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eae

General Information
Protegen ID 93
Sequence Strain (Species/Organism) Escherichia coli O157:H7 str. EDL933
VO ID VO_0010944
Taxonomy ID 562
Molecule Role Protective antigen
Molecule Role Annotation Systemic immunization of calves with a combination of EspA, intimin-531, and H7 flagellin resulted in a significant reduction in shedding of EHEC O157 to levels predicted to significantly impact on EHEC O157 cattle-to-cattle transmission (McNeilly et al., 2015)
Related Vaccines(s) E. coli O157:H7 intimin vaccine , E. coli vaccine using intimin polypeptide
References
McNeilly et al., 2015: McNeilly TN, Mitchell MC, Corbishley A, Nath M, Simmonds H, McAteer SP, Mahajan A, Low JC, Smith DG, Huntley JF, Gally DL. Optimizing the Protection of Cattle against Escherichia coli O157:H7 Colonization through Immunization with Different Combinations of H7 Flagellin, Tir, Intimin-531 or EspA. PloS one. 2015; 10(5); e0128391. [PubMed: 26020530].
Gene Information
Gene Name eae
NCBI Gene ID 960862
Genbank Accession AE005174
Locus Tag Z5110
Gene Starting Position 4665440
Gene Ending Position 4668244
DNA Sequence 
>NC_002655.2:4665440-4668244 Escherichia coli O157:H7 str. EDL933 genome
ATTATTCTACACAAACCGCATAGACATTTGGAGTATTAACATTAACCCCAGGAAGAGGGTTTTGTGTTAT
TAGGTTATAAGTGCTTGATACTCCAGAACGCTGCTCACTAGATGTCTGTTTAATCCAAGCAGTTATTGAG
TTCATAGAACTATAATGGCTATATTTATTTGCAGCCCCCCATGAGTCATAAATATCTGACAATACCGTCT
GTGTGGATGGTAATAAATTTTTGCAAATGGACATAGCATCAGCATAATAGGCTTGCTTATCCACTTTTAT
CATATACGACGGTGCTTTTATAGTGTAACTTACTGTTTGCTTATCACCAGATGTGGCTTTAATTACGACA
CTGCCTTTACCATTCAAAGTGACTTTCCCTGATGCATCGACAGTCGCGATACTGGTATTTTCTGAATACC
ATGAATATGTACCATCACCACCGCTTGCTTTCAGTTTAAACTGACCATATTGCAGCCAAATATTAGGCAA
CTCGCCTCTGACATTGTTACCAATAATATCAACCTTGTTGTCAATTTTCAGTTCATCAAAAAAAGTGACC
TCAGTCGCTTTAACCTCAGCCCCATCACTGACTGTCGCACTAACAGTCGCTTTACCGGCGGAACTGGAAG
TTAGTGTTATCGTCGCACGACCATCATTTCCCGTGGTTGCTTGCGTTTGAGACTTACCGTTGAACATCCC
AAAGTTTGTTGAGAATGTAACGGATTGATTATTAACTGGCTGACCGTTTTTCATAACTTTTACAGTATAT
TTAATAGCATCCTTACCATTTGCTACTGCAGTTGTCTTATCAGCCTTAATCTCAGTAATGCTGGCCTTGG
TTTGATCAAAAAATATAACCGCACTGGCATTAAGTGCTGAAGTCATCTCCGCGGTTTTAGCAGACACGAC
GACCTGTCCTGGCGTACTCGACTTCAACGTTACGGTTGCCTTACCGTTAGCATCCGTTTTGGCACTATTT
GCCCCAAGAGTTGCAGTTCCTGAAACAATATTAAATGAAACAGGGACATTAGCCTGAGCTACCCCATTCT
TTTTCACCGTCGCGGTATAAGTAATGGTATCGGCGTTATCCGCTTTAGCCGAAGTCTTATCCGCCGTAAA
GTCCGTTACCCCAACCTGGTCGACAACTTGACCATTCGACAGAACGGTAATAGTAAGCTGTACATTGTTA
GAGCTATTGCCATTACGGTCATAGGCGCGAGCCGTCACTTTATAAATATTGCTGCCACCTTGCACATAAG
CAGGCAAAATAGCCTGGTAGTCTTGTGCGCTTTGGCTTCCGCTATGCTGAATCTGACCGCCCTGACTGCG
TAATGCACTATCATCCCAGACGATACGATCCAGACCGTATTTGCTCTTAACGATCAACTGAATCTTCTGC
GTACTGTGTTCAGTACCATTAATATCATGCGGAATATTCAGAGAAAGAATATCCTGCTTCTTGTACTCCA
GAATAATATTGTTATTACGCTGAACCAGATCGTAACGGCTGCCTGATAATGTTCTTAACTCGTTAACATA
