VIOLIN: Vaccine Investigation and Online Information Network

E1

General Information

Protegen ID

774

Sequence Strain (Species/Organism)

Western equine encephalomyelitis virus

VO ID

VO_0011306

Taxonomy ID

11039

Other Database IDs

CDD:279870

Molecule Role

Protective antigen

Molecule Role Annotation

DNA vaccines encoding different portions of the structural proteins of western equine encephalitis virus were tested for the efficacy of their protection in a 100% lethal mouse model of the virus. The 6K-E1 structural protein encoded by the DNA vaccine conferred complete protection against challenge with the homologous strain and limited protection against challenge with a heterologous strain (Gauci et al., 2010).

Note: The E1 glycoprotein is part of the 6K-E1 structural protein

Additional Molecule Role

Virmugen

Additional Molecule Role Annotation

A PE2 mutant combined with a deletion in E1 (WE2130) induced low-level viremia in chickens and protected against challenge with wild type WEE after 2 weeks. This study also found that it is unlikely that mosquito transmission of this strain would occur (Turell et al., 2003).

Related Vaccines(s)

WEEV DNA Vaccine encoding 6K-E1 Protein , Western equine encephalomyelitis virus PE2/E1 mutant vaccine

References

Gauci et al., 2010: Gauci PJ, Wu JQ, Rayner GA, Barabé ND, Nagata LP, Proll DF. Identification of Western equine encephalitis virus structural proteins that confer protection after DNA vaccination. Clinical and vaccine immunology : CVI. 2010; 17(1); 176-179. [PubMed: 19923571].

Turell et al., 2003: Turell MJ, O'Guinn ML, Parker MD. Limited potential for mosquito transmission of genetically engineered, live-attenuated western equine encephalitis virus vaccine candidates. The American journal of tropical medicine and hygiene. 2003; 68(2); 218-221. [PubMed: 12641414].

Gene Information

Gene Name

NCBI Nucleotide GI

17148716

DNA Sequence

>gi|17148716|gb|AF398393.1| Western equine encephalomyelitis virus glycoprotein (E1) mRNA, partial cds
AGACGCACAGTGCTTTTGTGACAGCGAGAACACGCAACTAAGCGAAGCATATGTCGAGTTTGCTCCAGAT
TGCACTGTAGATCACGCAGTCGCTTTAAAAGTTCACACAGCTGCTTTGAAAGTCAGCCTGCGCATAGTAT
ATGGCAATACCACCGCGCGCCTGGATACGTTCGTTAATGGCGTCACACCAGGTTCCACGCGAGACCTGAA
GGTCATCGCAGGGCCGATATCAGCCGCTTTTTCGCCCTTTGACCATAAAGTCGTTATTAGAAAAGGGCTT
GTCTACAACTATGACTTTCCAGAGTACGGAGCCATGAGACCAGGTGTGTTCGGCGATATTCAAGCATCTT
CGCTAGACGCCAAGGACATAGTAGCCCGCACTGACATACGGCTGCTGAAGCCTTCTGTCAAGAACATCCA
TGTTCCTTACACCCAAGCAGTGTCAGGGTATGAAATGTGGAAGAACAATTCAGGACGGCCACTGCAAGAA
ACGGCACCGTTTGGCTGTAAAATCGAAGTGGAGCCTTTGCGAGCGTCTGATTGCGCTTATGGGCACATCC
CTATCTCAATCGATATTCCTGACGCAGCTTTCGTGAGATCATCAGAGTCACCTACAATTCTAGAAGTCAG
CTGCTCGGTAGCAGACTGCATCTATTCGGCAGACTTTGGAGGCTCACTGACACTACAGTACAAAGCTGAC
AGGGAGGGACACTGTCCAGTTCACTCTCACTCCACTACAGCTGTCTTGAAGGAAGCGACCACACACGTGA
CTGCCATAGGCAGCATAACGCTACATTTCAGTACATCAAGCCCACAAGCAAATTTTATAATTTCGCTGTG
TGGCAAGAAGACTACTTGCAATGCTGAATGCAAACCACCAGAGGACCACATAATTGGTGAACCACACAAG
GTTGACCAGGAATTCCAGGCGGCAGTTTCTAAAACATCCTGGAACTGGCTCGTCGCGCTGTTTGGGGGAG
CATCATCCCTCATTGTTGTAGGACT

Protein Information

Protein Name

glycoprotein

NCBI Protein GI

17148717

Protein Accession

AAL35792.1

Protein pI

6.22

Protein Weight

33702.07

Protein Length

411

Protein Note

Alphavirus E1 glycoprotein; pfam01589

Protein Sequence

>AAL35792.1 glycoprotein, partial [Western equine encephalitis virus]
DAQCFCDSENTQLSEAYVEFAPDCTVDHAVALKVHTAALKVSLRIVYGNTTARLDTFVNGVTPGSTRDLK
VIAGPISAAFSPFDHKVVIRKGLVYNYDFPEYGAMRPGVFGDIQASSLDAKDIVARTDIRLLKPSVKNIH
VPYTQAVSGYEMWKNNSGRPLQETAPFGCKIEVEPLRASDCAYGHIPISIDIPDAAFVRSSESPTILEVS
CSVADCIYSADFGGSLTLQYKADREGHCPVHSHSTTAVLKEATTHVTAIGSITLHFSTSSPQANFIISLC
GKKTTCNAECKPPEDHIIGEPHKVDQEFQAAVSKTSWNWLVALFGGASSLIVVGL

Vaxign Prediction

Localization(Probability)

(Prob.=0)

Adhesin Probability

0.506

Trans-membrane Helices

Detailed Vaxign Results

Vaxign Results

Epitope Information

IEDB Linear Epitope