VIOLIN: Vaccine Investigation and Online Information Network

MIC8

General Information

Protegen ID

876

Taxonomy ID

5811

Molecule Role

Protective antigen

Molecule Role Annotation

The gene sequence encoding MIC8 was inserted into the eukaryotic expression vector pVAX I, and the pVAX-MIC8 expression plasmid was constructed, and the plasmid diluted with PBS to 100 mg/100 microl was injected into the Kunming mice muscularly. Levels of IgG antibody, gamma-interferon (IFN-gamma), interleukin-2 (IL-2), interleukin-4, and interleukin-10 were detected. The mice were challenged with tachyzoites of the virulent T. gondii RH strain at the 14th day after the last immunization to observe the survival time. The high level of IFN-gamma, IL-2, and IgG antibody indicated that mice vaccinated with recombinant pVAX-MIC8 plasmid could elicit strong cellular and humoral immune responses and showed a significantly increased survival time (10.3 +/- 0.9 days) compared with control mice which died within 5 days of challenge infection (Liu et al., 2010).

Related Vaccines(s)

T. gondii DNA vaccine encoding MIC8

References

Liu et al., 2010: Liu MM, Yuan ZG, Peng GH, Zhou DH, He XH, Yan C, Yin CC, He Y, Lin RQ, Song HQ, Zhu XQ. Toxoplasma gondii microneme protein 8 (MIC8) is a potential vaccine candidate against toxoplasmosis. Parasitology research. 2010; 106(5); 1079-1084. [PubMed: 20177910].

Gene Information

Gene Name

MIC8

NCBI Gene ID

7899500

NCBI Nucleotide GI

13346486

DNA Sequence

>gi|13346486|gb|AF353165.1| Toxoplasma gondii microneme protein 8 (MIC8) gene, complete cds
ATGAAGGCCAATCGAATATGGTGTTTTTTTGCGTGGCGTATGGTTGTGCGGGCCTCATTTCTGAAAGAGA
TGGACAGCATTTTCGTTTCTGCTATCCGACAGAATGTACAGCATACTCATTCTGCCCTTCTCGCCAAACT
GAAGGAACCCCCAGATCCAGATGATGAGAACTCTTGGCTTTGTCGAATATCAAAAAAATATGACGCATGC
GGTAGCAGGGAATATTCCGATAAGGGCCTCAAAGGGACGTACTGTCCCGAGGATTTTTGCTGTAGCAAGA
CGGCATGTTTTTACGGTTCATGTGGGAGTTGGTGCCACGACAACTGGGCTCTGTGCAGCTCATCTATAAT
CTACCACGACGAGTACAGTTACGGGAAATGCAACTGTAAACGGTTTCAAGAAAACTGTGATGTGAATGCA
ATTTGTGTGCATGCGAACAGAGAGGATGGCGGTGCGTATTGTCAGTGCAAGGAAGGATATTGGGGTGATG
GTAAATCGTGCAAGATTGACTTCTGCCAACTGCAGCCCTGTGGTGCAGGGACCTGCACCAGGACGGATGA
AGGATACAAGTGTGATTGCCCAGAAACTCACAAGCTTATTGTCGTTGAAGACAAAGAGACGTGCAAGGCA
AAACCGGACTTTTGCGCGGAAGAGCCTTGCGGACCACCCTCTATGGTTGAAAATTGCGTGAACACCGATG
ACAGCTACGAATGTGTTTGCAAACAGGGGTATGAAGTGAGGAACGGTCGGTGCGAAGAAATTGACTTATG
CGCGGACAAGCCATGTGGGCCAGATGAGGGTGTGCATGAGTGTGTAACAGAGAGGCAACCGAAATTAAGG
TACAGATGCACGTGCAAGGCAGGATTCGATTTGACCACCTTGCCTGATGGTGTTTCCCAGAAGTGCCTGA
AGAACTTCTGTTACGAGGAGCCCTGCGGCACCCGGGACCTAGTTGAAAGCTGTAAGTCAAAGGCATATGG
ATACTCGTGTTTGTGTGCGGCAGGTGCCATGGTTCAAGTGATTAACGGAAAAGAAAAGTGCATCAAGGCG
GACTTGTGCCGCAATGATCCGTGTGGTCCAGAGACAGCAGTGATTCAATGTTACTCTCATGGCACCAGCT
ATAGGTGTTTGTGCAAAGCAGGCTACACTGAAGTTTTTGTTAACGGGAAGAGTTCATGTCAAAAGGGCGA
CCCATGCACTCTGAACATGTGTGGCGGTAACGAAGCGGTCCAGGAGTGCACAACTGATGGCACGGCGTAC
GGGTGTACCTGCAAGCCAGGCTATTCGATAGCCATTAAGCATGGTCAGAAGTTTTGCAACCCTGAAGAGG
AGTGTGCTTCTCATTGTGGCTCGGCAGCTGCAGTGAAAAGCTGTGAAATACTTGACTCTGGCGGATACCA
GTGTACATGCAATCCAGGATACGTCATGAGATACAGCGACTATGTAAAAGGATGCGTCGAGGGAAATCAG
TGTTCTCTCAATCCTTGTGGGGAGCAGGAAGCCGTGCAAAGGTGCATTCCTGAAGGTGACACGTATGATT
GCGAGTGCAATCCGGGGTTCGTCAAAAGAGTCTTGCCGGATGGGAATTTCATTTGCGCCGATCCAGCGAG
CTGTGTAGGGAATCCCTGTGGTAGCTCAGATGCGGTCGATGCGTGCATTGCCGGGACTAGCACGTATACA
TGCAGGTGTAAGGACGGATACACACCTCAGTCAATTGGGTCAAAGTTGCAGTGTTTACCAGAAAGCACTG
ATCAGACAGATTTCGATTCCAAACACAAACCAGAGGACAACAAAGGTCGATATTCGAAAGGAACAATTGC
ATTGGTGGTTGTGGGGTGTGTAGCCTTGTTGGGTATTATAGCCGGAGGAATTTCTTACGCCAGAAACAGA
GGAGGTGAGCGCGATGATGAAGACTTGGCACCACCACCTCGTTCCACACGAGAACGGAGGCTCTCATCAA
TGGGCGAAGGTTTTGAGAATGCCTCATGGGCATCTTCTGTAAGTATGATTCCTAGTGCACCTGCTCCGCC
ACCTTCGGGCGGTATCTGGTCCTAA

