VIOLIN: Vaccine Investigation and Online Information Network

NP from Influenza A virus (A/Puerto Rico/8/34(H1N1))

General Information

Protegen ID

528

Sequence Strain (Species/Organism)

Influenza A virus (A/Puerto Rico/8/1934(H1N1))

VO ID

VO_0011122

Taxonomy ID

211044

Molecule Role

Protective antigen

Molecule Role Annotation

A simian adenovirus 24 (AdC7) vector expressing the influenza A/Puerto Rico/8/34 NP was tested for its efficacy in protecting BALB/c mice against two H5N1 strains. When tested in a mouse challenge model, there was a significantly improved survival rate following challenge with the Vietnam/1203/04 strain of H5N1 that has caused human outbreaks (Roy et al., 2007).

Related Vaccines(s)

AdH5-FluA NP , Influenza virus DNA vaccine NP DNA , Influenza virus DNA vaccine pBK-SFV-NP , Influenza virus DNA vaccine pDNA encoding M2 and NP , Influenza virus DNA vaccine pRSV-NP encoding the coding sequence from influenza virus A/PR/8/34.

References

Roy et al., 2007: Roy S, Kobinger GP, Lin J, Figueredo J, Calcedo R, Kobasa D, Wilson JM. Partial protection against H5N1 influenza in mice with a single dose of a chimpanzee adenovirus vector expressing nucleoprotein. Vaccine. 2007; 25(39-40); 6845-6851. [PubMed: 17728024].

Gene Information

Gene Name

NP from Influenza A virus (A/Puerto Rico/8/34(H1N1))

NCBI Gene ID

956531

Genbank Accession

J02147

Locus Tag

FLUAVs5gp1

Gene Starting Position

Gene Ending Position

1541

DNA Sequence

>NC_002019.1:45-1541 Influenza A virus (A/Puerto Rico/8/1934(H1N1)) segment 5, complete sequence
CATGGCGTCCCAAGGCACCAAACGGTCTTACGAACAGATGGAGACTGATGGAGAACGCCAGAATGCCACT
GAAATCAGAGCATCCGTCGGAAAAATGATTGGTGGAATTGGACGATTCTACATCCAAATGTGCACAGAAC
TTAAACTCAGTGATTATGAGGGACGGTTGATCCAAAACAGCTTAACAATAGAGAGAATGGTGCTCTCTGC
TTTTGACGAAAGGAGAAATAAATACCTGGAAGAACATCCCAGTGCGGGGAAGGATCCTAAGAAAACTGGA
GGACCTATATACAGAAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGACAAAGAAGAAATAA
GGCGAATCTGGCGCCAAGCTAATAATGGTGACGATGCAACGGCTGGTCTGACTCACATGATGATCTGGCA
TTCCAATTTGAATGATGCAACTTATCAGAGGACAAGGGCTCTTGTTCGCACCGGAATGGATCCCAGGATG
TGCTCTCTGATGCAAGGTTCAACTCTCCCTAGGAGGTCTGGAGCCGCAGGTGCTGCAGTCAAAGGAGTTG
GAACAATGGTGATGGAATTGGTCAGGATGATCAAACGTGGGATCAATGATCGGAACTTCTGGAGGGGTGA
GAATGGACGAAAAACAAGAATTGCTTATGAAAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACTGCT
GCACAAAAAGCAATGATGGATCAAGTGAGAGAGAGCCGGGACCCAGGGAATGCTGAGTTCGAAGATCTCA
CTTTTCTAGCACGGTCTGCACTCATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGT
GTATGGACCTGCCGTAGCCAGTGGGTACGACTTTGAAAGAGAGGGATACTCTCTAGTCGGAATAGACCCT
TTCAGACTGCTTCAAAACAGCCAAGTGTACAGCCTAATCAGACCAAATGAGAATCCAGCACACAAGAGTC
AACTGGTGTGGATGGCATGCCATTCTGCCGCATTTGAAGATCTAAGAGTATTGAGCTTCATCAAAGGGAC
GAAGGTGGTCCCAAGAGGGAAGCTTTCCACTAGAGGAGTTCAAATTGCTTCCAATGAAAATATGGAGACT
ATGGAATCAAGTACACTTGAACTGAGAAGCAGGTACTGGGCCATAAGGACCAGAAGTGGAGGAAACACCA
ATCAACAGAGGGCATCTGCGGGCCAAATCAGCATACAACCTACGTTCTCAGTACAGAGAAATCTCCCTTT
TGACAGAACAACCGTTATGGCAGCATTCACTGGGAATACAGAGGGGAGAACATCTGACATGAGGACCGAA
ATCATAAGGATGATGGAAAGTGCAAGACCAGAAGATGTGTCTTTCCAGGGGCGGGGAGTCTTCGAGCTCT
CGGACGAAAAGGCAGCGAGCCCGATCGTGCCTTCCTTTGACATGAGTAATGAAGGATCTTATTTCTTCGG
AGACAATGCAGAGGAGTACGACAATTA

Protein Information

Protein Name

nucleoprotein

NCBI Protein GI

8486130

Protein Accession

NP_040982

Protein pI

9.56

Protein Weight

53786.92

Protein Length

498

Protein Sequence

>NP_040982.1 nucleoprotein [Influenza A virus (A/Puerto Rico/8/1934(H1N1))]
MASQGTKRSYEQMETDGERQNATEIRASVGKMIGGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSA
FDERRNKYLEEHPSAGKDPKKTGGPIYRRVNGKWMRELILYDKEEIRRIWRQANNGDDATAGLTHMMIWH
SNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELVRMIKRGINDRNFWRGE
NGRKTRIAYERMCNILKGKFQTAAQKAMMDQVRESRDPGNAEFEDLTFLARSALILRGSVAHKSCLPACV
YGPAVASGYDFEREGYSLVGIDPFRLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRVLSFIKGT
KVVPRGKLSTRGVQIASNENMETMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISIQPTFSVQRNLPF
DRTTVMAAFTGNTEGRTSDMRTEIIRMMESARPEDVSFQGRGVFELSDEKAASPIVPSFDMSNEGSYFFG
DNAEEYDN

Vaxign Prediction

Localization(Probability)

(Prob.=0)

Adhesin Probability

0.087

Trans-membrane Helices

Detailed Vaxign Results

Vaxign Results

Epitope Information

IEDB Linear Epitope