VIOLIN: Vaccine Investigation and Online Information Network

isdB

General Information

Protegen ID

893

Sequence Strain (Species/Organism)

Staphylococcus aureus subsp. aureus str. Newman

Taxonomy ID

426430

Other Database IDs

CDD:213844

Molecule Role

Protective antigen

Molecule Role Annotation

In preclinical studies, a isdB vaccine has shown protection from lethal infection in mice (Harro et al., 2010).
Iron-regulated surface determinant B (IsdB) of Staphylococcus aureus (S. aureus) is a highly conserved surface protein that can induce protective CD4(+) T-cell immune response. A pivotal role of CD4(+) T-cells in effective immunity against S. aureus infection has been proved(Yu et al., 2015)

References

Harro et al., 2010: Harro C, Betts R, Orenstein W, Kwak EJ, Greenberg HE, Onorato MT, Hartzel J, Lipka J, DiNubile MJ, Kartsonis N. Safety and immunogenicity of a novel Staphylococcus aureus vaccine: results from the first study of the vaccine dose range in humans. Clinical and vaccine immunology : CVI. 2010; 17(12); 1868-1874. [PubMed: 20943877].

Yu et al., 2015: Yu S, Zhang H, Yao D, Liu W, Wang X, Chen X, Wei Y, Zhang Z, Wang J, Yu L, Sun H, Wu Z, Yu Y, Song B, Ma J, Tong C, Cui Y. Identification of CD4+ T-cell epitopes on iron-regulated surface determinant B of Staphylococcus aureus. Microbial pathogenesis. 2015; 89; 108-113. [PubMed: 26423555].

Gene Information

Gene Name

isdB

NCBI Gene ID

5330644

Genbank Accession

AP009351

Locus Tag

NWMN_1040

Gene Starting Position

1142079

Gene Ending Position

1144016

DNA Sequence

>NC_009641.1:1142079-1144016 Staphylococcus aureus subsp. aureus str. Newman DNA, complete genome
ATTAGTTTTTACGTTTTCTAGGTAATACGAATGCAACGATGCTACTTAAAGCTAATAATGCCATTAATGG
TAATGTCATATCTTTATTTGATTCTTCACCAGTTTGTGGTAATGATTTTGCTTTATTTTCTTGTGTATTT
TTATTGTTTTGGCTTTGAGTGTGTCCATCATTTGTGTTTTTAATGTTTGCTTTTTGTAATGGAGCACTAT
CTTTTGCTTCGCTAGAACCTGCTGAAGTTTGAACAACATCTTTTGTTGTTTTTGATGAAGCAGTTGTTGG
TTTTGCAACATTTTGAGTCGTAGATACTACCTTAGTTGGAGTTGTACTACTTGATTCTACTTCACCTTTA
GTTGGTTTTGTAGCAGGCGTTTTGTCTTTACCTGACTCACTAGATGCGTCATTTTCTTTTTCAACACTTG
GTAATTGTTTATTGTCATCTTTTTGGCTGTCTTGTTTTTGTGATTCTTTTTCAACAGGTGATGGTGTTGG
TTTGCTAGGCGTAGCTGGAGTAGCTTCCTTCTTAGCTGAGTTATCTTGTTGTTCTTTTTTGTTAGATTTA
TCGGTATTGGCTTTTGTAAATGCTTCTTTATCAACGATTCTGACATGGTATTGTCCATCATAATCAATCG
TTTTTACGTGAACTTTAACGATAGCATCATATAGAGTTTTACCTTCAACATATGGGAAAATAATTGTTCT
AGTATTATTTTTAGCATCTTTGCTTATAGTTCTAACACGTTGACCTTCAACCATGAAATCTTTCCAGTAA
TCGTCATTAGTAGTTTCCATGACCATATATTTTTTGCCGTTAAGCATACCTGTTTTAATAGGGTGTTTAA
CAAAAGTATCCATCATAGATTCGTTATTCTCAACACTTTCATAAACAACATATTTTGTATCTTGTAAATC
AGTCATTTTTTCATTTGTTGGTTGTACATTTTGGAATTCAGTAATAGCTGATTTCACTTGCTCATCTAAA
GCTTTCTTTGTATCCTCTAATTTCTTCTTGTACTCAGCCTTTAATTTTTCAGGAAGTTTATCTTGAATTT
TATTTAATTCATAAACTTGTCTTTCTAGTGTTTTCGCTTTTTTATATGGCGCTAATAATTTTTCAGCTTT
ATAATCTTCTTCAGTTTTGAATTTATCTGCACTGTTATAAATTGGTTGTGCGAATTCCATTAATGTGTAA
TCGTATTTTTCTTCTTTGTTATTGAAGTGTGTTGAACTAACAATTTTAACAGCTTTTGTTCCGTTTGATA
CAGAGAAGCGAATGTAAGCATAATCTTTAACAGTATCGTATGATACTAATTTAATTGGCAACTTTTTGTC
ACCTTCATAAACTTCAAATTTTCTCCAAAATTGACCTGATTGTAATCCTAATTCAATTTCTGGTTTTGAA
TCAGTGAAAATAACTCTAGCAGGTTTAACAGAACTTGCATAATGATAAAACTGTTGAGTTCCATCTTTCT
TTTTCATTTCAAAATCAATTGGACGAGAGTTTGGTGCGCTATGATCTTTGTCTTTTATTGCAGGGTTTTT
AATCGCTTCTCTAAGTTCCTGATTCAAAATAGGATATGTATTGTTAGTGGCTTTTGCTGCTGGTTTAACT
TCTTTTGTTTCCTTAGGGGCTTTAACTTCTTTAACTTCTTTAGCTTCTTTTGTTTCAGAAGTAGGGGCCT
CAACTTCTTTATTAGATACTGAGACAGCATTAGCTACTGGTTTAGTTTCTGGAGCTTTTTCAGATGTTGT
TGTTGGACTTGCAACTGCTTCAGTTTTTGGTTGTGCTTCTGTATTTGTACCACCTGTTTCTTCAGCTGCT
GCTTGTGCTTCGCCATTTGACATTAATAATAAAAGTGTACTAATTGCTACAGATGCAACGCCTAGTGATG
ACTTTCTAATTGAATAAAATGATTTAAATTCTTTTTGCTGTTTGTTCA

Protein Information

Protein Name

iron-regulated heme-iron binding protein IsdB

NCBI Protein GI

151221252

Protein Accession

YP_001332074

Protein pI

9.6

Protein Weight

67826.06

Protein Length

645

Protein Note

heme uptake protein IsdB; TIGR03657

Protein Sequence

>YP_001332074.1 iron-regulated heme-iron binding protein IsdB [Staphylococcus aureus subsp. aureus str. Newman]
MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEETGGTNTEAQPKTEAVASPTTTSEKAP
ETKPVANAVSVSNKEVEAPTSETKEAKEVKEVKAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKD
KDHSAPNSRPIDFEMKKKDGTQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIK
LVSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEKLLA
PYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQNVQPTNEKMTDLQDTKY
VVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPY
VEGKTLYDAIVKVHVKTIDYDGQYHVRIVDKEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVE
KESQKQDSQKDDNKQLPSVEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSK
TTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPLMALLA
LSSIVAFVLPRKRKN

Epitope Information

IEDB Linear Epitope