BoNT/B
General Information
Protegen ID
449
Sequence Strain (Species/Organism)
Clostridium botulinum B1 str. Okra
VO ID
VO_0010904
Taxonomy ID
498213
Other Database IDs
CDD:279999
Molecule Role
Protective antigen
Molecule Role Annotation
The DNA replicon vaccine (pSCARSBHc) encoding the Hc domain of BoNT/B (BHc) induced better responses and protection against BoNT/B mouse challenge than conventional DNA vaccine. Recombinant SFV particle VRP-BHc protected mice from high-dose BoNT/B challenge (Yu et al. , 2009 ).Webb et al. , 2017 )
Related Vaccines(s)
C. botulinum DNA vaccine pSCARSA/BHc
References
Webb et al. , 2017: Webb RP, Smith TJ, Smith LA, Wright PM, Guernieri RL, Brown JL, Skerry JC. Recombinant Botulinum Neurotoxin Hc Subunit (BoNT Hc) and Catalytically Inactive Clostridium botulinum Holoproteins (ciBoNT HPs) as Vaccine Candidates for the Prevention of Botulism. Toxins . 2017; 9(9); . [PubMed: 28869522 ].Yu et al. , 2009: Yu Y, Yu J, Li N, Wang S, Yu W, Sun Z. Individual and bivalent vaccines against botulinum neurotoxin serotypes A and B using DNA-based Semliki Forest virus vectors. Vaccine . 2009; 27(44); 6148-6153. [PubMed: 19712769 ].
Gene Information
Gene Name
BoNT/B
NCBI Gene ID
6030102
Genbank Accession
AB232927
Locus Tag
CLD_A0068
Plasmid No
pCLD
Gene Starting Position
42316
Gene Ending Position
46191
DNA Sequence
>NC_010379.1:42316-46191 Clostridium botulinum B1 str. Okra plasmid pCLD, complete sequence
ATTATTCAGTCCACCCTTCATCTTTAGGAATAAACTGCCAATTACATCCCAATTTTAAATTATATGGTTT
CCTTTTTACCTCTTTTAAGTACCATTTACTTATACAAAAATAATCTTTATACTCTTCAAATACAATTCCA
GATTCGTAGAAACGATGAATACCAATCAATCCTATCTCATCAGTACTTTCTTCATCTTTTTTAAAAAGCA
ACTGACAACTATATGTTGGCTGTTCATCATATTCTTTTATTTGTATAGTATTGTAAAACTCATCAGAATC
ACTTATAGGAGCTAAAAACAATTTTTCTTCCTCTTTCTTAAAATATTTATAGGTATATACTCTCCACTCT
TGATTTAAATTAAAAAAATCTAGATATATATAATCTTCTTTTCTAACTATATCATCATTTATAGATTGAG
AATTTGACTTTCTTCTTATAATAAATTTTTCTCCAATATATAAATCTCTATAATTTATATATTTAGAATT
TTGATTATATTTGCTACGTGTTAAAATTTCACCTACAGGTGAATCTTTCTTTAGTTTAATATATGAATTT
TTATTCCCCGCATTAAACATATAATATTCTTTATTGTACATTAAAGGATTTCCCCAAAAATCTTTTAAAT
ATTCGCTATATGATTGAATTTTATATCTTTCTTCAATATTTGATTGACTTAATTCCGTATTAAAAATACT
GAAATATTTCATCCAAATAAATTGTGTTCTATCTATATCACCATCTAATTTAAATATTATTTCACCATTA
GCAATAACTTCTCTTATATCTTTAATATCTGTATTTGATTCTAGCTTACCATTAATATAAATTTTAGCGT
TATTCAAATTATTAGTAATAGTTACAAAAAACCATCTATTTATATACTCTGATATATCTTCTCTTATGTT
ATATTCAAAAAATACCGATTTGGTTTTTCCATTTATATCAATTAAAGTCCATATTATCCTATTACCCCTA
ATAGATATTTTCCAGCCCGAATTATTTTTCATACAATTAATTATTGTATATTCATTATGAATATAATTTT
GTATACCATCATTCTTATATTTAGGTATTCTTATCCAAAAGCTAACGCTAAAATCAAGGAACACACTATT
AAATATGATATTCTGATTTTGAGTCACTCTAATCTTACTATTTGCTGAACTAGTTAATTTAAATTGATTT
TTATCATTAAGCTCGACTCCATCATATACCTCTACCTTTGCCCCATATCCTGATAAATCTATTAAATTAT
