VIOLIN: Vaccine Investigation and Online Information Network

MAGEA3

General Information

Protegen ID

708

Sequence Strain (Species/Organism)

Homo sapiens

Taxonomy ID

9606

Molecule Role

Protective antigen

Molecule Role Annotation

Vaccine- and tumor-specific immune responses of 10 melanoma patients treated with autologous GMLs expressing the cancer germline gene MAGE-A3 were analyzed. Three of 10 patients treated with MAGE-A3-GML showed an increase of circulating anti-MAGE-A3 T cells, and developed skin delayed-type hypersensitivity to MAGE-A3. Interestingly, in 2 of these patients, with progressive and measurable tumors at study entry, anti-MAGE-A3 T cells were detected not only in the blood but also within tumors resected after vaccination. These results demonstrate that the infusion of MAGE-A3-GML elicits antitumor T cells, which are capable of trafficking to inflamed tissues and of infiltrating tumors (Fontana et al., 2009).

Related Vaccines(s)

4-peptide melanoma vaccine , Allogeneic GM-CSF-Based Myeloma Cell Vaccine , Autologous Cancer Testis Antigen Specific Dendritic Cell Vaccine , Autologous CT7/MAGE-A3/WT1 mRNA-Electroporated Langerhans-Type Dendritic Cells , Autologous Dendritic Cell-Allogeneic Melanoma Tumor Cell Lysate Vaccine , Autologous TriMix-DC Melanoma Vaccine , Autologous-Cell Leukemia Vaccine , EP-2101 Vaccine , HLA-A1-Binding MAGE-1/MAGE-3 Multipeptide-Pulsed Autologous Dendritic Cell Vaccine , Immunotherapeutic GSK1572932A , MAGE-3.A1 Peptide Vaccine , MAGE-A1/MAGE-A3/NY-ESO-1 Peptides Vaccine , MAGE-A3 Peptide Vaccine , MAGE-A3-expressing Adenovirus Type 5 Vaccine , MAGE-A3/HPV 16 Peptide Vaccine , MART-1/gp100/Tyrosinase/MAGE-A3 Peptides-loaded Irradiated Allogeneic Plasmacytoid Dendritic Cells Vaccine , Melan-A/MAGE-3.DP4 Peptide Vaccine , MELITAC 12.1 Peptide Vaccine , Multi-epitope melanoma peptide vaccine , NA-17/MAGE-3.A2/NY-ESO-1 Peptide Vaccine , NA17.A2 Peptide Vaccine , NY-ESO-1(157-165) Peptide-pulsed Autologous Dendritic Cell Vaccine , NY-ESO-1/PRAME/MAGE-A3/WT-1 Peptide Vaccine

References

Fontana et al., 2009: Fontana R, Bregni M, Cipponi A, Raccosta L, Rainelli C, Maggioni D, Lunghi F, Ciceri F, Mukenge S, Doglioni C, Colau D, Coulie PG, Bordignon C, Traversari C, Russo V. Peripheral blood lymphocytes genetically modified to express the self/tumor antigen MAGE-A3 induce antitumor immune responses in cancer patients. Blood. 2009; 113(8); 1651-1660. [PubMed: 19074732].

