VIOLIN: Vaccine Investigation and Online Information Network

Sfs

General Information

Protegen ID

631

Sequence Strain (Species/Organism)

Streptococcus equi subsp. equi 4047

VO ID

VO_0011215

Taxonomy ID

553482

Molecule Role

Protective antigen

Molecule Role Annotation

Parts of the three recombinant proteins (FNZ (cell surface-bound fibronectin binding protein), SFS (secreted fibronectin binding protein), and EAG (alpha2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein)) were used to immunize mice, either subcutaneously or intranasally, prior to nasal challenge with S. equi subsp. equi. The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin. It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) (Flock et al., 2004).

Related Vaccines(s)

S. equi Subunit EAG, FNZ and SFS Protein Vaccine

References

Flock et al., 2004: Flock M, Jacobsson K, Frykberg L, Hirst TR, Franklin A, Guss B, Flock JI. Recombinant Streptococcus equi proteins protect mice in challenge experiments and induce immune response in horses. Infection and immunity. 2004; 72(6); 3228-3236. [PubMed: 15155624].

Gene Information

Gene Name

Sfs

NCBI Gene ID

7696331

Genbank Accession

FM204883

Locus Tag

SEQ_0466

Gene Starting Position

452509

Gene Ending Position

453624

DNA Sequence

>NC_012471.1:452509-453624 Streptococcus equi subsp. equi 4047, complete genome
TATGAGAAAAACAGAAGGACGTTTTCGCACATGGAAGTCCAAAAAACAATGGCTATTTGCCGGTGCAGTG
GTGACCAGCTTGCTGCTGGGAGCTGCACTTGTCTTTGGAGGTTTATTAGGAAGTCTTGGTGGCTCATCCC
ATCAAGCGAGGCCAAAGGAGCAGCCAGTCAGCTCGATTGGAGATGACGATAAGTCGCACAAGAGCTCATC
AGATTCAATGGTCAGTCGGCCACCGAAAAAGGATAACTTGCAGCCTAAGCCTTCAGATCAGCCTACTAAT
CATCAGCATCAGGCTACTAGCCCGTCCCAGCCGACAGCAAAGAGCTCAGGTCATCATGGGAATCAACCTC
AGTCTCTCAGTGTGAACAGCCAAGGAAATAGTAGTGGACAGGCCTCAGAGCCTCAGGCTATTCCTAATCA
AGGGCCTTCCCAGCCACTGGGGCTGAGAGGAGGTAACAGCTCTGGTTCAGGTCATCACCATCAGCCACAA
GGAAAGCCACAGCACTTAGATCTAGGTAAGGATAATTCTAGCCCGCAGCCTCAACCAAAGCCTCAGGGCA
ATAGTCCCAAGCTGCCAGAAAAAGGCTTGAATGGTGAAAATCAGAAGGAACCGGAGCAAGGTGAACGAGG
TGAGGCTGGTCCCCCACTTTCAGGGTTGAGTGGTAATAATCAAGGCCGTCCTTCGCTTCCAGGCTTGAAT
GGTGAGAATCAGAAGGAACCAGAGCAAGGTGAACGAGGTGAAGCCGGTCCCCCATCAACTCCGAATTTAG
AAGGAAATAATCGTAAAAATCCTTTAAAAGGATTAGATGGAGAGAATAAGCCAAAGGAAGATTTAGACGG
TAAAGGCTTAAGCGGAGAAAATGATGAATCACCAAAACTTAAAGACGAACACCCCTACAATCATGGTCGT
CGTGATGGTTACCGAGTTGGCTATGAGGATGGATATGGTGGCAAAAAGCACAAAGGAGATTATCCTAAGC
GGTTTGATGAGTCTAGTCCAAAGGAATATAATGACTATAGTCAAGGGTATAATGATAATTATGGAAATGG
CTATTTAGATGGTCTAGCTGATAGAGGTGGTAAGAGAGGATACGGCTATTCTTACAATCCCGACTA

Protein Information

Protein Name

fibronectin-binding protein Sfs

NCBI Protein GI

225869898

Protein Accession

YP_002745845

Protein pI

8.97

Protein Weight

39068.51

Protein Length

371

Protein Note

Previously sequenced as Streptococcus equi fibronectin-binding protein Sfs UniProt: AF136451 (371 aa" target="_blank">Q9X6F4 (EMBL: AF136451 (371 aa) fasta scores: E()=1.8e-153, 100.000% id in 371 aa

Protein Sequence

>YP_002745845.1 fibronectin-binding protein Sfs [Streptococcus equi subsp. equi 4047]
MRKTEGRFRTWKSKKQWLFAGAVVTSLLLGAALVFGGLLGSLGGSSHQARPKEQPVSSIGDDDKSHKSSS
DSMVSRPPKKDNLQPKPSDQPTNHQHQATSPSQPTAKSSGHHGNQPQSLSVNSQGNSSGQASEPQAIPNQ
GPSQPLGLRGGNSSGSGHHHQPQGKPQHLDLGKDNSSPQPQPKPQGNSPKLPEKGLNGENQKEPEQGERG
EAGPPLSGLSGNNQGRPSLPGLNGENQKEPEQGERGEAGPPSTPNLEGNNRKNPLKGLDGENKPKEDLDG
KGLSGENDESPKLKDEHPYNHGRRDGYRVGYEDGYGGKKHKGDYPKRFDESSPKEYNDYSQGYNDNYGNG
YLDGLADRGGKRGYGYSYNPD

Epitope Information

IEDB Linear Epitope