TATCCTTATATCTTAAATTTAAGATAATATTATTTAAAATTTCGCTATTATATTTATTAAACATTTCTAT
TAGTATTGTATCATTGGTATATATTGAAAGATCAAACGGCATAATGGTTTTCAAGTATTTATTTACTTTT
GATTTTTCATATTCTGCACTTCCAATCAAATATAATTTATTTTCATCTATATAATTTAACAAATTTTTTT
TGAGAGTATTATCAAAGTCTAGTAATTTTTCTACAGCTAATGGAATCATTTTTTTCATTAAATATGATAC
AGAACATCCATTTATAAAATTATTTATATTATCTATAGCTTGGTTAATACCCTCATTAAGTTTAGAATTT
ATATCATTAAAATCGATGTTAATATTTGACTTTTCTTTTTCAGAATATATATTATATCTGTATTTTATTA
TTTCTTCCAATGCTTGTGCTTGATAATTTAAAGCCTTATACATTCCCTCTTTTATTGTATAAAATTGAGT
ATTAACTGTTGAGAGCCATTGCGCTACTATTAATCCGTACATATCACTCCATTTTTCATTTCTTTTAGTT
AAAGCATTATCTATTGTTTTAATAATTTTATTTTTATTGTCAATATATGATTCTAATAAAAAGGCTCCAA
CTACAGGTATTAAAAGTTCTGGTATAAATTCTAGTAGAATACTGGCTCCTGCAATCTCAAAAGCATTTTC
AAAATTTCCTTTAGCTGTTTCATTTCCTACATTTAAAGCTAATCCTATATAAGGAACAATTAGAGATATA
TCTGCAATTTTATCCATAGTATTGCTTTTATTAGCTTCGATTACAAAATCATTTACTATCTGTTTCACCC
AACCTGCAAATAATCCTGCTTCTACCACTTTATTAGCAGTTTTAATATAATCCATAGAAAAAAATGAATA
AACTTTGTTAGAAAATAATAATGCATCATCAAATGAAGATGTTAAACTTATATCTCTTATATCTAGAGGA
AATGTCTGAGAGTATAAATATTGAAAGATGGTATTTTCATCTGTAAAAATTTTTTTTATAGCGGGTTGTT
TTTCATATACTGGAACATCTACATTAAAATCAGTAAGTGATTCTGTATTTTCACTTGGTAATTCTATTTT
ACTTATTAAATCAGTATCTAAAATTAATTCATTTATAGGGAAGTCATTTTCTATATAATTACTCTGTGTA
TTATATTCTATTCTTTCGTTTTTAGATAAATCATCTGAAAAACTATTTTTATCAGCTATAAAGAACAAAT
CTTCATTATCAACATCAATACATATTCCTGGAGCTTTAACACTTTTACACATTTGTATCTTATATACAGC
CAAATGCTCCTTGCTAATTTCTTCATAAGCTTGTTTATTTATAGCTTTATTCTGACCTCTATATTCTTTT
TCCATATCTTTATCAGATATATTAAACCCTTCCTCTATAGTATAGATTTCATTATCTAATAAATTTTTTA
TTTTTACTGGTGGTAAGGAATCACTAAAATAAGAAGCTCTAGTTTTTATTTTATAATTTTCTGCTATATT
AGTTTCTGTAAAACCAAACATTAAGCTTTTATATAATTTATCAAAACTTTCTACATCTATACTATATTTT
CCCTCAGAATCTTCAACGAATTTATATTTATCTTTAAATTTATTTTTATATATATTAATATTAATGTTAG
GATCTGATATGCAAACTAAAACCTTGTTAAGTCTATCAACTATCCCTCTAAAATTTTGCAAAACTTTATC
ATAGATACTTTTATCCGTAGAAGGAGTTATGATGCTGGGATCTTGTCCTCCAAATGTATATAGTTCTTCT
GCCTGTATAGCATCTGTAGATTGCATAAAAAATTTTTTTTCATTTGGTACAATTGGTAAATCATCTACTT
TAATGCCATATAATCCATGTAAAACATGTATAAGTTCATGCATTAATATCAAGGCTGGATCTGAAAAATA
TCCACGTCTATTAAATATACTTGCGCCTTTGTTTTCTTGAACATTATTAAATACGCTTACATATTCTGGG
CAAAACTTCATTTGCATTATACCCCCGAAGCCTTCCCTTGATGCAAAATGATTTTGTATACCTATATCTA
TAGTCTCATTTTCATTTAAAACTGGCCCAGGTCCAAATATTATTAAATTTGCGAAAATACCTTTTTTTCG
CTCCACTTCTCCTGGATTACTGATTAATTTATTAACAGTTACACTAGCAATGTTTGTGTTAAACTCTTCG
AGTGGAACACGTCTATCTCCAAGATAAGGTATACCATTTATAATCATCTCTAATAACTTTTCACCCAATG
GTTTTGATTTGATTCTATTAAATAACTTGATCATTGTTTGTAAAAATATATTCTTTTTATCATTAGTATT
TAAGTAATCTGGATCATAATATTCACAAACATCTCTATTAAAAATACCGGAACTTTTATTAAAATCCTCA
GGTTTATATCCAAAAGTATATCTTTCCGGTATTATCCAAATACGATCTGTGATTTTAAAAGCTTTATAAT
ATCTCCCCGTACCTCTCGCAAATGGAGGCTCCATCATAATAATATTATTATTATCAATAGGATCATTATA
ATTAAAATTATTTATTGTAACTGGCA
Protein Information
