VIOLIN: Vaccine Investigation and Online Information Network

vacA

General Information

Protegen ID

810

Sequence Strain (Species/Organism)

Helicobacter pylori

VO ID

VO_0011341

Taxonomy ID

210

Other Database IDs

CDD:111576
CDD:309066

Molecule Role

Protective antigen

Molecule Role Annotation

Treatment of the Helicobacter pylori vacuolating cytotoxin with very low concentrations of formaldehyde resulted in abrogation of toxic activity in both a HeLa cell vacuolation assay and an in vivo assay of gastric epithelial damage. Detoxification had only a minimal effect on the integrity of the oligomeric or monomeric structure. The toxoid retained the ability to bind to target cells and to induce high-titer neutralizing antibodies after immunization of rabbits. Furthermore, oral immunization of mice with the toxoid resulted in protection against infective challenge with mouse-adapted strains of H. pylori (Manetti et al., 1997).

Related Vaccines(s)

H. pylori VacA protein vaccine

References

Manetti et al., 1997: Manetti R, Massari P, Marchetti M, Magagnoli C, Nuti S, Lupetti P, Ghiara P, Rappuoli R, Telford JL. Detoxification of the Helicobacter pylori cytotoxin. Infection and immunity. 1997; 65(11); 4615-4619. [PubMed: 9353041].

Gene Information

Gene Name

vacA

Protein Information

Protein Name

autotransporter vacuolating cytotoxin VacA

NCBI Protein GI

WP_123946465

Protein pI

9.4

Protein Weight

132293.35

Protein Length

1391

Protein Note

Vacuolating cyotoxin; pfam02691

Protein Sequence

>WP_123946465.1 autotransporter vacuolating cytotoxin VacA [Helicobacter pylori]
MEIQQTHRKINRPLVSLVLAGALISAIPQQSHAAFFTTVIIPAIVGGIATGTAVGTVSGLLGWGLKQAEE
ANKTPDKPDKVWRIQAGKGFNEFPNKEYDLYRSLLSSKIDGGWDWGNAATHYWVKGGQQNKLEVDMKDAV
GTYKLSGLRNFTGGDLDVNMQKATLRLGQFNGNSFTSFKDSADRTTRVDFNAKNISIDNFVEINNRVGSG
AGRKASSTVLTLQASEGITSSKNAEISLYDGATLNLASNSVKLNGNVWMGRLQYVGAYLAPSYSTINTSK
VTGEVDFNHLTVGDKNAAQAGIIASNKTHIGTLDLWQSAGLNIIAPPEGGYKDKPKDKPSNTTQNNANNN
QQNSAQNNSSTQVINPPNSAQKTEIQPTQVIDGPFAGGKDTVVNIDRINTKADGTIKVGGYKASLTTNAA
HLHIGKGGVNLSNQASGRTLLVENLTGNITVDGPLRVNNQVGGYALAGSSANFEFKAGTDTKNGTATFNN
DISLGRFVNLKVDAHTANFKGIDTGNGGFNTLDFSGVTGKVNINKLITASTNVAAKNFNINELIVKTNGV
SVGEYTHFSEDIGSQSRINTVRLETGTRSIFSGGVKFKSGEKLVIDEFYYSPWNYFDARNIKNVEITRKF
ASSTPENPWGTSKLMFNNLTLGQNAIMDYSQFSNLTIQGDFINNQGTINYLVRGGQVATLNVGNAAAMMF
NNDIDSATGFYKPLIKINSAQDLIKNTEHVLLKAKIIGYGNVSTGTNGISNVNLEEQFKERLALYNNNNR
MDTCVVRNTDDIKACGMAIGNQSMVNNPDNYKYLIGKAWKNIGISKTANGSKISVYYLGNSTPTENGGST
TNLPTNTTNNARSANYALVKNAPFAHSATPNLVAINQHDFGTIESVFELANRSKDIDTLYTHSGAQGRDL
LQTLLIDSHDAGYARTMIDATSANEITKQLNAATTTLNNIASLEHKTSGLQTLSLSNAMILNSRLVNLSR
RHTNHIDSFAQRLQALKDQRFASLESAAEVLYQFAPKYEKPTNVWANAIGGASLNNGSNASLYGTSAGVD
AFLNGNVEAIVGGFGSYGYSSFSNQANSLNSGANNANFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKS
ALLQDLNQSYNYLAYSATARASYGYDFAFFRNALVLKPSVGVGYNHLGSTNFKSNSQSQVALKNGASSQH
LFNANANVEARYYYGDTSYFYMNAGVLQEFANFGSSNAVSLNTFKVNATHNPLNTHARVMMGGELKLAKE
VFLNLGFVYLHNLISNIGHFASNLGMRYSF

Epitope Information

IEDB Linear Epitope