VIOLIN: Vaccine Investigation and Online Information Network

DnaK

General Information

Protegen ID

866

Sequence Strain (Species/Organism)

Mycobacterium tuberculosis H37Rv

VO ID

VO_0012408

Taxonomy ID

83332

Molecule Role

Protective antigen

Molecule Role Annotation

In an ex vivo transfection approach with a retroviral vector a single antigen (hsp70) could evoke strong protection in mice (Lowrie et al., 1997).

Related Vaccines(s)

BCG-DHTM

References

Lowrie et al., 1997: Lowrie DB, Silva CL, Colston MJ, Ragno S, Tascon RE. Protection against tuberculosis by a plasmid DNA vaccine. Vaccine. 1997; 15(8); 834-838. [PubMed: 9234527].

Gene Information

Gene Name

DnaK

NCBI Gene ID

885946

Genbank Accession

AL123456

Locus Tag

Rv0350

Gene Starting Position

419834

Gene Ending Position

421711

DNA Sequence

>NC_000962.3:419834-421711 Mycobacterium tuberculosis H37Rv, complete genome
CATGGCTCGTGCGGTCGGGATCGACCTCGGGACCACCAACTCCGTCGTCTCGGTTCTGGAAGGTGGCGAC
CCGGTCGTCGTCGCCAACTCCGAGGGCTCCAGGACCACCCCGTCAATTGTCGCGTTCGCCCGCAACGGTG
AGGTGCTGGTCGGCCAGCCCGCCAAGAACCAGGCAGTGACCAACGTCGATCGCACCGTGCGCTCGGTCAA
GCGACACATGGGCAGCGACTGGTCCATAGAGATTGACGGCAAGAAATACACCGCGCCGGAGATCAGCGCC
CGCATTCTGATGAAGCTGAAGCGCGACGCCGAGGCCTACCTCGGTGAGGACATTACCGACGCGGTTATCA
CGACGCCCGCCTACTTCAATGACGCCCAGCGTCAGGCCACCAAGGACGCCGGCCAGATCGCCGGCCTCAA
CGTGCTGCGGATCGTCAACGAGCCGACCGCGGCCGCGCTGGCCTACGGCCTCGACAAGGGCGAGAAGGAG
CAGCGAATCCTGGTCTTCGACTTGGGTGGTGGCACTTTCGACGTTTCCCTGCTGGAGATCGGCGAGGGTG
TGGTTGAGGTCCGTGCCACTTCGGGTGACAACCACCTCGGCGGCGACGACTGGGACCAGCGGGTCGTCGA
TTGGCTGGTGGACAAGTTCAAGGGCACCAGCGGCATCGATCTGACCAAGGACAAGATGGCGATGCAGCGG
CTGCGGGAAGCCGCCGAGAAGGCAAAGATCGAGCTGAGTTCGAGTCAGTCCACCTCGATCAACCTGCCCT
ACATCACCGTCGACGCCGACAAGAACCCGTTGTTCTTAGACGAGCAGCTGACCCGCGCGGAGTTCCAACG
GATCACTCAGGACCTGCTGGACCGCACTCGCAAGCCGTTCCAGTCGGTGATCGCTGACACCGGCATTTCG
GTGTCGGAGATCGATCACGTTGTGCTCGTGGGTGGTTCGACCCGGATGCCCGCGGTGACCGATCTGGTCA
AGGAACTCACCGGCGGCAAGGAACCCAACAAGGGCGTCAACCCCGATGAGGTTGTCGCGGTGGGAGCCGC
TCTGCAGGCCGGCGTCCTCAAGGGCGAGGTGAAAGACGTTCTGCTGCTTGATGTTACCCCGCTGAGCCTG
GGTATCGAGACCAAGGGCGGGGTGATGACCAGGCTCATCGAGCGCAACACCACGATCCCCACCAAGCGGT
CGGAGACTTTCACCACCGCCGACGACAACCAACCGTCGGTGCAGATCCAGGTCTATCAGGGGGAGCGTGA
GATCGCCGCGCACAACAAGTTGCTCGGGTCCTTCGAGCTGACCGGCATCCCGCCGGCGCCGCGGGGGATT
CCGCAGATCGAGGTCACTTTCGACATCGACGCCAACGGCATTGTGCACGTCACCGCCAAGGACAAGGGCA
CCGGCAAGGAGAACACGATCCGAATCCAGGAAGGCTCGGGCCTGTCCAAGGAAGACATTGACCGCATGAT
CAAGGACGCCGAAGCGCACGCCGAGGAGGATCGCAAGCGTCGCGAGGAGGCCGATGTTCGTAATCAAGCC
GAGACATTGGTCTACCAGACGGAGAAGTTCGTCAAAGAACAGCGTGAGGCCGAGGGTGGTTCGAAGGTAC
CTGAAGACACGCTGAACAAGGTTGATGCCGCGGTGGCGGAAGCGAAGGCGGCACTTGGCGGATCGGATAT
TTCGGCCATCAAGTCGGCGATGGAGAAGCTGGGCCAGGAGTCGCAGGCTCTGGGGCAAGCGATCTACGAA
GCAGCTCAGGCTGCGTCACAGGCCACTGGCGCTGCCCACCCCGGCGGCGAGCCGGGCGGTGCCCACCCCG
GCTCGGCTGATGACGTTGTGGACGCGGAGGTGGTCGACGACGGCCGGGAGGCCAAGTG

Protein Information

Protein Name

chaperone protein DnaK

NCBI Protein GI

15607491

Protein Accession

NP_214864

Protein pI

4.59

Protein Weight

61176.37

Protein Length

625

Protein Note

heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria

Protein Sequence

>NP_214864.1 chaperone protein DnaK [Mycobacterium tuberculosis H37Rv]
MARAVGIDLGTTNSVVSVLEGGDPVVVANSEGSRTTPSIVAFARNGEVLVGQPAKNQAVTNVDRTVRSVK
RHMGSDWSIEIDGKKYTAPEISARILMKLKRDAEAYLGEDITDAVITTPAYFNDAQRQATKDAGQIAGLN
VLRIVNEPTAAALAYGLDKGEKEQRILVFDLGGGTFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVD
WLVDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINLPYITVDADKNPLFLDEQLTRAEFQR
ITQDLLDRTRKPFQSVIADTGISVSEIDHVVLVGGSTRMPAVTDLVKELTGGKEPNKGVNPDEVVAVGAA
LQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTRLIERNTTIPTKRSETFTTADDNQPSVQIQVYQGERE
IAAHNKLLGSFELTGIPPAPRGIPQIEVTFDIDANGIVHVTAKDKGTGKENTIRIQEGSGLSKEDIDRMI
KDAEAHAEEDRKRREEADVRNQAETLVYQTEKFVKEQREAEGGSKVPEDTLNKVDAAVAEAKAALGGSDI
SAIKSAMEKLGQESQALGQAIYEAAQAASQATGAAHPGGEPGGAHPGSADDVVDAEVVDDGREAK

Epitope Information

IEDB Linear Epitope