

161353778

Protein Accession

NP_224303

Protein pI

6.03

Protein Weight

102446.37

Protein Length

932

Protein Note

PknD; responsible for phosphorylation of proteins on serine and threonine residues; similar to eukaryotic Ser/Thr kinases; in Chlamydia trachomatis itseems to interact with Pkn1, another serine/threonine-protein kinase

Protein Sequence

>NP_224303.2 serine/threonine protein kinase [Chlamydia pneumoniae CWL029]
MERYDIVRIIGKGGMGEVYLAYDPVCSRKVALKKIREDLAENPLLKRRFLREARIAADLIHPGVVPVYTI
YSEKDPVYYTMPYIEGYTLKTLLKSVWQKESLSKELAEKTSVGAFLSIFHKICCTIEYVHSRGILHRDLK
PDNILLGLFSEAVILDWGAAVACGEEEDLLDIDVSKEEVLSSRMTIPGRIVGTPDYMAPERLLGHPASKS
TDIYALGVVLYQMLTLSFPYRRKKGKKIVLDGQRIPSPQEVAPYREIPPFLSAVVMRMLAVDPQERYSSV
TELKEDIESHLKGSPKWTLTTALPPKKSSSWKLNEPILLSKYFPMLEVSPASWYSLAISNIESFSEMRLE
YTLSKKGLNEGFGILLPTSENALGGDFYQGYGFWLHIKERTLSVSLVKNSLEIQRCSQDLESDKETFLIA
LEQHNHSLSLFVDGTTWLIHMNYLPSRSGRVAIIVRDMEDILEDIGIFESSGSLRVSCLAVPDAFLAEKL
YDRALVLYRRIAESFPGRKEGYEARFRAGITVLEKASTDNNEQEFALAIEEFSKLHDGVAAPLEYLGKAL
VYQRLQEYNEEIKSLLLALKRYSQHPEIFRLKDHVVYRLHESFYKRDRLALVFMILVLEIAPQAITPGQE
EKILVWLKDKSRATLFCLLDPTVLELRSSKMELFLSYWSGFIPHLNSLFHRAWDQSDVRALIEIFYVACD
LHKWQFLSSCIDIFKESLEDQKATEEIVEFSFEDLGAFLFAIQSIFNKEDAEKIFVSNDQLSPILLVYIF
DLFANRALLESQGEAIFQALDLIRSKVPENFYHDYLRNHEIRAHLWCRNEKALSTIFENYTEKQLKDEQH
ELFVLYGCYLALIQGAEAAKQHFDVCREDRIFPASLLARNYNRLGLPKDALSYQERRLLLRQKFLYFHCL
GNHDERDLCQTMYHLLTEEFQL

Epitope Information

IEDB Linear Epitope