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collagen binding, ancillary pilus subunit Cne

General Information
Protegen ID 629
Sequence Strain (Species/Organism) Streptococcus equi subsp. equi 4047
VO ID VO_0011213
Taxonomy ID 553482
Other Database IDs CDD:283410
CDD:212461
CDD:273478
Molecule Role Protective antigen
Molecule Role Annotation Mice were immunised i.n. with collagen-binding protein (CNE) and were subsequently subjected to infection with S. equi subsp. equi. A control group of mice was given only adjuvant. The CNE vaccinated group had a delayed onset of infection compared to the control group. Immunization with CNE led to significant protection from infection (Flock et al., 2006).
The antigens used were EAG (alpha2-macroglobulin, albumin, and IgG-binding protein), CNE (a collagen-binding protein), and SclC (a collagen-like protein). The vaccinated horses showed significantly reduced bacterial growth (p=0.02).(Waller et al., 2007)
Related Vaccines(s) S. equi Subunit CNE Protein Vaccine
References
Flock et al., 2006: Flock M, Karlström A, Lannergård J, Guss B, Flock JI. Protective effect of vaccination with recombinant proteins from Streptococcus equi subspecies equi in a strangles model in the mouse. Vaccine. 2006; 24(19); 4144-4151. [PubMed: 16580099].
Waller et al., 2007: Waller A, Flock M, Smith K, Robinson C, Mitchell Z, Karlström A, Lannergård J, Bergman R, Guss B, Flock JI. Vaccination of horses against strangles using recombinant antigens from Streptococcus equi. Vaccine. 2007; 25(18); 3629-3635. [PubMed: 17321016].
Gene Information
Gene Name collagen binding, ancillary pilus subunit Cne
NCBI Gene ID 7695900
Genbank Accession FM204883
Locus Tag SEQ_0935
Gene Starting Position 914185
Gene Ending Position 916158
DNA Sequence 
>NC_012471.1:914185-916158 Streptococcus equi subsp. equi 4047, complete genome
TTTGAAACAACTGACAAAGATCGTTAGTGTGGTCTTGTTGCTGGTCTTTACCCTTAGTGCTAGCCTGCAC
AAGGTTCGGGCAACTAATCTTAGTGACAACATCACATCATTGACGGTTGCTTCTTCATCACTCCGAGATG
GAGAGAGAACGACGGTAAAGGTTGCGTTTGATGACAAAAAACAGAAAATCAAGGCAGGGGATACGATAGA
GGTCACCTGGCCTACAAGTGGTAATGTCTACATTCAGGGCTTTAATAAAACCATACCGCTTAATATTAGA
GGGGTAGATGTTGGTACCTTGGAGGTCACGCTAGACAAGGCTGTTTTCACATTCAATCAAAATATTGAAA
CAATGCATGATGTCTCTGGTTGGGGAGAGTTTGATATTACTGTTAGAAATGTGACACAAACCACCGCTGA
AACATCAGGAACGACCACAGTAAAGGTAGGCAATCGCACTGCTACTATCACTGTTACTAAGCCTGAGGCA
GGCACTGGTACCAGCTCATTTTATTATAAGACTGGTGATATGCAGCCCAATGATACTGAGCGTGTGAGAT
GGTTCCTGCTGATTAACAACAACAAGGAATGGGTGGCCAATACTGTTACAGTCGAAGACGATATTCAAGG
