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CP cysteine proteinase

General Information
Protegen ID 758
Sequence Strain (Species/Organism) Leishmania amazonensis
VO ID VO_0011295
Taxonomy ID 5659
Other Database IDs CDD:185513
CDD:214853
CDD:278538
CDD:239068
Molecule Role Protective antigen
Molecule Role Annotation A 500 bp fragment encoding an isoform of cysteine proteinase (CP) from Leishmania (Leishmania) amazonensis was subcloned and expressed in the pHis vector, resulting in a recombinant protein of 24 kDa, rLacys24. Immunization of BALB/c mice with rLacys24 plus CFA adjuvant resulted in a low but significant decrease of foot lesions after challenge with L. (L.) amazonensis compared to those exhibited by control mice (Fedeli et al., 2010).
Related Vaccines(s) L. amazonensis CP Protein Vaccine
References
Fedeli et al., 2010: Fedeli CE, Ferreira JH, Mussalem JS, Longo-Maugéri IM, Gentil LG, dos Santos MR, Katz S, Barbiéri CL. Partial protective responses induced by a recombinant cysteine proteinase from Leishmania (Leishmania) amazonensis in a murine model of cutaneous leishmaniasis. Experimental parasitology. 2010; 124(2); 153-158. [PubMed: 19735658].
Gene Information
Gene Name CP cysteine proteinase
NCBI Nucleotide GI 30142041
DNA Sequence 
>gi|30142041|gb|AY141759.1| Leishmania mexicana amazonensis clone 2A1 cysteine proteinase mRNA, complete cds
CCGTCACGCCCTTCTCGTGCGCACGTTGCAGAACCAGGATCGGCAAAGATGGCGCGCCGCAACCCCCTTT
TGTTTGCGATAGTGGTGACGATCCTGTTCGTGGTGTGCTACGGTTCCGCTCTCATCGCCCAGACACCCCC
CGCCGTCGACAACTTCGTTGCCTCAGCGCATTACGGAAGTTTTAAGAAGCGCCACAGTAAGGCCTTCGGC
GGGGACGCCGAGGAGGGTCACCGCTTCAATGCCTTCAAGCAGAACATGCAGACAGCCTACTTCCTCAACA
CGCAGAACCCCCACGCGCACTACGACGTGTCCGGCAAGTTCGCGGACCTCACCCCGCAGGAGTTCGCCAA
GCTGTACCTGAATCCCGACTACTACACGAGCCACCTCAAGGATCACAAGGAGGACGTGCACGTCGACGAC
AGCGCCCCCAGTGGTGTGATGTCGGTGGACTGGCGTGATAAGGGTGCCGTGACGCCGGTGAAGAACCAGG
GATTATGCGGCTCGTGCTGGGCCTTCTCCGCCATTGGCAACATCGAAGGTCAGTGGGCTGCAAGCGGCCA
CTCGTTGGTTTCACTGTCAGAACAGATGCTCGTGTCGTGCGACAACGTCGATGAAGGGTGCAACGGCGGG
CTGATGGACCAGGCAATGAACTGGATCATGCAGAGTCACAACGGCAGTGTGTTCACGGAGGCCAGCTATC
CCTACACCTCTGGCGGCGGCACCAGACCGCCGTGCCATGACGAAGGTGAAGTTGGCGCTAAAATCACCGG
TTTCCTCTCCCTGCCGCACGACGAGGAGCGGATCGCGGACTGGGTGGAGAAGAGAGGCCCCGTCGCCGTC
GCCGTCGACGCGACAACCTGGCAGCTGTACTTTGGCGGTGTGGTCTCCCTCTGCCTCGCGTGGTCGCTCA
ACCACGGTGTGCTCATTGTCGGCTTCAACAAAAACGCGAAACCGCCGTACTGGATCGTGAAGAACTCGTG
GGGCTCCTCGTGGGGTGAGAAGGGGTACATCCGCCTTGCCATGGGCAGCAACCAGTGCATGCTCAAGAAT
TACCCCGTGTCGGCCACGGTTGAAAGCCCCCACACCCCACACGTGCCGACGACAACGGCCTAGCTGGAGC
CCAGGCCCACGGCGCCTGGGCGAATGAGCTGCTTTTTTTTTCGAGTGGGTGCTCGAGGCTGTGCACAAGC
ACGACCTATCCGACCTGTGTGTGCCTGAAGCGCCAGAATGGCGGCTCTGCCCAGGTGACCTGTGGCAAAC
CCGAGACGGCGGAGCTTCTCTACAACTGGCCGGGTTGCTCCGAGGCTGCCACCGAGGCCTGCATGCCGCT
GAACAATATGTATGCGCAGCTACGCCGGCTACCTCCAGAACGTCTGCACTGCGTTCGTCGCTTGCACTGA
GAACCAACGACGCCGTCTCTGACTTGACCAAGCCAACCATCTTGGGCCAACCACACGTCCCGTACGCGAG
GCACGCGGGGATCCAGGACTGGCAGGATCCACTGCGCACTATACGCCTCAGCTGCACCGATGCGGATCCC
TCCCTCAAAAAAAAAAAAAAAAAAAAAA
Protein Information
Protein Name cysteine proteinase
NCBI Protein GI 30142042
Protein Accession AAN34826.1
Protein pI 6.56
Protein Weight 36075.3
Protein Length 418
Protein Note forma: amastigote
Protein Sequence 
>AAN34826.1 cysteine proteinase [Leishmania amazonensis]
MARRNPLLFAIVVTILFVVCYGSALIAQTPPAVDNFVASAHYGSFKKRHSKAFGGDAEEGHRFNAFKQNM
QTAYFLNTQNPHAHYDVSGKFADLTPQEFAKLYLNPDYYTSHLKDHKEDVHVDDSAPSGVMSVDWRDKGA
VTPVKNQGLCGSCWAFSAIGNIEGQWAASGHSLVSLSEQMLVSCDNVDEGCNGGLMDQAMNWIMQSHNGS
VFTEASYPYTSGGGTRPPCHDEGEVGAKITGFLSLPHDEERIADWVEKRGPVAVAVDATTWQLYFGGVVS
LCLAWSLNHGVLIVGFNKNAKPPYWIVKNSWGSSWGEKGYIRLAMGSNQCMLKNYPVSATVESPHTPHVP
TTTA
Epitope Information
IEDB Linear Epitope