VIOLIN: Vaccine Investigation and Online Information Network

SREHP

General Information

Protegen ID

793

Sequence Strain (Species/Organism)

Entamoeba histolytica HM-1:IMSS

VO ID

VO_0011324

Taxonomy ID

294381

Molecule Role

Protective antigen

Molecule Role Annotation

Report describes a recombinant fusion protein containing the serine-rich Entamoeba histolytica protein (SREHP), a protective antigen derived from virulent amebae, and a bacterially derived maltose-binding protein (MBP) from an attenuated strain of Salmonella typhimurium. Gerbils vaccinated with S typhimurium SREHP-MBP were protected against amebic liver abscess after challenge with E. histolytica, the most common extraintestinal complication of amebiasis (Zhang and Stanley, 1996).

Related Vaccines(s)

E. histolytica SREHP protein vaccine

References

Zhang and Stanley, 1996: Zhang T, Stanley SL Jr. Oral immunization with an attenuated vaccine strain of Salmonella typhimurium expressing the serine-rich Entamoeba histolytica protein induces an antiamebic immune response and protects gerbils from amebic liver abscess. Infection and immunity. 1996; 64(5); 1526-1531. [PubMed: 8613356].

Gene Information

Gene Name

SREHP

NCBI Gene ID

3402462

Genbank Accession

DS571976

Chromosome No

Unknown

Locus Tag

EHI_116360

Gene Starting Position

1107

Gene Ending Position

1833

DNA Sequence

>NW_001915691.1:1107-1833 Entamoeba histolytica HM-1:IMSS scf_1104750518053 genomic scaffold, whole genome shotgun sequence
AGACTAAAGTCAAATAACTTCATTTAGAAGATGATAGCTATAATGATAGCACAGAAAACAATGAATGGAC
TTGATGCAGCATCAAGGTTATTATTATCGTTATCTGAACTACTTCCTGATTTATCATTTGAATTACTAGT
TGAGCTTGCTTCTGGTTTATTAGTTGAGCTTGCTTCTGGTTTATTAGTTGAGCTTGCTTCTGGTTTATTA
GTTGAGCTTGCTTCTGGTTTATTAGTTGAGCTTGCTTCTGGTTTATTATCTGGTTTATCACTTGAGCTTG
CTTCTGGTTTATTATCTGGTTTATCACTTGAGCTTGCTTCTGGTTTATTATCTGGTTTATCACTTGAACT
TGCTTCTGGTTTATTATCTGGTTTATCACTTGAACTTGCTTCTGGTTTATTATCTGGTTTATCACTTGAA
CTTGCTTCTGGTTTATCACTTGAACTTGCTTCTGGTTTATTATCTGGTTTATCACTTGAGCTTGATTCTG
AGTTATCACTTGAACTTGCTTTTTCATCTTCATCATCTTCATTATCTTCATTTGAACTTGATTCTGGTTT
TGCTGAGCTACTTTTTTCTTTTTCTTCAGCTTCTTCAAGCTTTTCAGGACTAGCTTCGTTCTTTAAAAAT
ACACCGTAGATATTAGTATCTTTTACTTCTTGATCCAAATCAAGAATGATATTAGTTGCACTAGTGAATG
CAATAAACAATAAAAATGCGAACATTG

Protein Information

NCBI Protein GI

67463194

Protein Accession

XP_648254

Protein pI

4.01

Protein Weight

24419.56

Protein Length

233

Protein Note

transcript EHI_116360A

Protein Sequence

>XP_648254.1 serine-rich protein [Entamoeba histolytica HM-1:IMSS]
MFAFLLFIAFTSATNIILDLDQEVKDTNIYGVFLKNEASPEKLEEAEEKEKSSSAKPESSSNEDNEDDED
EKASSSDNSESSSSDKPDNKPEASSSDKPEASSSDKPDNKPEASSSDKPDNKPEASSSDKPDNKPEASSS
DKPDNKPEASSSDKPDNKPEASSTNKPEASSTNKPEASSTNKPEASSTNKPEASSTSNSNDKSGSSSDND
NNNLDAASSPFIVFCAIIIAIIF

Epitope Information

IEDB Linear Epitope