VIOLIN: Vaccine Investigation and Online Information Network

Kbeta

General Information

Protegen ID

649

Sequence Strain (Species/Organism)

Plasmodium falciparum 3D7

VO ID

VO_0011233

Taxonomy ID

36329

Molecule Role

Protective antigen

Molecule Role Annotation

Two large fragments of PfKbeta representing the N- and C-terminal halves were expressed in E. coli. The recombinant proteins were highly immunogenic in mice, and also found to be the target for immune response in natural infections of Plasmodium spp. Immunization with recombinant PfKbeta fragments was partially protective against a heterologous challenge infection in mice (Mohmmed et al., 2005).

References

Mohmmed et al., 2005: Mohmmed A, Kishore S, Patra KP, Dasaradhi PV, Malhotra P, Chauhan VS. Identification of karyopherin beta as an immunogenic antigen of the malaria parasite using immune mice and human sera. Parasite immunology. 2005; 27(5); 197-203. [PubMed: 15987343].

Gene Information

Gene Name

Kbeta

NCBI Gene ID

813054

Genbank Accession

AL844504

Chromosome No

Locus Tag

PF3D7_0524000

Gene Starting Position

998752

Gene Ending Position

1002123

DNA Sequence

>NC_004326.2:998752-1002123 Plasmodium falciparum 3D7 chromosome 5
AATGGATAAAATTGTAGAAGTTATTGAAGGATTAAGCAGCTCTGAGAGTCATATAAGAAATGAATGTGAA
AATACGTTAAATTATTATAAAAAGAATGATTTAAATAATACAGTATTATCTATATTGAAATTATTAAAAA
CCCATAAAGATAGTCAAGTTCGATTACAATGTGCTATATTAATTAGGAATATATTTCGTGTGTATATAAA
GTCCACACATGTGGATGTGGAAGAAAAAGAAAAAAATGAGAATTCGATTGGAAATGCAGAAGAAGAGAAT
TATTGGGTATTATTACCTGATAATTTAAAGAATATTGTAAAATCTGAATTGATTAGTAATATAGGAACTG
AAACAGATAAGATGGTTCGAAGTAATATATGTAATAATATTATAGATTTATCATCAAAATTATTATTACA
TAATCAATGGCCTGAGTTATTATCAGTAACATTTGAATTTTGTAATTCTAATAACGTTGATGTATTAATA
AGTGGATATAAAATATTAGGTGGTATCTTAAGTTGTATTCCAGATGAATTAGATGGAAAACAAGAAATAA
TATCTTCCATATGTATGAAAGGTTTAAATTCATCAAATGTACAAGTACGTGGAGAATGTATTAATTTAAT
ATCATGTATTGTTGAAGATAATAGTTCATCCTTAGTTAAGAGTGTACATGGTTGTATCCCATTAATATTA
CAATCATTAAGTTTGATGGCAAAGAATAGTAGTTCAGATATTGCTGTTCTAGAAGAATGTGAGAAAGTAC
TTCAATCTATTGGTAAAATGATAGATTATAATGCTAAATTTTTTACTAAGCATATAACAAGTTTGTGTGA
TATATTATTTAGTATTTGTATGAAAGATGAAAATGAATTGAACTATGATTTTGATAATAGCTTGAAGTCC
CTAAGTATTGAAGCTTTAGTTACCATTCCAGAACGTCGACCAAAAATGGCTTTATCTGTACCTCATTTTG
TAGATAAGATTATACATTTGTCTATGTTATTTATGCTTGACATTAATAATGATTGTTTTAATGAATGGAT
GAATTCTATAAAAGAAGGTAAAGACGATAGTCAGGAGTTATATGACATAGGTGAAGAGTCCTTAGATCGT
GTTGGTAAAGCATTTAGTGAGTTAGAAGAAGCTGAATTTATTCATATTCTTTTTAATAAAGTATCAGAAT
TTTTAATGAAAAATACTTGGGAACATAAATATGTCGGTATTATGGCTATTGCACAAACTATAGAATATTT
ACCAGAAGATGAAATTGAAGAACAATTAGAACATGTTATAAAAATGTTGTTACAAATATTAGTTGATCAA
GATGTTAGAGTACGTTATGCAGCATGTCAAGCCATTGGACAAATATCTTTAGATCATCAACCATATGTAC
AGAAAGAATTTTTTTCAGAAATTTTATCAGCATTAATTAATACCATGAACGATGTACATTTAAGAGTTCA
ATCACATGCTACAGCAGCTTTTGTAAATTATGCAGAAGAATTAGATAAAATGGCTTTATTACCATATGCA
GATATTATTATAGATATTTTATTACAAAAATTGAATTCTTCAAATTATTTATTAGTAAGAGAACAAGCTG
TTACTGCTATAGCGGTTATTGCAGGTGTTATTGAAGAAGACTTTTTAAAATATTATTCTACTGTTGTACC
TATGATGAAAGATATTATTCAAAAGGCAGTTTCTGAAGAAGAAAGAACATGCAGAGGTAAAGCCATTGAA
TGTATTTCAATTATTGGTTTATCGGTTGGTAAAGATATTTTTATTGAAGATGCTAAAGAATGTATGAATG
CATTATTACAAATTAGCAGTACAAAAATGGATCCTGATGATACTGTAAAAGAATATATTCAAGAAGCTAT
TGGTCGTATATGTAGAGCATTAGGTAATGACTTTTATCCATATCTAAGTAGTATAGTACCGACCATATTA
TCTGTACTCTCCGTTTTACCAAAACCATTAACAGATGATGAAGAAGATCTAACCATAACTATGGTATCTA
ATGGACAATATGTAGGATTGAAAACATCCTTATTAGAAGATCAAGAAAAAGCTTTAGATTTATTAATTAT
TATTATTGAAGTATTAAAAGAAAATTATAAGGATTATATTCAAGCAACAGCTACAGCTGTGTTGCCAATG
TTAAATTATGAATTGTCAGATGAAATTAAACAAAAGGCATTAACAGCTGTTAGTGAATTAATTGAATCAG
CAAGAATATTATCTGAAAAAACGGATAATGATAAATCCATGTTACTAGCCATATTAACAGCAGCAGCAGA
AAAAGTATTAAAAAGTCTATTAGAAACAAAATTAGATGACAATTATGAATATATATTAGATGTTATGATA
ATCGAATCCCATGGATTATATATGTGCTTACAAAAAGCGGGATCGAATGTATTGCCAGAAAATACATTAA
AATTATTTTTTAATCAAATCTTTGCCCTATTACAATATTCAACAGATAGAAGGGTAGTATATAATCAAAA
AAAAAATAATGATGATGTAGATGAAGATGAATTATTAATAATTGATAGAGAAGAGGAATTAGAACAGAAT
TATCGAACAAATTTATTAGATATCTTAGGAGTATTAATTAAATATCACCCAACACAATTCTTAAATACAT
GTTGTGAATTATGTATTGGATTTATTAATAATTATATGAATTCTCCTAATTCAGAAGATGTAGCATTAGC
ATTATATGTATGTGATGATTTGTTAGAATTCTTACAAGAAAAGAGTGTAAACCTATGGGACTTTTTCATG
AACCCATTATTATTAAATATTAATCATGCAGATGATAAAGTAAAACAAGCGGCATGTTATGGTGTTATAC
AAGCAACCAAAATTGAAGCATTTGGAAAGTATGCAAATATTGCAGTAGAATATTTATTAAAATTAGTACA
TGAAAGTACATCAAATAAAAAACCAAAAGAATATATCTCAGCTATTGATAATGCTATAGCAGCATTAGGT
GATGTTGTTCTTATGCATACATCAAAATTTAATAATGCAGAAGATCTCATAAAAGTATGGTTAAATCATT
TACCAATAAAAGAAGATGATGCAGAAGGTAGAAGAGTACATAAAAATCTTATAGATTTAGTTTCACAAAA
CCATCCTCTCTTGTTCGGAAAAGATAATTCTAATACAGCAAAAATTATTGAAATATTCTTAACAATATAT
GAAACTGATTTTTCAGATACTGACTGTAACAAAAAAATCTCAACACTTATCAATTCATTGGATAAATCAT
ATTTAAATAATTTAGCTTCTTCAACTTTGTCACATAAGCAAGCCAAAAAATTGAACAACATTTTGAATCC
AAACAGAAAATA