Protein Information

Protein Name

microneme protein 8

NCBI Protein GI

13346487

Protein Accession

AAK19757.1

Protein pI

5.13

Protein Weight

71329.71

Protein Length

747

Protein Sequence

>AAK19757.1 microneme protein 8 [Toxoplasma gondii]
MKANRIWCFFAWRMVVRASFLKEMDSIFVSAIRQNVQHTHSALLAKLKEPPDPDDENSWLCRISKKYDAC
GSREYSDKGLKGTYCPEDFCCSKTACFYGSCGSWCHDNWALCSSSIIYHDEYSYGKCNCKRFQENCDVNA
ICVHANREDGGAYCQCKEGYWGDGKSCKIDFCQLQPCGAGTCTRTDEGYKCDCPETHKLIVVEDKETCKA
KPDFCAEEPCGPPSMVENCVNTDDSYECVCKQGYEVRNGRCEEIDLCADKPCGPDEGVHECVTERQPKLR
YRCTCKAGFDLTTLPDGVSQKCLKNFCYEEPCGTRDLVESCKSKAYGYSCLCAAGAMVQVINGKEKCIKA
DLCRNDPCGPETAVIQCYSHGTSYRCLCKAGYTEVFVNGKSSCQKGDPCTLNMCGGNEAVQECTTDGTAY
GCTCKPGYSIAIKHGQKFCNPEEECASHCGSAAAVKSCEILDSGGYQCTCNPGYVMRYSDYVKGCVEGNQ
CSLNPCGEQEAVQRCIPEGDTYDCECNPGFVKRVLPDGNFICADPASCVGNPCGSSDAVDACIAGTSTYT
CRCKDGYTPQSIGSKLQCLPESTDQTDFDSKHKPEDNKGRYSKGTIALVVVGCVALLGIIAGGISYARNR
GGERDDEDLAPPPRSTRERRLSSMGEGFENASWASSVSMIPSAPAPPPSGGIWS

Epitope Information

IEDB Linear Epitope