VIOLIN: Vaccine Investigation and Online Information Network

PPE14

General Information

Protegen ID

822

Sequence Strain (Species/Organism)

Mycobacterium tuberculosis H37Rv

VO ID

VO_0012374

Taxonomy ID

83332

Molecule Role

Protective antigen

Molecule Role Annotation

immunization with the soluble recombinant rMTB41 protein plus adjuvant, a regimen known to generate primarily CD4+ T cells, resulted in induction of protection comparable to BCG in two well-established animal models of tuberculosis (mice and guinea pigs) (Skeiky et al., 2005).

References

Skeiky et al., 2005: Skeiky YA, Alderson MR, Ovendale PJ, Lobet Y, Dalemans W, Orme IM, Reed SG, Campos-Neto A. Protection of mice and guinea pigs against tuberculosis induced by immunization with a single Mycobacterium tuberculosis recombinant antigen, MTB41. Vaccine. 2005; 23(30); 3937-3945. [PubMed: 15917115].

Gene Information

Gene Name

PPE14

NCBI Gene ID

885069

Genbank Accession

AL123456

Locus Tag

Rv0915c

Gene Starting Position

1020057

Gene Ending Position

1021328

DNA Sequence

>NC_000962.3:1020057-1021328 Mycobacterium tuberculosis H37Rv, complete genome
TTTACCGCGGGGGGTTTCCGGGCGGCGGCTGCTCTCTAATCACAACTACCGGGGGTTTGCGGCCGTCCTC
TTGGCCGTCAGTGCTGGTGCCGCTACGGGTGCCGCCACCGCCCGTCGTGCCGCGTGCGGCCAGGCTCGCC
AAAGCCATCCCGCTGAGCAGGCCTGCCGGCATCCCGTTTAGGGCCGTCGGGTCGGCGCCGGCGCTGGAGC
TGAAGGTGGGTGTTGCCTGAACGGCGAGCTGGATCTCCGGGGCGGCCGTGGTCCAGCTGTGCGGCACCGA
CAACGCTCCGACTAATGCTGCGTGGCCGACGCCCGCGGACACCGGCGCCGCGCCCCCGAAGGGGCCCCAG
TGCGGCTCCGGCTCGTCGGTCGCCGAACTCAGTGGATGGCCCTGCGTCGGTCCCAGCCCGCCGGCGTTCC
CGTATAGGCCGATGTGCCAGGGTCTGGCCGTGTTCGTGATCGCGAGCGCAATGCTGCCGGTCGCGATGGA
TGCAATGTAGAGCGCGATCACGTCCAATTCCCCTATCGGGGTGGGGATCACTATCGGCTGAGCGGATCCG
ACTTGCGGGTTGAGGGTCGACGCGATCCCCAACAGTCCCGATGTCAGCGGATCAGCGTTGGCGGCCAATG
CGGACAGAATGTCGCTCAGGATCCCCGGGGGCAGCTGGGCCAGTGTCGCCTGTGCATCCGCAACGGCGCC
CGCACCGGCGGCTTGGGTCGCCGCGGCTGCGGCCGCGGGCCCGGCCGGGCCGGTGCCTTGCACGGGTGGA
GTGAACGGCGGCAACGCCGACGCGGCCGCAGATGCCCCCTCATAGCTGTACATCACGGCAGCGTCTTGGG
CCCACATTTCGGCATACTCGGCCTGGGTAGCCGCGATCGCCGCACTGTTTTGCCCCAGAATGTTCGCCGC
GACCAGCGACATCAACCGGCTGCGGTTGGCCGCGACGAGGGATGGTGGCACCGTCATCGCGAACGCCGTC
CCAAACGCTTCCGCCGCTGCCCTCGCCTGTGTGGCCGTCTCCTTCGCCAGCGCCGCCGTGGCGGCCAGCC
ACCCCACATACGGCGTTGCCGCGGCCGCCATCGCGGCCGCCGCCGGCCCCATCCACGGCTCAACGATCAG
CGTCGACACCACCGATCCATACGAGACCGCGGCGGAAGTCAACTCCGCGGCCACACCGTCCCAGGCGGCC
GCGGCGGCTAGCATCGACTCCGGCCCCGGACCGGAATACATTCGGCTTGAATTCACTTCCGGAGGTAAAA
GCCCGAAATCCA

Protein Information

Protein Name

PPE family protein PPE14

NCBI Protein GI

57116795

Protein Accession

YP_177765

Protein pI

4.31

Protein Weight

39057.75

Protein Length

423

Protein Note

Rv0915c, (MTCY21C12.09c), len: 423 aa. PPE14 (alternate gene name: MTB41). Member of the Mycobacterium tuberculosis PPE family (see citation below), highly similar to many e.g. Rv1807 from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 966, E(): 4.4e-30, (45.7% identity in 392 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2.; MTB41

Protein Sequence

>YP_177765.1 PPE family protein PPE14 [Mycobacterium tuberculosis H37Rv]
MDFGLLPPEVNSSRMYSGPGPESMLAAAAAWDGVAAELTSAAVSYGSVVSTLIVEPWMGPAAAAMAAAAT
PYVGWLAATAALAKETATQARAAAEAFGTAFAMTVPPSLVAANRSRLMSLVAANILGQNSAAIAATQAEY
AEMWAQDAAVMYSYEGASAAASALPPFTPPVQGTGPAGPAAAAAATQAAGAGAVADAQATLAQLPPGILS
DILSALAANADPLTSGLLGIASTLNPQVGSAQPIVIPTPIGELDVIALYIASIATGSIALAITNTARPWH
IGLYGNAGGLGPTQGHPLSSATDEPEPHWGPFGGAAPVSAGVGHAALVGALSVPHSWTTAAPEIQLAVQA
TPTFSSSAGADPTALNGMPAGLLSGMALASLAARGTTGGGGTRSGTSTDGQEDGRKPPVVVIREQPPPGN
PPR

Epitope Information

IEDB Linear Epitope