Gene Information

Gene Name

MAGEA3

NCBI Gene ID

4102

Genbank Accession

AC244102

Chromosome No

Gene Starting Position

152698741

Gene Ending Position

152702346

DNA Sequence

>NC_000023.11:152698741-152702346 Homo sapiens chromosome X, GRCh38.p12 Primary Assembly
CAGAAGACCGCGAGATTCTCGCCCTGAGCAACGAGCGACGGCCTGACGTCGGCGGAGGGAAGCCGGCCCA
GGCTCGGTGAGGAGGCAAGGTAAGACGCTGAGGGAGGACTGAGGCGGGCCTCACCTCAGACAGAGGGCCT
CAAATAATCCAGTGCTGCCTCTGCTGCCGGGCCTGGGCCACCCCGCAGGGGAAGACTTCCAGGCTGGGTC
GCCACTACCTCACCCCGCCGACCCCCGCCGCTTTAGCCACGGGGAACTCTGGGGACAGAGCTTAATGTGG
CCAGGGCAGGGCTGGTTAGAAGAGGTCAGGGCCCACGCTGTGGCAGGAATCAAGGTCAGGACCCCGAGAG
GGAACTGAGGGCAGCCTAACCACCACCCTCACCACCATTCCCGTCCCCCAACACCAACCCCACCCCCATC
CCCCATTCCCCATTCCCATCCCCACCCCCACCCCTATCCTGGCAGAATCCGGGCTTTGCCCCTGGTATCA
AGTCACGGAAGCTCCGGGAATGGCGGCCAGGCACGTGAGTCCTGAGGTTCACATCTACGGCTAAGGGAGG
GAAGGGGTTCGGTATCGCGAGTATGGCCGTTGGGAGGCAGCGAAAGGGCCCAGGCCCTCCTGGAAGACAG
TGGAGTCCTGAGGGGACCCAGCATGCCAGGACAGGGGGCCCACTGTACCCCTGTCTCAAACCGAGGCACC
TTTTCATTCGGCTACGGGAATCCTAGGGATGCAGACCCACTTCAGCAGGGGGTTGGGGCCCAGCCCTGCG
AGGAGTCATGGGGAGGAAGAAGAGGGAGGACTGAGGGGACCTTGGAGTCCAGATCAGTGGCAACCTTGGG
CTGGGGGATGCTGGGCACAGTGGCCAAATGTGCTCTGTGCTCATTGCGCCTTCAGGGTGACCAGAGAGTT
GAGGGCTGTGGTCTGAAGAGTGGGACTTCAGGTCAGCAGAGGGAGGAATCCCAGGATCTGCAGGGCCCAA
GGTGTACCCCCAAGGGGCCCCTATGTGGTGGACAGATGCAGTGGTCCTAGGATCTGCCAAGCATCCAGGT
GAAGAGACTGAGGGAGGATTGAGGGTACCCCTGGGACAGAATGCGGACTGGGGGCCCCATAAAAATCTGC
CCTGCTCCTGCTGTTACCTCAGAGAGCCTGGGCAGGGCTGTCAGCTGAGGTCCCTCCATTATCCTAGGAT
CACTGATGTCAGGGAAGGGGAAGCCTTGGTCTGAGGGGGCTGCACTCAGGGCAGTAGAGGGAGGCTCTCA
GACCCTACTAGGAGTGGAGGTGAGGACCAAGCAGTCTCCTCACCCAGGGTACATGGACTTCAATAAATTT
GGACATCTCTCGTTGTCCTTTCCGGGAGGACCTGGGAATGTATGGCCAGATGTGGGTCCCCTCATGTTTT
TCTGTACCATATCAGGTATGTGAGTTCTTGACATGAGAGATTCTCAGGCCAGCAGAAGGGAGGGATTAGG
CCCTATAAGGAGAAAGGTGAGGGCCCTGAGTGAGCACAGAGGGGATCCTCCACCCCAGTAGAGTGGGGAC
CTCACAGAGTCTGGCCAACCCTCCTGACAGTTCTGGGAATCCGTGGCTGCGTTTGCTGTCTGCACATTGG
GGGCCCGTGGATTCCTCTCCCAGGAATCAGGAGCTCCAGGAACAAGGCAGTGAGGACTTGGTCTGAGGCA
GTGTCCTCAGGTCACAGAGTAGAGGGGGCTCAGATAGTGCCAACGGTGAAGGTTTGCCTTGGATTCAAAC
CAAGGGCCCCACCTGCCCCAGAACACATGGACTCCAGAGCGCCTGGCCTCACCCTCAATACTTTCAGTCC
TGCAGCCTCAGCATGCGCTGGCCGGATGTACCCTGAGGTGCCCTCTCACTTCCTCCTTCAGGTTCTGAGG
GGACAGGCTGACCTGGAGGACCAGAGGCCCCCGGAGGAGCACTGAAGGAGAAGATCTGTAAGTAAGCCTT
