VIOLIN: Vaccine Investigation and Online Information Network

Cpa

General Information

Protegen ID

737

Sequence Strain (Species/Organism)

Leishmania major

VO ID

VO_0011275

Taxonomy ID

5664

Other Database IDs

CDD:185513
CDD:285458
CDD:278538
CDD:239068

Molecule Role

Protective antigen

Molecule Role Annotation

Researchers evaluated the potency of different DNA vaccine candidates in BALB/c mice using high dose footpad parasite challenge with L. major. Mice inoculated pCMV3ISS-CPa developed significantly smaller lesions in their footpads after challenge with the parasite. The vaccine was able to induce a partially protective effect (Ahmed et al., 2009).

Related Vaccines(s)

L. major DNA Vaccine encoding LACKp24, TSA, LmSTI1 and CPa

References

Ahmed et al., 2009: Ahmed SB, Touihri L, Chtourou Y, Dellagi K, Bahloul C. DNA based vaccination with a cocktail of plasmids encoding immunodominant Leishmania (Leishmania) major antigens confers full protection in BALB/c mice. Vaccine. 2009; 27(1); 99-9106. [PubMed: 18951941].

Gene Information

Gene Name

Cpa

NCBI Gene ID

5651252

NCBI Nucleotide GI

71084305

DNA Sequence

>gi|71084305|gb|DQ071680.1| Leishmania major cysteine protease (Cpa) gene, partial cds
AAAACACCTCTCGGTGTCGACAACTTCATTGCCTCAGCACATTACGGACGCTTTAAGGAGCGCCACGGCA
AGTCCTTCGGCGAGGACGCCGATGAGGGTCACCGCTTCAATGCCTTCAAGCAGAACATGCAGACCGCCTA
CTTCCTCAACACGCACAACCCGCACGCGCACTACGACGTGTCCGGCAAGTTTGCAGACCTCACCCCCCAG
GAGTTCGCCAAGCTGTACCTGAACCCCGACTACTACGCGCGCCGCGGTAAGGATTACAAGGAGCACGTGC
ACGTCGACGACAGCGTCCTCAGTGGTGCGATGTCGGTGGACTGGCGTGAGAAGGTTGCCGTGACGCCGGT
GAAGAACCAGGGAATGTGCGGCTCGTGCTGGGCTTTCTCCGCCATTGGCAACATTGAAAGTCAGTGGGCT
TTGAAAAACCACTCGCTGGTTTCGCTGTCGGAGCAGATGCTCGTGTCATGCGACGACATCGATGATGGGT
GCAACGGTGGGCTGATGGACCAGGCGATGGAATGGATCATCCAACATCACAACGGCACTGTGCCCACGGA
GGAAAGCTACCCCTACGCCTCTGCCGGCGGCACGAGCCCGCCGTGCCATGACAAAGGCGAGTTTGGCGCC
AGAATCAGTGGTTACATGTCCCTGCCGCATGACGAGAAGGCGATCGCGGCTTATGTGGAGAAGAAAGGCC
CCGTCGCTGTCGCCGTCGACGCGACAACCTGGCAGCTGTACTTTGGCGGTGTGGTCACGCTCTGCTTCGG
GTGGTCGCTCAACCACGGTGTGCTCGTTGTCGGCTTCAACAAAAGAGCGAAACCGCCGTACTGGATCGTG
AAGAACTCGTGGGGCACCTCGTGGGGTGAGAAGGGGTACATCCGCCTTGCCATGGGCAGCAACCAGTGCT
TGCTGAAGAATTACCCTGTGACGGCCACGGTTGACGACTCAAACACCTCTCCCGTGCCGACGACACCGGC
CTAG

Protein Information

Protein Name

cysteine protease

NCBI Protein GI

71084306

Protein Accession

AAZ23598.1

Protein pI

6.62

Protein Weight

33970.17

Protein Length

391

Protein Note

cathepsin L protease; Provisional

Protein Sequence

>AAZ23598.1 cysteine protease, partial [Leishmania major]
KTPLGVDNFIASAHYGRFKERHGKSFGEDADEGHRFNAFKQNMQTAYFLNTHNPHAHYDVSGKFADLTPQ
EFAKLYLNPDYYARRGKDYKEHVHVDDSVLSGAMSVDWREKVAVTPVKNQGMCGSCWAFSAIGNIESQWA
LKNHSLVSLSEQMLVSCDDIDDGCNGGLMDQAMEWIIQHHNGTVPTEESYPYASAGGTSPPCHDKGEFGA
RISGYMSLPHDEKAIAAYVEKKGPVAVAVDATTWQLYFGGVVTLCFGWSLNHGVLVVGFNKRAKPPYWIV
KNSWGTSWGEKGYIRLAMGSNQCLLKNYPVTATVDDSNTSPVPTTPA

Epitope Information

IEDB Linear Epitope