VIOLIN: Vaccine Investigation and Online Information Network

LASVsSgp1 nucleoprotein

General Information

Protegen ID

560

Sequence Strain (Species/Organism)

Lassa mammarenavirus

VO ID

VO_0011151

Taxonomy ID

11620

Molecule Role

Protective antigen

Molecule Role Annotation

Vaccine efficacy trials in guinea pigs indicated that the nucleoprotein is capable of eliciting a protective immune response against a lethal dose of Lassa virus. Ninety-four percent of the animals vaccinated with V-LSN survived a Lassa virus challenge in which only 14% of unvaccinated animals and 39% of animals vaccinated with the New York Board of Health (NYBH) strain of vaccinia virus survived (Morrison et al., 1989).

Related Vaccines(s)

Lassa fever Virus Ad5 (E1-, E2b-) Vectored Vaccine , Lassa fever virus recombinant vector vaccine V-LSG/N encoding the glycoprotein and the nucleoprotein , Lassa Virus Nucleoprotein Subunit Vaccine , ML29 L-AttV, rLCMV(IGR/S-S) (Mopiea/Lassa reassortant)

References

Morrison et al., 1989: Morrison HG, Bauer SP, Lange JV, Esposito JJ, McCormick JB, Auperin DD. Protection of guinea pigs from Lassa fever by vaccinia virus recombinants expressing the nucleoprotein or the envelope glycoproteins of Lassa virus. Virology. 1989; 171(1); 179-188. [PubMed: 2741340].

Gene Information

Gene Name

LASVsSgp1 nucleoprotein

NCBI Gene ID

956584

Genbank Accession

AY628203

Locus Tag

LASVsSgp1

Segment No

segment S

Gene Starting Position

100

Gene Ending Position

1809

DNA Sequence

>NC_004296.1:100-1809 Lassa virus segment S, complete sequence
GATGAGTGCCTCAAAGGAAATAAAATCCTTTTTGTGGACACAATCTTTGAGGAGGGAATTATCTGGTTAC
TGCTCCAACATCAAACTACAGGTGGTGAAAGATGCCCAGGCTCTTTTACATGGACTTGACTTCTCCGAAG
TCAGTAATGTTCAACGGTTGATGCGCAAGGAGAGAAGGGATGACAATGATTTGAAACGGTTGAGGGACCT
AAATCAAGCGGTCAACAATCTTGTTGAATTAAAATCAACTCAACAAAAGAGTATACTGAGAGTTGGGACT
CTAACCTCAGATGACTTATTAATCTTAGCCGCTGATCTAGAGAAGTTAAAGTCAAAGGTGATCAGAACAG
AAAGGCCATTAAGTGCAGGTGTCTATATGGGCAACCTAAGCTCACAGCAACTTGACCAAAGAAGAGCTCT
CCTGAATATGATAGGAATGAGTGGTGGTAATCAAGGGGCTCGGGCTGGGAGAGATGGAGTGGTGAGAGTT
TGGGATGTGAAAAATGCAGAGTTGCTCAATAATCAGTTCGGGACCATGCCAAGTCTGACACTGGCATGTC
TGACAAAACAGGGGCAGGTTGACTTGAATGATGCAGTACAAGCATTGACAGATTTGGGTTTGATCTACAC
AGCAAAGTATCCCAACACTTCAGACTTAGACAGGCTGACTCAAAGTCATCCCATCCTAAATATGATTGAC
ACCAAGAAAAGCTCTTTGAATATCTCAGGTTATAATTTTAGCTTGGGTGCAGCTGTGAAGGCAGGAGCTT
GCATGCTGGATGGTGGCAATATGTTGGAGACAATCAAGGTGTCACCTCAGACAATGGATGGTATCCTCAA
ATCCATTTTAAAGGTCAAGAAGGCTCTTGGAATGTTCATTTCAGACACCCCTGGTGAAAGGAATCCTTAT
GAAAACATACTCTACAAGATTTGTTTGTCAGGAGATGGATGGCCATATATTGCATCAAGAACCTCAATAA
CAGGAAGGGCCTGGGAAAACACTGTCGTTGATCTGGAATCAGATGGGAAGCCACAGAAAGCTGACAGCAA
CAATTCCAGTAAATCCCTGCAGTCGGCAGGGTTTACCGCTGGGCTTACCTATTCTCAGCTGATGACCCTC
AAGGATGCAATGCTGCAACTTGACCCAAATGCTAAGACCTGGATGGACATTGAAGGAAGACCTGAAGATC
CAGTGGAAATTGCCCTCTATCAACCAAGTTCAGGCTGCTACATACACTTCTTCCGTGAACCTACTGATTT
AAAGCAGTTCAAGCAGGATGCTAAGTACTCACATGGGATTGATGTCACAGACCTCTTCGCTACACAACCG
GGCTTGACCAGTGCTGTCATTGATGCACTCCCCCGGAATATGGTCATTACCTGTCAGGGGTCCGATGACA
TAAGGAAACTCCTTGAATCACAAGGAAGAAAAGACATTAAACTAATTGATATTGCCCTCAGCAAAACTGA
TTCCAGGAAGTATGAAAATGCAGTCTGGGACCAGTATAAAGACTTATGCCACATGCACACAGGTGTCGTT
GTTGAAAAGAAGAAAAGAGGCGGTAAAGAGGAAATAACCCCTCACTGTGCACTAATGGACTGCATCATGT
TTGATGCAGCAGTGTCAGGAGGACTGAACACATCGGTTTTGAGAGCAGTGCTGCCCAGAGATATGGTGTT
CAGAACATCGACACCTAGAGTCGTTCTGTA

Protein Information

Protein Name

nucleoprotein

NCBI Protein GI

23343510

Protein Accession

NP_694869

Protein pI

8.58

Protein Weight

59623.51

Protein Length

569

Protein Sequence

>NP_694869.1 nucleoprotein [Lassa mammarenavirus]
MSASKEIKSFLWTQSLRRELSGYCSNIKLQVVKDAQALLHGLDFSEVSNVQRLMRKERRDDNDLKRLRDL
NQAVNNLVELKSTQQKSILRVGTLTSDDLLILAADLEKLKSKVIRTERPLSAGVYMGNLSSQQLDQRRAL
LNMIGMSGGNQGARAGRDGVVRVWDVKNAELLNNQFGTMPSLTLACLTKQGQVDLNDAVQALTDLGLIYT
AKYPNTSDLDRLTQSHPILNMIDTKKSSLNISGYNFSLGAAVKAGACMLDGGNMLETIKVSPQTMDGILK
SILKVKKALGMFISDTPGERNPYENILYKICLSGDGWPYIASRTSITGRAWENTVVDLESDGKPQKADSN
NSSKSLQSAGFTAGLTYSQLMTLKDAMLQLDPNAKTWMDIEGRPEDPVEIALYQPSSGCYIHFFREPTDL
KQFKQDAKYSHGIDVTDLFATQPGLTSAVIDALPRNMVITCQGSDDIRKLLESQGRKDIKLIDIALSKTD
SRKYENAVWDQYKDLCHMHTGVVVEKKKRGGKEEITPHCALMDCIMFDAAVSGGLNTSVLRAVLPRDMVF
RTSTPRVVL

Epitope Information

IEDB Linear Epitope