VIOLIN: Vaccine Investigation and Online Information Network

Cruzipain

General Information

Protegen ID

756

Sequence Strain (Species/Organism)

Trypanosoma cruzi

VO ID

VO_0011293

Taxonomy ID

5693

Other Database IDs

CDD:185513
CDD:214853
CDD:278538
CDD:239068
CDD:288946

Molecule Role

Protective antigen

Molecule Role Annotation

To study the protective effects in vivo of cruzipain-specific Th1 responses against systemic T. cruzi challenges, mice were immunized with recombinant cruzipain plus interleukin 12 (IL-12) and a neutralizing anti-IL-4 MAb. These immunized mice developed potent cruzipain-specific memory Th1 cell responses and were significantly protected against normally lethal systemic T. cruzi challenges. To study whether cruzipain could induce mucosal immune responses relevant for vaccine development, recombinant attenuated Salmonella enterica serovar Typhimurium vaccines expressing cruzipain were prepared. BALB/c mice immunized with salmonella expressing cruzipain were significantly protected against T. cruzi mucosal infection (Schnapp et al., 2002).

Related Vaccines(s)

T. cruzi vaccine using attenuated Salmonella expressing T. cruzi Cruzipain

References

Schnapp et al., 2002: Schnapp AR, Eickhoff CS, Sizemore D, Curtiss R 3rd, Hoft DF. Cruzipain induces both mucosal and systemic protection against Trypanosoma cruzi in mice. Infection and immunity. 2002; 70(9); 5065-5074. [PubMed: 12183554].

Gene Information

Gene Name

Cruzipain

NCBI Gene ID

3550848

Genbank Accession

AAHK01000135

Chromosome No

Unknown

Gene Starting Position

102406

Gene Ending Position

103809

DNA Sequence

>NW_001849391.1:102406-103809 Trypanosoma cruzi strain CL Brener 1047053516253 genomic scaffold, whole genome shotgun sequence
AATGTCTGGCTGGGCGCGTGCGCTGTTGCTCGCGGCCGTCCTGGTCGTCATGGCGTGCCTTGTCCCCGCG
GCGACGGCGAGCCTGCATGCGGAGGAGACGCTGACGTCGCAATTCGCAGAATTCAAGCAGAAGCATGGCA
GGGTGTACGAGAGCGCCGCGGAGGAGGCGTTCCGCCTGAGCGTGTTCAGGGAGAACCTGTTTCTTGCGAG
GCTGCACGCCGCGGCAAACCCACACGCGACCTTCGGCGTCACGCCCTTCTCGGACCTCACGCGCGAGGAA
TTCCGGTCCCGCTACCACAACGGCGCGGCGCACTTTGCGGCGGCGCAGGAGCGCGCGAGAGTGCCGGTGA
AGGTGGAGGTAGTTGGCGCGCCCGCGGCAGTGGATTGGCGTGCGAGAGGCGCCGTGACAGCCGTCAAGGA
CCAGGGCCAATGCGGTTCGTGCTGGGCCTTCTCCGCCATTGGTAACGTTGAGTGCCAGTGGTTTCTTGCC
GGCCACCCGCTGACGAACCTGTCGGAGCAGATGCTCGTGTCGTGCGACAAAACGGACTTTGGCTGCAGTG
GTGGCCTGATGAACAACGCCTTTGAGTGGATTGTGCAGGAGAATAACGGCGCCGTGTACACGGAGGACAG
CTACCCTTATGCGTCGGGCGAGGGGATATCGCCGCCGTGCACGACGTCAGGCCACACGGTGGGTGCCACG
ATTACCGGTCACGTTGAATTACCTCAGGACGAGGCCCAAATAGCCGCATGGCTTGCAGTCAATGGCCCGG
TTGCCGTTGCCGTCGACGCCAGCAGCTGGATGACCTACACGGGCGGCGTTATGACGAGCTGCGTCTCCGA
GCAGCTGGATCACGGCGTTCTTCTCGTCGGCTACAATGACAGCGCCGCAGTGCCGTACTGGATCATCAAG
AACTCGTGGACCACGCAGTGGGGCGAGGAAGGCTACATCCGCATTGCAAAGGGCTCGAACCAGTGCCTTG
TCAAGGAGGAGGCGAGCTCCGCGGTGGTCGGTGGTCCCGGACCCACTCCCGAGCCAACCACCACGACAAC
CACAAGTGCCCCAGGACCGTCCCCATCGTACTTTGTGCAGATGTCCTGCACTGACGCTGCGTGCATTGTC
GGGTGCGAGAACGTGACGTTACCGACCGGTCAGTGTCTCCTGACCACCAGCGGCGTCTCTGCCATTGTCA
CGTGCGGTGCTGAGACTCTCACAGAAGAAGTCTTCCTTACGAGTACGCACTGCAGCGGCCCATCGGTGAG
GTCCTCTGTTCCTCTCAACAAATGCAACCGGCTTTTAAGAGGCTCCGTTGAGTTCTTCTGCGGCTCCAGC
TCCAGTGGCCGACTGGCCGACGTGGACAGGCAGCGTCGCCATCAGCCATACCACAGCCGTCATCGCCGCC
TCTG

Protein Information

Protein Name

cruzipain

NCBI Protein GI

71663165

Protein Accession

XP_818579

Protein pI

6.01

Protein Weight

45629.84

Protein Length

467

Protein Note

internal copy from a tandem array

Protein Sequence

>XP_818579.1 cruzipain precursor, putative [Trypanosoma cruzi]
MSGWARALLLAAVLVVMACLVPAATASLHAEETLTSQFAEFKQKHGRVYESAAEEAFRLSVFRENLFLAR
LHAAANPHATFGVTPFSDLTREEFRSRYHNGAAHFAAAQERARVPVKVEVVGAPAAVDWRARGAVTAVKD
QGQCGSCWAFSAIGNVECQWFLAGHPLTNLSEQMLVSCDKTDFGCSGGLMNNAFEWIVQENNGAVYTEDS
YPYASGEGISPPCTTSGHTVGATITGHVELPQDEAQIAAWLAVNGPVAVAVDASSWMTYTGGVMTSCVSE
QLDHGVLLVGYNDSAAVPYWIIKNSWTTQWGEEGYIRIAKGSNQCLVKEEASSAVVGGPGPTPEPTTTTT
TSAPGPSPSYFVQMSCTDAACIVGCENVTLPTGQCLLTTSGVSAIVTCGAETLTEEVFLTSTHCSGPSVR
SSVPLNKCNRLLRGSVEFFCGSSSSGRLADVDRQRRHQPYHSRHRRL

Epitope Information

IEDB Linear Epitope