VIOLIN: Vaccine Investigation and Online Information Network

RVFVsMgp1 M protein

General Information

Protegen ID

787

Sequence Strain (Species/Organism)

Rift Valley fever virus

VO ID

VO_0011318

Taxonomy ID

11588

Molecule Role

Protective antigen

Molecule Role Annotation

The glycoprotein (GP) and nucleocapsid (NC) genes of Rift Valley fever virus (RVFV) were expressed in different expression systems and were evaluated for their ability to protect mice from virulent challenge using a prime-boost regime. Mice vaccinated with a lumpy skin disease virus-vectored recombinant vaccine (rLSDV-RVFV) expressing the two RVFV glycoproteins (G1 and G2, expressed by the RVFV M Segment) developed neutralising antibodies and were fully protected when challenged, as were those vaccinated with a crude extract of truncated G2 glycoprotein (tG2) (Wallace et al., 2006).

Related Vaccines(s)

RVF MP-12

References

Wallace et al., 2006: Wallace DB, Ellis CE, Espach A, Smith SJ, Greyling RR, Viljoen GJ. Protective immune responses induced by different recombinant vaccine regimes to Rift Valley fever. Vaccine. 2006; 24(49-50); 7181-7189. [PubMed: 16870311].

Gene Information

Gene Name

RVFVsMgp1 M protein

NCBI Gene ID

956559

Genbank Accession

M25276

Locus Tag

RVFVsMgp1

Gene Starting Position

Gene Ending Position

3613

DNA Sequence

>NC_002044.2:20-3613 Rift Valley fever virus M segment, complete sequence
AATGTATGTTTTATTAACAATTCTAACCTCGGTTCTGGTGTGTGAAGCGATTATTAGAGTGTCTCTAAGC
TCCACAAGAGAAGAGACCTGCTTTGGTGACTCCACTAACCCAGAGATGATTGAAGGAGCTTGGGATTCAC
TCAGAGAGGAGGAGATGCCGGAGGAGCTCTCCTGTTCTATATCAGGCATAAGAGAGGTTAAGACCTCAAG
CCAGGAGTTATACAGGGCATTAAAAGCCATCATTGCTGCTGATGGCTTGAACAACATCACCTGCCATGGT
AAGGATCCTGAGGACAAGATTTCCCTCATAAAGGGTCCTCCTCACAAAAAGCGGGTGGGGATAGTTCGGT
GTGAGAGACGAAGAGATGCTAAGCAAATAGGGAGAAAAACCATGGCAGGGATTGCAATGACAGTCCTTCC
AGCCTTAGCAGTTTTTGCTTTGGCACCTGTTGTTTTTGCTGAAGACCCCCATCTCAGAAACAGACCAGGG
AAGGGGCACAACTACATTGACGGGATGACTCAGGAGGATGCCACATGCAAACCTGTGACATATGCTGGGG
CATGTAGCAGTTTTGATGTCTTGCTTGAAAAGGGAAAATTTCCCCTTTTCCAGTCGTATGCTCATCATAG
AACTCTACTAGAGGCAGTTCACGACACCATCATTGCAAAGGCTGATCCACCTAGCTGTGACCTTCTGAGT
GCTCATGGGAACCCCTGCATGAAAGAGAAACTCGTGATGAAGACACACTGTCCAAATGACTACCAGTCAG
CTCATCACCTCAACAATGACGGGAAAATGGCTTCAGTCAAGTGCCCTCCTAAGTATGAGCTCACTGAAGA
CTGCAACTTTTGTAGGCAGATGACAGGTGCTAGCCTGAAGAAGGGGTCTTATCCTCTCCAAGACTTGTTT
TGTCAGTCAAGTGAGGATGATGGATCAAAATTAAAAACAAAAATGAAAGGGGTCTGCGAAGTGGGGGTTC
AAGCACTCAAAAAGTGTGATGGCCAACTCAGCACTGCACATGAGGTTGTGCCCTTTGCAGTGTTTAAGAA
CTCAAAGAAGGTTTATCTTGATAAGCTTGACCTTAAGACTGAGGAGAATCTGCTACCAGACTCATTTGTC