Protein Name
botulinum neurotoxin type B
NCBI Protein GI
169834607
Protein Accession
YP_001693307
3D structure: PDB ID
1S0F
Protein pI
4.97
Protein Weight
145339.54
Protein Length
1291
Protein Note
Clostridial neurotoxin zinc protease; pfam01742
Protein Sequence
>YP_001693307.1 botulinum neurotoxin type B (plasmid) [Clostridium botulinum B1 str. Okra]
MPVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWIIPERYTFGYKPEDFNKSSGIFNRDV
CEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPLGEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNK
LISNPGEVERKKGIFANLIIFGPGPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENK
GASIFNRRGYFSDPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSII
TPSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSIDVESFDKLYK
SLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFNISDKDMEKEYRGQNKAINKQA
YEEISKEHLAVYKIQMCKSVKAPGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINE
LILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFD
DALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNV
GNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLI
VAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDN
INNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDL
SIYTNDTILIEMFNKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELNDKNQFKLTSSANSKIR
VTQNQNIIFNSVFLDFSVSFWIRIPKYKNDGIQNYIHNEYTIINCMKNNSGWKISIRGNRIIWTLIDING
KTKSVFFEYNIREDISEYINRWFFVTITNNLNNAKIYINGKLESNTDIKDIREVIANGEIIFKLDGDIDR
TQFIWMKYFSIFNTELSQSNIEERYKIQSYSEYLKDFWGNPLMYNKEYYMFNAGNKNSYIKLKKDSPVGE
ILTRSKYNQNSKYINYRDLYIGEKFIIRRKSNSQSINDDIVRKEDYIYLDFFNLNQEWRVYTYKYFKKEE
EKLFLAPISDSDEFYNTIQIKEYDEQPTYSCQLLFKKDEESTDEIGLIGIHRFYESGIVFEEYKDYFCIS
KWYLKEVKRKPYNLKLGCNWQFIPKDEGWTE
Epitope Information
IEDB Linear Epitope
-- Assay Type --
T Cell Epitope
B Cell Epitope
IEDB ID
Epitope
MHC restriction
Starting position
Ending position
IEDB ID
Epitope
Starting position
Ending position
3570 APGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINELILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFDDALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNVGNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLIVAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDNINNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDLSIYTNDTILIEMFNKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELN
442
895
7097 CSVSYLMKKMIPLAVEKLL
778
797
73893 YFSIFNTELSQSNIEERYK
1058
1077
45598 NQNSKYINYRDLYIGEKFI
1128
1147
2806 ALNYQAQALEEIIKYRYNI
722
741
45124 NNIILNLRYKDNNLIDLSG
862
881
37638 LLNYIDENKLYLIGSAEYE
806
825
22949 GVELNDKNQFKLTSSANSK
890
909
28322 IRIPKYKNDGIQNYIHNEY
932
951
26132 IFNSVFLDFSVSFWIRIPK
918
937
43732 NERIEYNTQSNYIENDFPI
470
489
26394 IHNEYTIINCMKNNSGWKI
