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LplA

General Information
Protegen ID 609
Sequence Strain (Species/Organism) Streptococcus pneumoniae R6
VO ID VO_0011194
Taxonomy ID 171101
Molecule Role Protective antigen
Molecule Role Annotation The production of a recombinant vaccine was achieved following PCR amplification of putative lipoate protein ligase (Lpl), with subsequent cloning and expression of the genes in E. coli. The putative lipoate protein ligase (Lpl) , resulted in a reduced CFU titre and a trend towards reduced mortality in an animal sepsis model (Morsczeck et al., 2008).
COG COG0095H, under H: Coenzyme transport and metabolism
References
Morsczeck et al., 2008: Morsczeck C, Prokhorova T, Sigh J, Pfeiffer M, Bille-Nielsen M, Petersen J, Boysen A, Kofoed T, Frimodt-Møller N, Nyborg-Nielsen P, Schrotz-King P. Streptococcus pneumoniae: proteomics of surface proteins for vaccine development. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. 2008; 14(1); 74-81. [PubMed: 18034862].
Gene Information
Gene Name LplA
NCBI Gene ID 934072
Genbank Accession AE007317
Locus Tag spr1047
Gene Starting Position 1041220
Gene Ending Position 1042209
DNA Sequence 
>NC_003098.1:1041220-1042209 Streptococcus pneumoniae R6 chromosome, complete genome
TTTATCCAACTACCGCTTCAGCGATTTCTTCACGGCTAATACCAGCGAAGTAGCGTGTGATATCAATGGT
TTTTAGCGCCTTAAGAACATCTTCGCGTTCGTATTTCACCCCACGAAGGACATCTTCTACTGCAGCAACG
TCTTCAATACCAAAGAAGTCACCATAAATCTTGATGTCTTGGATTTTTGATTCAGTAACGTTAGCAAAGA
CTTCAACCTTACCACTAGTGAATTTGGTTCCACGACGGACGTTAAATTCAGGTGATTTACCATAGTTCCA
GTCCCAAGTTCCAAACTTAGTATCCTTGATGCGATTGATTTCGGCCAATTCTTCTTCTGAAAAGACGTAT
TCAGTCATCTCTGGGTACTCTTTTTTCATGTATTCCAAGAGTAAATCACGGAATTTTTCGACTGTGATTT
TTTTTGGTAATTCATTGATAATATTGGTTACACGGGCACGGACGGATTTCACACCTTTTGATTCAAATTT
ATCTTTTGAAACCTTAAGGGCATTTGCGAGGACTGACAAATCAACGTCAAAGAGCAAGCAACCGTGGTGC
ATGATACGGCCGTTGATATAGGCTTGGGCATTGCCACAGAACTTCTTACCATCAATCTCAAGGTCATTAC
GACCTGTGAACTCAGCTTTAACCCCAAGTTGAGCCAGGGTATTGATAACCGGAGTTGAGAAGCTCTTGAA
GTCAAATGCCTTATTTTCATCTTCTTTGGAGATGATCGTGTAGTTGAGGTTATTTAAATCGTGGTAAACA
GCTCCACCACCACTAATACGGCGAACTACCTCAATACCATTTTCGCGAACATAATCACGGTTGATTTCTT
CGATAGTGTTCTGGTGACGACCAACAATGATAGATGGCTTGTTAATCCAAAGTAGGAAGATTTGATCCTC
ATCCAAAAGGTGTTTAAAGGCGTATTCTTCCAAGGCAATATTAAAAGCAGTGTCATTTGAATGATTGATA
ATGTATTTCA
Protein Information
Protein Name lipoate-protein ligase
NCBI Protein GI 15903091
Protein Accession NP_358641
Protein pI 5.54
Protein Weight 35144.06
Protein Length 329
Protein Note Putative
Protein Sequence 
>NP_358641.1 lipoate-protein ligase [Streptococcus pneumoniae R6]
MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVGRHQNTIEEINRDYVRENGIEVVRRIS
GGGAVYHDLNNLNYTIISKEDENKAFDFKSFSTPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYIN
GRIMHHGCLLFDVDLSVLANALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYP
EMTEYVFSEEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIYGDFF
GIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG
Epitope Information
IEDB Linear Epitope