LmSTI1
General Information
Protegen ID
736
Sequence Strain (Species/Organism)
Leishmania major
VO ID
VO_0011274
Taxonomy ID
5664
Other Database IDs
CDD:290150
Molecule Role
Protective antigen
Molecule Role Annotation
Researchers evaluated the potency of different DNA vaccine candidates in BALB/c mice using high dose footpad parasite challenge with L. major . Mice inoculated pCMV3ISS-LmSTI1 developed significantly smaller lesions in their footpads after challenge with the parasite. The vaccine was able to induce a partially protective effect (Ahmed et al. , 2009 ).
Related Vaccines(s)
L. major DNA Vaccine encoding LACKp24, TSA, LmSTI1 and CPa
,
L. major DNA vaccine encoding TSA, LMSTI1, KMP11, and LACK
,
LEISH-F1+MPL-SE
References
Ahmed et al. , 2009: Ahmed SB, Touihri L, Chtourou Y, Dellagi K, Bahloul C. DNA based vaccination with a cocktail of plasmids encoding immunodominant Leishmania (Leishmania ) major antigens confers full protection in BALB/c mice. Vaccine . 2009; 27(1); 99-9106. [PubMed: 18951941 ].
Gene Information
Gene Name
LmSTI1
NCBI Gene ID
5649395
NCBI Nucleotide GI
1698879
DNA Sequence
>gi|1698879|gb|U73845.1|LMU73845 Leishmania major protein antigen LmSTI1 mRNA, partial cds
TGGACGCAACTGAGCTGAAGAACAAGGGGAACGAAGAGTTCTCCGCCGGCCGCTATGTGGAGGCGGTGAA
CTACTTCTCAAAGGCGATCCAGTTGGATGAGCAGAACAGTGTCCTCTACAGCAACCGCTCCGCCTGTTTT
GCAGCCATGCAGAAATACAAGGACGCGCTGGACGACGCCGACAAGTGCATCTCGATCAAGCCGAATTGGG
CCAAGGGCTACGTGCGCCGAGGAGCAGCTCTCCATGGCATGCGCCGCTACGACGATGCCATTGCCGCGTA
TGAAAAGGGGCTCAAGGTGGACCCTTCCAACAGCGGCTGCGCGCAGGGCGTGAAGGACGTGCAGGTAGCC
AAGGCCCGCGAAGCACGTGACCCCATCGCTCGCGTCTTCACCCCGGAGGCGTTCCGCAAGATCCAAGAGA
ATCCCAAGCTGTCTCTACTTATGCTGCAGCCGGACTACGTGAAGATGGTAGACACCGTCATCCGCGACCC
TTCGCAGGGCCGGCTGTACATGGAAGACCAGCGCTTTGCCCTGACGCTCATGTACCTGAGCGGAATGAAG
ATTCCCAACGATGGTGATGGCGAGGAGGAGGAACGTCCGTCTGCGAAGGCGGCAGAGACAGCGAAGCCAA
AAGAGGAGAAGCCTCTCACCGACAACGAGAAGGAGGCCCTGGCGCTCAAGGAGGAGGGCAACAAGCTGTA
CCTCTCGAAGAAGTTTGAGGAGGCGCTGACCAAGTACCAAGAGGCGCAGGTGAAAGACCCCAACAACACT
TTATACATTCTGAACGTGTCGGCCGTGTACTTCGAGCAGGGTGACTACGACAAGTGCATCGCCGAGTGCG
AGCACGGTATCGAGCACGGTCGCGAGAACCACTGCGACTACACAATCATTGCGAAGCTCATGACCCGGAA
CGCCTTGTGCCTCCAGAGGCAGAGGAAGTACGAGGCTGCTATCGACCTTTACAAGCGCGCCCTTGTCGAG
TGGCGTAACCCTGACACCCTCAAGAAGCTGACGGAGTGCGAGAAGGAGCACCAAAAGGCGGTGGAGGAAG
CCTACATCGATCCTGAGATCGCGAAGCAGAAGAAAGACGAAGGTAACCAGTACTTCAAGGAGGATAAGTT
CCCCGAGGCCGTGGCAGCGTACACGGAGGCCATCAAGCGCAACCCTGCCGAGCACACCTCCTACAGCAAT
CGCGCGGCCGCGTACATCAAGCTTGGAGCCTTCAACGACGCCCTCAAGGACGCGGAGAAGTGCATTGAGC
TGAAGCCCGACTTTGTTAAGGGCTACGCGCGCAAGGGTCATGCTTACTTTTGGACCAAGCAGTACAACCG
CGCGCTGCAGGCGTACGATGAGGGCCTCAAGGTGGACCCGAGCAATGCGGACTGCAAGGATGGGCGGTAT
CGCACAATCATGAAGATTCAGGAGATGGCATCTGGCCAATCCGCGGATGGCGACGAGGCGGCGCGCCGGG
CCATGGACGATCCTGAAATCGCGGCAATCATGCAAGATAGCTACATGCAACTAGTGTTGAAGGAGATGCA
