VIOLIN: Vaccine Investigation and Online Information Network

botA

General Information

Protegen ID

447

VO ID

VO_0010902

Taxonomy ID

32630

Other Database IDs

CDD:285228
CDD:285226

Molecule Role

Protective antigen

Molecule Role Annotation

A completely synthetic gene encoding fragment C, a approximately 50-kDa fragment, of botulinum neurotoxin serotype A was constructed from oligonucleotides. Mice given three subcutaneous vaccinations were protected against an intraperitoneal administration of 10^6 50% lethal doses (ID50) of serotype A toxin (Clayton et al., 1995).

References

Clayton et al., 1995: Clayton MA, Clayton JM, Brown DR, Middlebrook JL. Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in Escherichia coli. Infection and immunity. 1995; 63(7); 2738-2742. [PubMed: 7790092].

Gene Information

Gene Name

botA

Protein Information

Protein Name

botulinum neurotoxin serotype A Hc fragment

NCBI Protein GI

733429

Protein pI

9.45

Protein Weight

49259.71

Protein Length

524

Protein Note

based on Clostridium botulinum Type A neurotoxin sequence

Protein Sequence

>AAA80610.1 botulinum neurotoxin serotype A Hc fragment [synthetic construct]
MARLLSTFTEYIKNIINTSILNLRYESNHLIDLSRYASKINIGSKVNFDPIDKNQIQLFNLESSKIEVIL
KNAIVYNSMYENFSTSFWIRIPKYFNSISLNNEYTIINCMENNSGWKVSLNYGEIIWTLQDTQEIKQRVV
FKYSQMINISDYINRWIFVTITNNRLNNSKIYINGRLIDQKPISNLGNIHASNNIMFKLDGCRDTHRYIW
IKYFNLFDKELNEKEIKDLYDNQSNSGILKDFWGDYLQYDKPYYMLNLYDPNKYVDVNNVGIRGYMYLKG
PRGSVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVVKNKEYRLATNASQAGVEKILSA
LEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGNDIGFIGFHQFNNIAKLVASNWYNRQIERSSRTL
GCSWEFIPVDDGWGERPL

Epitope Information

IEDB Linear Epitope