Protein Information

NCBI Protein GI

124506397

Protein Accession

XP_001351796

Protein pI

4.54

Protein Weight

120404.67

Protein Length

1123

Protein Sequence

>XP_001351796.1 karyopherin beta [Plasmodium falciparum 3D7]
MDKIVEVIEGLSSSESHIRNECENTLNYYKKNDLNNTVLSILKLLKTHKDSQVRLQCAILIRNIFRVYIK
STHVDVEEKEKNENSIGNAEEENYWVLLPDNLKNIVKSELISNIGTETDKMVRSNICNNIIDLSSKLLLH
NQWPELLSVTFEFCNSNNVDVLISGYKILGGILSCIPDELDGKQEIISSICMKGLNSSNVQVRGECINLI
SCIVEDNSSSLVKSVHGCIPLILQSLSLMAKNSSSDIAVLEECEKVLQSIGKMIDYNAKFFTKHITSLCD
ILFSICMKDENELNYDFDNSLKSLSIEALVTIPERRPKMALSVPHFVDKIIHLSMLFMLDINNDCFNEWM
NSIKEGKDDSQELYDIGEESLDRVGKAFSELEEAEFIHILFNKVSEFLMKNTWEHKYVGIMAIAQTIEYL
PEDEIEEQLEHVIKMLLQILVDQDVRVRYAACQAIGQISLDHQPYVQKEFFSEILSALINTMNDVHLRVQ
SHATAAFVNYAEELDKMALLPYADIIIDILLQKLNSSNYLLVREQAVTAIAVIAGVIEEDFLKYYSTVVP
MMKDIIQKAVSEEERTCRGKAIECISIIGLSVGKDIFIEDAKECMNALLQISSTKMDPDDTVKEYIQEAI
GRICRALGNDFYPYLSSIVPTILSVLSVLPKPLTDDEEDLTITMVSNGQYVGLKTSLLEDQEKALDLLII
IIEVLKENYKDYIQATATAVLPMLNYELSDEIKQKALTAVSELIESARILSEKTDNDKSMLLAILTAAAE
KVLKSLLETKLDDNYEYILDVMIIESHGLYMCLQKAGSNVLPENTLKLFFNQIFALLQYSTDRRVVYNQK
KNNDDVDEDELLIIDREEELEQNYRTNLLDILGVLIKYHPTQFLNTCCELCIGFINNYMNSPNSEDVALA
LYVCDDLLEFLQEKSVNLWDFFMNPLLLNINHADDKVKQAACYGVIQATKIEAFGKYANIAVEYLLKLVH
ESTSNKKPKEYISAIDNAIAALGDVVLMHTSKFNNAEDLIKVWLNHLPIKEDDAEGRRVHKNLIDLVSQN
HPLLFGKDNSNTAKIIEIFLTIYETDFSDTDCNKKISTLINSLDKSYLNNLASSTLSHKQAKKLNNILNP
NRK

Epitope Information

IEDB Linear Epitope