TGTTTCGAGCATAAGGGACAGTACAAAGGAACAATGGACTCTGGTCAGACTAAGAGGGAGCTCAAAAGCT
TTGATATCTCTCAGTGCCCCAAGATTGGAGGACATGGTAGTAAGAAGTGCACTGGGGACGCAGCATTTTG
CTCTGCTTATGAGTGCACTGCTCAGTACGCCAATGCCTATTGTTCACATGCTAATGGGTCAGGGATTGTG
CAGATACAAGTATCAGGGGTCTGGAAGAAGCCTTTATGTGTAGGGTATGAGAGAGTGGTTGTGAAGAGAG
AACTCTCTGCCAAGCCCATCCAGAGAGTTGAGCCTTGCACAACTTGTATAACCAAATGTGAGCCTCATGG
ATTGGTTGTCCGATCAACAGGGTTCAAGATATCATCAGCAGTTGCTTGTGCTAGCGGAGTTTGCGTCACA
GGATCGCAGAGTCCTTCCACCGAGATTACACTCAAGTATCCAGGGATATCCCAGTCTTCTGGGGGGGACA
TAGGGGTTCACATGGCACACGATGATCAGTCAGTTAGCTCCAAAATAGTAGCTCACTGCCCTCCCCAGGA
CCCGTGCTTAGTGCATGACTGCATAGTGTGTGCTCATGGCCTGATAAATTACCAGTGTCACACTGCTCTC
AGTGCCTTTGTTGTTGTGTTTGTATTCAGTTCTATTGCAATAATTTGTTTAGCTATTCTTTATAGGGTGC
TTAAGTGCCTGAAGATTGCCCCAAGGAAAGTTCTGAATCCACTAATGTGGATCACAGCCTTCATCAGATG
GATATATAAGAAGATGGTTGCCAGAGTGGCAGACAACATTAATCAAGTGAACAGGGAAATAGGATGGATG
GAAGGAGGTCAGTTGGTTCTAGGGAACCCTGCCCCTATTCCTCGTCATGCCCCAATCCCACGTTATAGCA
CATACCTGATGTTATTATTGATTGTCTCATATGCATCAGCATGTTCAGAACTGATTCAGGCAAGCTCCAG
AATCACCACTTGCTCTACAGAGGGTGTTAACACCAAGTGTAGACTGTCTGGCACAGCATTGATCAGAGCA
GGGTCAGTTGGGGCAGAGGCTTGTTTGATGTTGAAGGGGGTCAAGGAAGATCAAACCAAGTTCTTAAAGT
TAAAAACTGTCTCAAGTGAGCTATCATGCAGGGAGGGCCAGAGTTATTGGACTGGGTCCTTTAGCCCTAA
ATGTTTGAGCTCAAGGAGATGCCACCTTGTCGGGGAATGCCATGTGAATAGGTGTCTGTCTTGGAGGGAC
AATGAAACTTCAGCAGAGTTTTCATTTGTTGGGGAAAGCACGACCATGCGAGAGAATAAGTGTTTTGAGC
AATGTGGAGGATGGGGGTGTGGGTGTTTCAATGTGAACCCATCTTGCTTATTTGTGCACACGTATCTGCA
GTCAGTTAGAAAAGAGGCCCTTAGAGTTTTTAACTGTATCGACTGGGTGCATAAACTCACTCTAGAGATC
ACAGACTTTGATGGCTCTGTTTCAACAATAGACTTGGGAGCATCATCTAGCCGTTTCACAAACTGGGGTT
CAGTTAGCCTCTCACTGGACGCAGAGGGCATTTCAGGCTCAAATAGCTTTTCTTTCATTGAGAGCCCAGG
CAAAGGGTATGCAATTGTTGATGAGCCATTCTCAGAAATTCCTCGGCAAGGGTTCTTGGGGGAGATCAGG
TGCAATTCAGAGTCCTCAGTCCTGAGTGCTCATGAATCATGCCTTAGGGCACCAAACCTTATCTCATACA
AGCCCATGATAGATCAATTGGAGTGCACAACAAATCTGATTGATCCCTTTGTTGTCTTTGAGAGGGGTTC
TCTGCCACAGACAAGGAATGACAAAACCTTTGCAGCTTCAAAAGGAAATAGAGGTGTTCAAGCTTTCTCT
AAGGGCTCTGTACAAGCTGATCTAACTCTGATGTTTGACAATTTTGAGGTGGACTTTGTGGGAGCAGCCG
TATCTTGTGATGCCGCCTTCTTAAATTTGACAGGTTGCTATTCTTGCAATGCAGGGGCCAGGGTCTGCCT
GTCTATCACATCCACAGGAACTGGATCTCTCTCTGCCCACAATAAGGATGGGTCTCTGCATATAGTCCTT
CCATCAGAGAATGGAACAAAAGACCAGTGTCAGATACTACACTTCACTGTGCCTGAAGTAGAGGAGGAGT
TTATGTACTCTTGTGATGGAGATGAGCGGCCTCTGTTGGTGAAGGGGACCCTGATAGCCATTGATCCATT
TGATGATAGGCGGGAAGCAGGGGGGGAATCAACAGTTGTGAATCCAAAATCTGGATCTTGGAATTTCTTT
GACTGGTTTTCTGGACTCATGAGTTGGTTTGGAGGGCCTCTTAAAACTATACTCCTCATTTGCCTGTATG
TTGCATTATCAATTGGGCTCTTTTTCCTCCTTATATATCTTGGAGGAACAGGCCTCTCTAAAATGTGGCT
TGCTGCCACTAAGAAGGCCTCATA