GAACGATCCCACGCGCATTCAGGAGTACATGAAGGACTCCGGGATCTCATCGAAGATCAACAAGCTGATT
TCAGCTGGCATCATTCGTTTTGGTCAGTAGACTTCTACGCTGCCTCATCTTTTCCGTGTCTTTGCGTCGG
CGGGTATCGTAAAGCACAATAAAGCAGCGATTCACATGCACGAGTAAAGTGCTGCGCCTCTCAAACACGA
CGTCGAGGCTGTGGTGCAGATGCGCGTCCTGCATGAAGGTAGTGAAGAGGAAAGTAAGGGATGTTGTTTG
TGGGCCTTCGTGGCTGCGCACACACCTCTTATCTCCTTCGCTTGGTACCTTCTCCCTTTTTCGTCTTCAC
CCCCCTTTCTCTTCTCACGCTCTCCCTGGCGCGGTGGTGCAACGATTTCGTTTTATTTACGTCTGTGTAG
CTCCTCTATTCAACGGTGCGATGACGCTAACGAAGCTGGCCTGTATTCGGCTAAGGCGAAGGCAAAAGAC
TAGGAGGGGGGGGGGAAGGAGACGGCGTGACCATCACTGCGAAGAAACAAGCCGAAGAAAAGGCCCCGAA
CGCCTGCATTTCCGCGCGCCCTCGCCCGCCTTCCTTCCTTCCTTCGCTCTCTCTCTCTCTCTCTCTCGCT
ATCTTCTCAACGGAGACATGAAAGGCGTTTGTTAGGAAAAGAGGGGGGGGGGAAGAGTGGGACGACGCGC
TGCGTCTTTTGGGCACTGGTCACGTGCGTCACCCTCTTTTTTTATCTCTATTGGCACTGTCTTGTTTCTT
TTCCCTTTCCTATCATACGCGTCTCGCAAACGACTCCGCGCTGAGCAGCCATGTGCTGCGGCGTGGAGGA
AGTACACAGACATCACGGATGCATATGTGCGCGTCCGTGTACGCGCTTGTATGGGGCTTCTAACAGCGCC
TGTGTGTGTTTGTGTGTGTGTGTGTGTGTGTGTCTGTGTATTTCGAGCGTCTGTATGCTATTCTATTAAG
CACCGAAGAAGAGACACACACGACAGCGAAGGAGATGGTGTCGGCTTTTCGGCTAATCACTCCCTTCCAT
AGCTTCTCTGAAGGAGGCTCTCTTCCAGAGGAATAGACTGCAGATGGGGTCCACGTTTATCTGAGGAGTC
AACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAG
Protein Information
Protein Name
protein antigen LmSTI1
NCBI Protein GI
1698880
Protein Accession
AAB37318.1
Protein pI
6.04
Protein Weight
60559.91
Protein Length
617
Protein Note
TPR repeat; pfam13414
Protein Sequence
>AAB37318.1 protein antigen LmSTI1, partial [Leishmania major]
DATELKNKGNEEFSAGRYVEAVNYFSKAIQLDEQNSVLYSNRSACFAAMQKYKDALDDADKCISIKPNWA
KGYVRRGAALHGMRRYDDAIAAYEKGLKVDPSNSGCAQGVKDVQVAKAREARDPIARVFTPEAFRKIQEN
PKLSLLMLQPDYVKMVDTVIRDPSQGRLYMEDQRFALTLMYLSGMKIPNDGDGEEEERPSAKAAETAKPK
EEKPLTDNEKEALALKEEGNKLYLSKKFEEALTKYQEAQVKDPNNTLYILNVSAVYFEQGDYDKCIAECE
HGIEHGRENHCDYTIIAKLMTRNALCLQRQRKYEAAIDLYKRALVEWRNPDTLKKLTECEKEHQKAVEEA
YIDPEIAKQKKDEGNQYFKEDKFPEAVAAYTEAIKRNPAEHTSYSNRAAAYIKLGAFNDALKDAEKCIEL
KPDFVKGYARKGHAYFWTKQYNRALQAYDEGLKVDPSNADCKDGRYRTIMKIQEMASGQSADGDEAARRA
MDDPEIAAIMQDSYMQLVLKEMQNDPTRIQEYMKDSGISSKINKLISAGIIRFGQ
Epitope Information
IEDB Linear Epitope
-- Assay Type --
T Cell Epitope
B Cell Epitope
IEDB ID
Epitope
Starting position
Ending position
DATELKNKGNEEFSAGRYVEAVNYFSKAIQLDEQNSVLYSNRSACFAAMQKYKDALDDADKCISIKPNWAKGYVRRGAALHGMRRYDDAIAAYEKGLKVDPSNSGCAQGVKDVQVAKAREARDPIARVFTPEAFRKIQENPKLSLLMLQPDYVKMVDTVIRDPSQGRLYMEDQRFALTLMYLSGMKIPNDGDGEEEERPSAKAAETAKPKEEKPLTDNEKEALALKEEGNKLYLSKKFEEALTKYQEAQVKDPNNTLYILNVSAVYFEQGDYDKCIAECEHGIEHGRENHCDYTIIAKLMTRNALCLQRQRKYEAAIDLYKRALVEWRNPDTLKKLTECEKEHQKAVEEAYIDPEIAKQKKDEGNQYFKEDKFPEAVAAYTEAIKRNPAEHTSYSNRAAAYIKLGAFNDALKDAEKCIELKPDFVKGYARKGHAYFWTKQYNRALQAYDEGLKVDPSNADCKDGRYRTIMKIQEMASGQSADGDEAARRAMDDPEIAAIMQDSYMQLVLKEMQNDPTRIQEYMKDSGISSKINKLISAGIIRFGQ