946
965
30246 KDSPVGEILTRSKYNQNSK
1114
1133
75691 YRYNIYSEKEKSNINIDFN
736
755
14983 EYDEQPTYSCQLLFKKDEE
1212
1231
8770 DIREVIANGEIIFKLDGDI
1030
1049
11870 EERYKIQSYSEYLKDFWGN
1072
1091
8265 DFFNLNQEWRVYTYKYFKK
1170
1189
19318 GEKFIIRRKSNSQSINDDI
1142
1161
27100 ILIEMFNKYNSEILNNIIL
848
867
43438 NDFPINELILDTDLISKIE
484
503
68246 VEKLLDFDNTLKKNLLNYI
792
811
26404 IHRFYESGIVFEEYKDYFC
1240
1259
57250 SDMYGLIVAQWLSTVNTQF
694
713
70203 VNTQFYTIKEGMYKALNYQ
708
727
56768 SAEYEKSKVNKYLKTIMPF
820
839
30276 KDYFCISKWYLKEVKRKPY
1254
1273
27724 INQAIDNINNFINGCSVSY
764
783
64383 TIMPFDLSIYTNDTILIEM
834
853
64843 TLIDINGKTKSVFFEYNIR
974
993
41519 MFNAGNKNSYIKLKKDSPV
1100
1119
43826 NFENAFEIAGASILLEFIP
638
657
33173 KRKPYNLKLGCNWQFIPKDEGWTE
1268
1292
28460 ISDSDEFYNTIQIKEYDEQ
1198
1217
30247 KDSPVGEILTRSKYNQNSKY
1114
1134
24673 HRFYESGIVFEEYKDYFCISKWY
1241
1264
74442 YKDYFCISKWYLKEVKRKPYNLKLG
1253
1278
44635 NKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELNDKNQFKLTSSANSKIRVTQNQNIIFNSVFLDFSVSFWIRIPKYKNDGIQNYIHNEYTIINCMKNNSGWKISIRGNRIIWTLIDINGKTKSVFFEYNIREDISEYINRWFFVTITNNLNNAKIYINGKLESNTDIKDIREVIANGEIIFKLDGDIDRTQFIWMKYFSIFNTELSQSNIEERYKIQSYSEYLKDFWGNPLMYNKEYYMFNAGNKNSYIKLKKDSPVGEILTRSKYNQNSKYINYRDLYIGEKFIIRRKSNSQSINDDIVRKEDYIYLDFFNLNQEWRVYTYKYFKKEEEKLFLAPISDSDEFYNTIQIKEYDEQPTYSCQLLFKKDEESTDEIGLIGIHRFYESGIVFEEYKDYFCISKWYLKEVKRKPYNLKLGCNWQFIPKDEGWTE
854
1292
3568 APGICIDVDNEDLFFIADK
442
461
8335 DFWGNPLMYNKEYYMFNAG
1086
1105
58312 SGWKISIRGNRIIWTLIDI
960
979
142485 YNIYSEKEK
738
747
161095 EEFNTNIASVTVNKLISNP
127
146
161081 ALILMHELIHVLHGLYGIK
225
244
161101 GASIFNRRGYFSDPALILM
211
230
161124 LYGIKVDDLPIVPNEKKFF
239
258
161126 NFRGIVDRLNKVLVCISDP
295
314
161089 CISDPNININIYKNKFKDK
309
328
161119 KYSIDVESFDKLYKSLMFG
337
356
161088 CEYYDPDYLNTNDKKNIFL
71
90
161106 IKSKPLGEKLLEMIINGIP
99
118
161108 INGIPYLGDRRVPLEEFNT
113
132
161120 LISNPGEVERKKGIFANLI
141
160
161129 PEYVSVFNNVQENKGASIF
197
216
161162 YKIKTRASYFSDSLPPVKI
365
384
161125 MPVTINNFNYNDPIDNNNI
1
20
161109 IWIIPERYTFGYKPEDFNK
43
62
161100 FANLIIFGPGPVLNENETI
155
174
161099 ENETIDIGIQNHFASREGF
169
188
161153 SREGFGGIMQMKFCPEYVS
183
202
161098 EKKFFMQSTDAIQAEELYT
253
272
161151 SLMFGFTETNIAENYKIKT
351
370
49982 PVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWIIPERYTFGYKPEDFNKSSGIFNRDVCEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPLGEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNKLISNPGEVERKKGIFANLIIFGPGPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENKGASIFNRRGYFSDPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSIITPSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSIDVESFDKLYKSLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFNISDKDMEKEYRGQNKAINKQAYEEISKEHLAVYKIQMCKSVK