TGTTAGAGCCTCCAAGGTTCCATTCAGTACTCAGCTGAGGTCTCTCACATGCTCCCTCTCTCCCCAGGCC
AGTGGGTCTCCATTGCCCAGCTCCTGCCCACACTCCCGCCTGTTGCCCTGACCAGAGTCATCATGCCTCT
TGAGCAGAGGAGTCAGCACTGCAAGCCTGAAGAAGGCCTTGAGGCCCGAGGAGAGGCCCTGGGCCTGGTG
GGTGCGCAGGCTCCTGCTACTGAGGAGCAGGAGGCTGCCTCCTCCTCTTCTACTCTAGTTGAAGTCACCC
TGGGGGAGGTGCCTGCTGCCGAGTCACCAGATCCTCCCCAGAGTCCTCAGGGAGCCTCCAGCCTCCCCAC
TACCATGAACTACCCTCTCTGGAGCCAATCCTATGAGGACTCCAGCAACCAAGAAGAGGAGGGGCCAAGC
ACCTTCCCTGACCTGGAGTCCGAGTTCCAAGCAGCACTCAGTAGGAAGGTGGCCGAGTTGGTTCATTTTC
TGCTCCTCAAGTATCGAGCCAGGGAGCCGGTCACAAAGGCAGAAATGCTGGGGAGTGTCGTCGGAAATTG
GCAGTATTTCTTTCCTGTGATCTTCAGCAAAGCTTCCAGTTCCTTGCAGCTGGTCTTTGGCATCGAGCTG
ATGGAAGTGGACCCCATCGGCCACTTGTACATCTTTGCCACCTGCCTGGGCCTCTCCTACGATGGCCTGC
TGGGTGACAATCAGATCATGCCCAAGGCAGGCCTCCTGATAATCGTCCTGGCCATAATCGCAAGAGAGGG
CGACTGTGCCCCTGAGGAGAAAATCTGGGAGGAGCTGAGTGTGTTAGAGGTGTTTGAGGGGAGGGAAGAC
AGTATCTTGGGGGATCCCAAGAAGCTGCTCACCCAACATTTCGTGCAGGAAAACTACCTGGAGTACCGGC
AGGTCCCCGGCAGTGATCCTGCATGTTATGAATTCCTGTGGGGTCCAAGGGCCCTCGTTGAAACCAGCTA
TGTGAAAGTCCTGCACCATATGGTAAAGATCAGTGGAGGACCTCACATTTCCTACCCACCCCTGCATGAG
TGGGTTTTGAGAGAGGGGGAAGAGTGAGTCTGAGCACGAGTTGCAGCCAGGGCCAGTGGGAGGGGGTCTG
GGCCAGTGCACCTTCCGGGGCCGCATCCCTTAGTTTCCACTGCCTCCTGTGACGTGAGGCCCATTCTTCA
CTCTTTGAAGCGAGCAGTCAGCATTCTTAGTAGTGGGTTTCTGTTCTGTTGGATGACTTTGAGATTATTC
TTTGTTTCCTGTTGGAGTTGTTCAAATGTTCCTTTTAACGGATGGTTGAATGAGCGTCAGCATCCAGGTT
TATGAATGACAGTAGTCACACATAGTGCTGTTTATATAGTTTAGGAGTAAGAGTCTTGTTTTTTACTCAA
ATTGGGAAATCCATTCCATTTTGTGAATTGTGACATAATAATAGCAGTGGTAAAAGTATTTGCTTAAAAT
TGTGAGCGAATTAGCAATAACATACATGAGATAACTCAAGAAATCAAAAGATAGTTGATTCTTGCCTTGT
ACCTCAATCTATTCTGTAAAATTAAACAAATATGCAAACCAGGATTTCCTTGACTTCTTTGAGAATGCAA
GCGAAATTAAATCTGAATAAATAATTCTTCCTCTTC

Protein Information

Protein Name

transcript variant X2

NCBI Protein GI

4885467

Protein Accession

NP_005353

Protein pI

4.25

Protein Weight

32564.28

Protein Length

314

Protein Sequence

>NP_005353.1 melanoma-associated antigen 3 [Homo sapiens]
MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASS
LPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAELVHFLLLKYRAREPVTKAEMLGSVV
GNWQYFFPVIFSKASSSLQLVFGIELMEVDPIGHLYIFATCLGLSYDGLLGDNQIMPKAGLLIIVLAIIA
REGDCAPEEKIWEELSVLEVFEGREDSILGDPKKLLTQHFVQENYLEYRQVPGSDPACYEFLWGPRALVE
TSYVKVLHHMVKISGGPHISYPPLHEWVLREGEE

Epitope Information

IEDB Linear Epitope