VIOLIN: Vaccine Investigation and Online Information Network

CS from P. berghei str. ANKA

General Information

Protegen ID

633

Sequence Strain (Species/Organism)

Plasmodium berghei ANKA

VO ID

VO_0011217

Taxonomy ID

5823

Molecule Role

Protective antigen

Molecule Role Annotation

Researchers produced a prototypic malaria vaccine based on a highly versatile self-assembling polypeptide nanoparticle (SAPN) platform that can repetitively display antigenic epitopes. Researchers used this platform to display a tandem repeat of the B cell immunodominant repeat epitope (DPPPPNPN)(2)D of the malaria parasite Plasmodium berghei circumsporozoite (CS) protein. Administered in saline, without the need for a heterologous adjuvant, the SAPN construct P4c-Mal conferred a long-lived, protective immune response to mice with a broad range of genetically distinct immune backgrounds including the H-2(b), H-2(d), and H-2(k) alleles. Mice were protected against an initial challenge of parasites up to 6 mo after the last immunization or for up to 15 mo against a second challenge after an initial challenge of parasites had successfully been cleared (Kaba et al., 2009).

Related Vaccines(s)

P. berghei CS Protein Subunit Vaccine , P. berghei DNA vaccine CSP-3p28 , P. berghei DNA vaccine encoding PbCSP

References

Kaba et al., 2009: Kaba SA, Brando C, Guo Q, Mittelholzer C, Raman S, Tropel D, Aebi U, Burkhard P, Lanar DE. A nonadjuvanted polypeptide nanoparticle vaccine confers long-lasting protection against rodent malaria. Journal of immunology (Baltimore, Md. : 1950). 2009; 183(11); 7268-7277. [PubMed: 19915055].

Gene Information

Gene Name

CS from P. berghei str. ANKA

NCBI Gene ID

3428738

Genbank Accession

LK023119

Chromosome No

Locus Tag

PBANKA_040320

Gene Starting Position

126554

Gene Ending Position

127576

DNA Sequence

>NC_036162.1:126554-127576 Plasmodium berghei ANKA genome assembly PBANKA01, chromosome : 4
TTTAATTAAAGAATACTAATACTAATAATATTACAAATCCTAATGAATTGCTTACAATATTAAATATACT
TGAACATTTATCCATTTTACAAATTTCAGTATCAATATCTTCTAAGGTCAAATCTTCTGCTTTCTTATTT
GAACCTTTTCGTTTTCTAACTCTTATACCAGAACCACATGTTACGTTACATTGAGACCATTCCTCTGTGA
TACTATCCCTGATCTGTTTAACAAATTCTAGTATTTTTTCCGCGCTTGGGATATAAGAATCGTCATTATT
ATTATTTTTGTTATTGTTATTACCACCTGGCTGTGGTTGTGGCTGTGGTCGTGGCTGTGGTTGTGGCTGT
GGCTGTGGTTGTGGCTGTGGCTGTGGTTGTGGCTGCGGCCGTGGCTGTGGTTGTGGATTGTTATTTCCTT
GTGGTGGTGCTGGGTCATTTGGGTTTGGTGGTGGTGGGTCATTTGCGTTTGGTGGTGCTGGGTCATTTGG
GTTTGGTGGTGGTGGGTCATTTGCGTTTGGTGGTGCTGGGTCATTTGCGTTTGGTGGTGCTGGGTCATTT
GCGTTTGGTGGTGCTGGGTCATTTGCGTTTGGTGGTGCTGGGTCATTTGCGTTTGGTGGTGGTGGGTCAT
TTGCGTTTGGTGGTGGTGGGTCATTTGGGTTTGGTGGTGGTGGGTCATTTGGGTTTGGTGGTGGTGGGTC
ATTTGGGTTTGGTGGTGGTGGGTCATTTGGGTTTGGTGGTGGTGGTGGTTGTTTCAATTTATTATTACGC
TCTATTTTTTCGTTTTTTTTCTCATTTTTTTTTCCTTCGGGAGCATCGGCAAGTAATCTGTTGACTGTAT
TTCGATTGTATATTTTTCCATTCTTAGAGTTAAGCACGTGATACAATTTATTATCATTTCCTTCATTGTA
ACATAGCTCGTTTAAGTTCCTTTGGGCTTGGATGCTTTTATTTTGTCCATATCCTGGAAGTAGAGAATTA
ACTAATAAAAGTGACGCTACAACTAAAATGGTACACTTCTTCA

Protein Information

NCBI Protein GI

AAA29577.1

Protein Accession

XP_022712148

Protein pI

5.2

Protein Weight

35858.22

Protein Length

340

Protein Sequence

>AAA29577.1 circumsporozoite protein [Plasmodium berghei ANKA]
MKKCTILVVASLLLVNSLLPGYGQNKIIQAQRNLNELCYNEGNDNKLYHVLNSKNGKIYNRNTVNRLLPM
LRRKKNEKKNEKIERNNKLKQPPPPPNPNDPPPPNPNDPPPPNPNDPPPPNPNDPPPPNANDPPPPNAND
PAPPNANDPAPPNANDPAPPNANDPAPPNANDPAPPNANDPAPPNANDPPPPNPNDPAPPQGNNNPQPQP
RPQPQPQPQPQPQPQPQPQPRPQPQPQPGGNNNNKNNNNDDSYIPSAEKILEFVKQIRDSITEEWSQCNV
TCGSGIRVRKRKGSNKKAEDLTLEDIDTEICKMDKCSSIFNIVSNSLGFVILLVLVFFN

Epitope Information

IEDB Linear Epitope