2
442
45125 NNIILNLRYKDNNLIDLSGYGAKVEVYDGVELNDKNQFKLTSSANSKIRVTQNQNIIFNSVFLDFSVSFWIRIPKYKNDGIQNYIHNEYTIINCMKNNSGWKISIRGNRIIWTLIDINGKTKSVFFEYNIREDISEYINRWFFVTITNNLNNAKIYINGKLESNTDIKDIREVIANGEIIFKLDGDIDRTQFIWMKYFSIFNTELSQSNIEERYKIQSYSEYLKDFWGNPLMYNKEYYMFNAGNKNSYIKLKKDSPVGEILTRSKYNQNSKYINYRDLYIGEKFIIRRKSNSQSINDDIVRKEDYIYLDFFNLNQEWRVYTYKYFKKEEEKLFLAPISDSDEFYNTIQIKEYDEQPTYSCQLLFKKDEESTDEIGLIGIHRFYESGIVFEEYKDYFCISKWYLKEVKRKPYNLKLGCNWQFIPKDEGWTE
862
1292
175578 DVCEYYDPDYLNT
69
82
3571 APGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINELILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFDDALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNVGNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLIVAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDNINNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDLSIYTNDTILIEMFNKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELNDKNQFKLTSSANSKIRVTQNQNIIFNSVFLDFSVSFWIRIPKYKNDG
442
942
47677 PGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINELILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFDDALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNVGNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLIVAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDNINNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDLSIYTNDTILIEMFNKYNSEIL
443
862
MPVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWIIPERYTFGYKPEDFNKSSGIFNRDVCEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPLGEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNKLISNPGEVERKKGIFANLIIFGPGPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENKGASIFNRRGYFSDPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSIITPSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSIDVESFDKLYKSLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFNISDKDMEKEYRGQNKAINKQAYEEISKEHLAVYKIQMCKSVKAPGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINELILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFDDALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNVGNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLIVAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDNINNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDLSIYTNDTILIEMFNKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELNDKNQFKLTSSANSKIRVTQNQNIIFNSVFLDFSVSFWIRIPKYKNDGIQNYIHNEYTIINCMKNNSGWKISIRGNRIIWTLIDINGKTKSVFFEYNIREDISEYINRWFFVTITNNLNNAKIYINGKLESNTDIKDIREVIANGEIIFKLDGDIDRTQFIWMKYFSIFNTELSQSNIEERYKIQSYSEYLKDFWGNPLMYNKEYYMFNAGNKNSYIKLKKDSPVGEILTRSKYNQNSKYINYRDLYIGEKFIIRRKSNSQSINDDIVRKEDYIYLDFFNLNQEWRVYTYKYFKKEEEKLFLAPISDSDEFYNTIQIKEYDEQPTYSCQLLFKKDEESTDEIGLIGIHRFYESGIVFEEYKDYFCISKWYLKEVKRKPYNLKLGCNWQFIPKDEGWTE
