VIOLIN: Vaccine Investigation and Online Information Network

M2

General Information

Protegen ID

754

Sequence Strain (Species/Organism)

Leishmania amazonensis

VO ID

VO_0011291

Taxonomy ID

5659

Other Database IDs

CDD:240315
CDD:153108

Molecule Role

Protective antigen

Molecule Role Annotation

Immunization of CBA mice with the M-2 glycoprotein of L. amazonensis and C. parvum adjuvant resulted in complete protection against a challenge infection of 10^4 and 10^6 late log-phase promastigotes of L. amazonensis. In the BALB/c strain, complete protection was observed in some of the immunized animals (28 to 50%); in the rest of the mice the onset of infection was significantly delayed. Protective immunity for C57BL/6 mice was observed only at the low infecting dose (10(4) L. amazonensis organisms) (Champsi and McMahon-Pratt, 1988).

Related Vaccines(s)

L. amazonensis M2 protein vaccine

References

Champsi and McMahon-Pratt, 1988: Champsi J, McMahon-Pratt D. Membrane glycoprotein M-2 protects against Leishmania amazonensis infection. Infection and immunity. 1988; 56(12); 3272-3279. [PubMed: 3182080].

Gene Information

Gene Name

NCBI Nucleotide GI

2828562

DNA Sequence

>gi|2828562|gb|AF005247.1| Leishmania mexicana amazonensis extrachromosomal element ribonucleotide reductase M2 subunit gene, complete cds
ATGAAACCGGTTGCGGCTGGCGCCGAGGTGCTGCCGGCGGACAAGGTTGCCCAGGGGACGAACGCCGAGG
AGGAGCCGCTGCAGCAGGAGAACCCGTTCCGCTACGTCCTCTTCCCAATCCAGTACCATGACATCTGGCG
GAAGTACAAGGAGCAGGAGAGCTGCATCTGGACGGTGGAGGAGATCGACCTGGGCAACGACATGAAGGAC
TGGGCAACGCTGAACGACGGTGAGCGGCACTTCATCAAGCATGTGCTTGCCTTCTTCGCCGGCAGCGACG
GCATAGTCATCGAGAATCTTGCGCAGCGCTTCATGAGCGACGTGAAGGTGCCAGAGGCGCGCGCGTTCTA
CGGGTTCCAGCTGATGATGGAGAACATCCACTCGGAGACGTACTCTGTGCTGCTGGACACGTACATCACG
GACAGCGAGGAGAAGCTGCGGCTGCTGCACGCGATACAGACGATCCCGTGCATCCAGAAGAAGGCGGAGT
GGCCCGTGCGGTGGATCGGGAGCAGCGCGAGCTTTCAGCAGCGGCTGATCGGCTTTGCCGCGGTGGAGGG
CATTTTCTTTTCCGGGTCGTTCTGCGCGCTGTTCTGGCTGAAGAAGCGCGGTCTGATGCCAGGGCTGACG
TTCAGCAACGAGCTCATCTCGCGCGACGAGGGTCTACACACGGACTTCGCATGCCTGCTGTACAACTCGC
ACATCAAGCACAAGCTGCCGCGCGAGCGCGTGCTGGAGATCATCGTGGATGCGGTGAACATCGAGCGTGA
GTTCATCTGCGACGCGCTGCCGGTGCGGCTGATTGGCATGAACGCGGAGCTGATGGCGCAGTACATCGAG
TTCGTCGCGGACCGGCTGCTGGTGTCGCTCGGCGAGGAGAAGCACTACCGCTCGACGCAGCCGTTCGACT
TCATGGAGATGATCTCGCTGCAGGGCAAAACGAACTTCTTCGAGAAGAGGGTGGGGGAGTACCAGAAGGC
CGGCGTGATGAGCACGGAGGGCACGTCGAAAAAGTTCTCCCTCTCGGAGGACTTCTAA

Protein Information

Protein Name

ribonucleotide reductase M2 subunit

NCBI Protein GI

2828563

Protein Accession

AAC08302.1

Protein pI

5.07

Protein Weight

38155.95

Protein Length

424

Protein Note

hydroxyurea-resistant strain derived from LV-78;
cloned from extrachromosomal amplified circular DNA

Protein Sequence

>AAC08302.1 ribonucleotide reductase M2 subunit [Leishmania amazonensis]
MKPVAAGAEVLPADKVAQGTNAEEEPLQQENPFRYVLFPIQYHDIWRKYKEQESCIWTVEEIDLGNDMKD
WATLNDGERHFIKHVLAFFAGSDGIVIENLAQRFMSDVKVPEARAFYGFQLMMENIHSETYSVLLDTYIT
DSEEKLRLLHAIQTIPCIQKKAEWPVRWIGSSASFQQRLIGFAAVEGIFFSGSFCALFWLKKRGLMPGLT
FSNELISRDEGLHTDFACLLYNSHIKHKLPRERVLEIIVDAVNIEREFICDALPVRLIGMNAELMAQYIE
FVADRLLVSLGEEKHYRSTQPFDFMEMISLQGKTNFFEKRVGEYQKAGVMSTEGTSKKFSLSEDF

Epitope Information

IEDB Linear Epitope