VIOLIN: Vaccine Investigation and Online Information Network

Pla

General Information

Protegen ID

Sequence Strain (Species/Organism)

Yersinia pestis CO92

VO ID

VO_0010877

Taxonomy ID

214092

Molecule Role

Protective antigen

Molecule Role Annotation

Study evaluated the humoral immune response of mice that survived lethal Y. pestis aerosol challenge after antibiotic treatment. Results indicated that one of the predominant antibody response of mice surviving pneumonic plague after treatment with antibiotics was to pla (Benner et al., 1999).

COG

COG4571M, under M: Cell wall/membrane/envelope biogenesis

References

Benner et al., 1999: Benner GE, Andrews GP, Byrne WR, Strachan SD, Sample AK, Heath DG, Friedlander AM. Immune response to Yersinia outer proteins and other Yersinia pestis antigens after experimental plague infection in mice. Infection and immunity. 1999; 67(4); 1922-1928. [PubMed: 10085037].

Gene Information

Gene Name

Pla

NCBI Gene ID

1172643

Genbank Accession

AL109969

Locus Tag

YPPCP1.07

Plasmid No

pPCP1

Gene Starting Position

6664

Gene Ending Position

7602

DNA Sequence

>NC_003132.1:6664-7602 Yersinia pestis CO92 plasmid pPCP1, complete sequence
AATGAAGAAAAGTTCTATTGTGGCAACCATTATAACTATTCTGTCCGGGAGTGCTAATGCAGCATCATCT
CAGTTAATACCAAATATATCCCCTGACAGCTTTACAGTTGCAGCCTCCACCGGGATGCTGAGTGGAAAGT
CTCATGAAATGCTTTATGACGCAGAAACAGGAAGAAAGATCAGCCAGTTAGACTGGAAGATCAAAAATGT
CGCTATCCTGAAAGGTGATATATCCTGGGATCCATACTCATTTCTGACCCTGAATGCCAGGGGGTGGACG
TCTCTGGCTTCCGGGTCAGGTAATATGGATGACTACGACTGGATGAATGAAAATCAATCTGAGTGGACAG
ATCACTCATCTCATCCTGCTACAAATGTTAATCATGCCAATGAATATGACCTCAATGTGAAAGGCTGGTT
ACTCCAGGATGAGAATTATAAAGCAGGTATAACAGCAGGATATCAGGAAACACGTTTCAGTTGGACAGCT
ACAGGTGGTTCATATAGTTATAATAATGGAGCTTATACCGGAAACTTCCCGAAAGGAGTGCGGGTAATAG
GTTATAACCAGCGCTTTTCTATGCCATATATTGGACTTGCAGGCCAGTATCGCATTAATGATTTTGAGTT
AAATGCATTATTTAAATTCAGCGACTGGGTTCGGGCACATGATAATGATGAGCACTATATGAGAGATCTT
ACTTTCCGTGAGAAGACATCCGGCTCACGTTATTATGGTACCGTAATTAACGCTGGATATTATGTCACAC
CTAATGCCAAAGTCTTTGCGGAATTTACATACAGTAAATATGATGAGGGCAAAGGAGGTACTCAGACCAT
TGATAAGAATAGTGGAGATTCTGTCTCTATTGGCGGAGATGCTGCCGGTATTTCCAATAAAAATTATACT
GTGACGGCGGGTCTGCAATATCGCTTCTG

Protein Information

Protein Name

outer membrane protease

NCBI Protein GI

16082686

Protein Accession

NP_395233

3D structure: PDB ID

2X56

Protein pI

6.25

Protein Weight

33320.09

Protein Length

312

Protein Note

outer membrane protease; involved in virulence in many organisms; OmpT; IcsP; SopA; Pla; PgtE; omptin; in Escherichia coli OmpT can degrade antimicrobial peptides; in Yersinia Pla activates plasminogen during infection; in Shigella flexneria SopA cleaves the autotransporter IcsA

Protein Sequence

>NP_395233.1 outer membrane protease (plasmid) [Yersinia pestis CO92]
MKKSSIVATIITILSGSANAASSQLIPNISPDSFTVAASTGMLSGKSHEMLYDAETGRKISQLDWKIKNV
AILKGDISWDPYSFLTLNARGWTSLASGSGNMDDYDWMNENQSEWTDHSSHPATNVNHANEYDLNVKGWL
LQDENYKAGITAGYQETRFSWTATGGSYSYNNGAYTGNFPKGVRVIGYNQRFSMPYIGLAGQYRINDFEL
NALFKFSDWVRAHDNDEHYMRDLTFREKTSGSRYYGTVINAGYYVTPNAKVFAEFTYSKYDEGKGGTQTI
DKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF

Epitope Information

IEDB Linear Epitope