Protein Information

Protein Name

M protein

NCBI Protein GI

27881464

Protein Accession

NP_777376

Protein pI

7.46

Protein Weight

122468.33

Protein Length

1197

Protein Sequence

>NP_777376.1 M protein [Rift Valley fever virus]
MYVLLTILTSVLVCEAIIRVSLSSTREETCFGDSTNPEMIEGAWDSLREEEMPEELSCSISGIREVKTSS
QELYRALKAIIAADGLNNITCHGKDPEDKISLIKGPPHKKRVGIVRCERRRDAKQIGRKTMAGIAMTVLP
ALAVFALAPVVFAEDPHLRNRPGKGHNYIDGMTQEDATCKPVTYAGACSSFDVLLEKGKFPLFQSYAHHR
TLLEAVHDTIIAKADPPSCDLLSAHGNPCMKEKLVMKTHCPNDYQSAHHLNNDGKMASVKCPPKYELTED
CNFCRQMTGASLKKGSYPLQDLFCQSSEDDGSKLKTKMKGVCEVGVQALKKCDGQLSTAHEVVPFAVFKN
SKKVYLDKLDLKTEENLLPDSFVCFEHKGQYKGTMDSGQTKRELKSFDISQCPKIGGHGSKKCTGDAAFC
SAYECTAQYANAYCSHANGSGIVQIQVSGVWKKPLCVGYERVVVKRELSAKPIQRVEPCTTCITKCEPHG
LVVRSTGFKISSAVACASGVCVTGSQSPSTEITLKYPGISQSSGGDIGVHMAHDDQSVSSKIVAHCPPQD
PCLVHDCIVCAHGLINYQCHTALSAFVVVFVFSSIAIICLAILYRVLKCLKIAPRKVLNPLMWITAFIRW
IYKKMVARVADNINQVNREIGWMEGGQLVLGNPAPIPRHAPIPRYSTYLMLLLIVSYASACSELIQASSR
ITTCSTEGVNTKCRLSGTALIRAGSVGAEACLMLKGVKEDQTKFLKLKTVSSELSCREGQSYWTGSFSPK
CLSSRRCHLVGECHVNRCLSWRDNETSAEFSFVGESTTMRENKCFEQCGGWGCGCFNVNPSCLFVHTYLQ
SVRKEALRVFNCIDWVHKLTLEITDFDGSVSTIDLGASSSRFTNWGSVSLSLDAEGISGSNSFSFIESPG
KGYAIVDEPFSEIPRQGFLGEIRCNSESSVLSAHESCLRAPNLISYKPMIDQLECTTNLIDPFVVFERGS
LPQTRNDKTFAASKGNRGVQAFSKGSVQADLTLMFDNFEVDFVGAAVSCDAAFLNLTGCYSCNAGARVCL
SITSTGTGSLSAHNKDGSLHIVLPSENGTKDQCQILHFTVPEVEEEFMYSCDGDERPLLVKGTLIAIDPF
DDRREAGGESTVVNPKSGSWNFFDWFSGLMSWFGGPLKTILLICLYVALSIGLFFLLIYLGGTGLSKMWL
AATKKAS

Epitope Information

IEDB Linear Epitope