TGGTCAAACCTTGGATATGAGCAGCTTTGACATCACCGTATCTGGTTATCGTAACGAGCGCTTCGTTGGG
GAAAACGCTCTGACAGAGTTTCATACAACATTTCCAAATTCTGTCATTACGGCAACAGATAATCACATTA
GTGTGCGGTTAGATCAATATGATGCCTCACAAAACACTGTCAACATTGCTTATAAGACAAAGATAACGGA
CTTTGACCAAAAAGAATTTGCCAACAACAGTAAAATCTGGTACCAGATTTTATACAAGGATCAGGTATCG
GGTCAAGAGTCAAACCACCAAGTAGCCAATATCAATGCTAACGGCGGGGTTGATGGCAGTCGCTATACCA
GCTTTACTGTCAAGAAAATTTGGAATGACAAGGAAAATCAAGACGGTAAGCGTCCAAAGACTATTACTGT
TCAGCTTTACGCCAATGATCAGAAAGTTAATGATAAGACCATTGAATTGAGTGATACTAATAGCTGGCAA
GCAAGTTTTGGTAAGCTGGATAAGTATGACAGTCAGAACCAAAAAATTACCTACAGTGTCAAGGAAGTGA
TGGTTCCTGTTGGCTACCAATCGCAGGTTGAGGGGGATAGTGGAGTAGGATTTACCATTACCAACACCTA
TACACCAGAGGTCATTAGCATTACCGGTCAAAAAACTTGGGACGACAGGGAAAACCAAGACGGTAAACGT
CCTAAGGAGATTACGGTTCGTTTATTGGCAAATGACGCTGCAACTGACAAGGTAGCAACTGCTTCAGAGC
AAACCGGCTGGAAGTATACATTTACCAATCTACCGAAATACAAAGATGGTAAACAGATCACCTACACGAT
CCAAGAGGACCCTGTGGCAGATTACACCACAACCATTCAGGGATTTGATATTACCAATCATCATGAGGTA
GCCTTGACCAGCCTAAAGGTCATCAAGGTTTGGAATGATAAGGACGATTATTACCATAAACGTCCCAAGG
AGATTACCATTTTGCTAAAGGCAGATGGCAAGGTGATTCGTGAACATCAGATGACACCGGATCAGCAAGG
AAAATGGGAATACACCTTTGACCAGCTGCCGGTCTATCAGACAGGCAAGAAAATCAGCTACAGCATTGAG
GAAAAACAGGTTGCTGGCTATCAAGCCCCTGTCTATGAGGTTGATGAAGGCTTGAAGCAGGTCACTGTAA
CCAACACCCTTAACCCAAGCTACAAGCTGCCTGACACCGGAGGACAAGGAGTGAAATGGTACCTGTTAAT
CGGTGGCGGTTTTATCATCGTCGCAATCCTTGTACTGATCAGCCTTTATCAAAAACACAAGCGCCATAAC
ATGTCAAAACCATA
Protein Information
Protein Name collagen binding, ancillary pilus subunit Cne
NCBI Protein GI 225870316
Protein Accession YP_002746263
Protein pI 6.61
Protein Weight 68135.68
Protein Length 657
Protein Note Collagen binding domain; pfam05737
Protein Sequence 
>YP_002746263.1 collagen binding, ancillary pilus subunit Cne [Streptococcus equi subsp. equi 4047]
MKQLTKIVSVVLLLVFTLSASLHKVRATNLSDNITSLTVASSSLRDGERTTVKVAFDDKKQKIKAGDTIE
VTWPTSGNVYIQGFNKTIPLNIRGVDVGTLEVTLDKAVFTFNQNIETMHDVSGWGEFDITVRNVTQTTAE
TSGTTTVKVGNRTATITVTKPEAGTGTSSFYYKTGDMQPNDTERVRWFLLINNNKEWVANTVTVEDDIQG
GQTLDMSSFDITVSGYRNERFVGENALTEFHTTFPNSVITATDNHISVRLDQYDASQNTVNIAYKTKITD
FDQKEFANNSKIWYQILYKDQVSGQESNHQVANINANGGVDGSRYTSFTVKKIWNDKENQDGKRPKTITV
QLYANDQKVNDKTIELSDTNSWQASFGKLDKYDSQNQKITYSVKEVMVPVGYQSQVEGDSGVGFTITNTY
TPEVISITGQKTWDDRENQDGKRPKEITVRLLANDAATDKVATASEQTGWKYTFTNLPKYKDGKQITYTI
QEDPVADYTTTIQGFDITNHHEVALTSLKVIKVWNDKDDYYHKRPKEITILLKADGKVIREHQMTPDQQG
KWEYTFDQLPVYQTGKKISYSIEEKQVAGYQAPVYEVDEGLKQVTVTNTLNPSYKLPDTGGQGVKWYLLI
GGGFIIVAILVLISLYQKHKRHNMSKP
Epitope Information
IEDB Linear Epitope