CTGTGGTTCAATTTGCTGAGACCACGATTTATCAAACTGATAACGGAACTGCATTGAGTAAAGGAGATCA
TTTTCATTACCCGTACCATGACGGTAATCGATCCCCATCGTCACCAGAGGAATCGGAGTATAGTTTACAC
CAACGGTCGCCGCACCAGGATTCGACTGCAGCTTATCAGAATTAAACAAAGCAACATTATCACCATAATA
CTGCTCATATATCAGCTTGGCGCCTAATGCCGGATATGACGGTAGATAGCCATTAAAACGGATATCGAAG
CCATTTGCTGGGCGCTCATCATAGTCTTTCTTATTGTATGACTCATGCCAGCCGCTCATGCGGAAATAGC
CGTTAACGCTACTTTTGAAATAGTCTCGCCAGTATTCGCCACCAATACCTAAACGGGTATTATCACCAGA
AAAATCCTGATCAATGAAGACGTTATAGCCCAACATGTTTGCAGGAAGGAAAAAACGCTGACCCGCACCT
AAATTTGCCGTAAAGCGGGAGTCAATGTAACGCGCTCCGACCTGACCAAATGCCAGCATTTTTTCGGAAT
CATAGAACGGTAATAAGAAGTCCAGTGAACTACCGTCAAAGTTATTACCACTCTGCAGATTAACCTCTGC
CGTTCCATAATGTTGTAACCAGGCCTGCAACTGTGACGAAGCCTGGTTACCAGCGATACCAAGAGCGGTA
TCTTTCGCGTAATCGCCGTTCAGAGATCGCGACTGAAGCTGGCTACCGAGACTCGCCGCCTGTTGTGCCG
CATAATTTAATGCCTTGTCATCGGTCATGTTGCTTTTGGTCACGTCCGGGGACATTTTAGTCAGTTTATT
CGTGTGACCAGCAACACCACCTGCAGCAACAAGAGGTGCCGAACCTAAAAGTGGTAGTGCACTGTATTCA
AAGGGAAGTTTTTTGAGTGGCAAAATGATCTGCTGACCAGGCGCGGCCTTCATCATTTCGCTTTCAGAAC
TGTATAAATGCTTATTCAACGACCAAATCGTCGATAAATTAATATCTTGCGATTTAGAAAGATCGGCAAC
AGTTTCACCAGTTTTCAACGTATAAAAAAGGCGATTCTGATAGCTATCATGAGTTAACAGTTTTGAATCC
GAACCCAATTTAAAATAATTTTCACCATTTGCAAATGAATTCTGATTAACATAAAAAAACAATCCTAAAC
CAGCACTAAGCATAATCAATGTTTTTTTTAGCTTATGCTTGTGCCGGGTCCGGGTATAACAACCATGAGT
AATCA
Protein Information
Protein Name intimin
NCBI Protein GI WP_129935252
Protein Accession NP_290259
3D structure: PDB ID 1E5U
Protein pI 9.19
Protein Weight 95549.71
Protein Length 997
Protein Note Intimin adherence protein
Protein Sequence 
>WP_129935252.1 intimin [Escherichia coli]
MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENYFKLSSDSKLLTQNAAQDRLFYTLKTG
ETVANISKSQGISLSVIWSLNKHLYSSESEMMKAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAG
HTNKMTKMSPDATKSNTTDDKALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHY
GTAEVNLQSGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNVFIDQ
DFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYLPSYPALGAKLMY
EQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNENDLLYSMQFRYQFDKPWSQQIE
PQYVNELRTLSGSRYDLVQRNNNIILEYKKQDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDS
ALRSQGGQIQHGGSQSAQDYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGV
TDFTADKTSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTLKSATLG
QVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTVRVMKEGAPVVDQKVTFSK
DFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVGTEVKATTVEFFAPLSIDGDKVTVIGTGITG
ALPKNWLQYGQVKLQATGGNGKYTWKSSNTKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGS
IVIAVDKNTRVTYFDAENKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSNSLTAWIKQSSSEQSSG
VSSTYDLVTKNQLINVGVNNKNAFSVCVK
Vaxign Prediction
Localization(Probability) Outer Membrane
(Prob.=1)
Adhesin Probability 0.891
Trans-membrane Helices 1
Detailed Vaxign Results Vaxign Results
Epitope Information
IEDB Linear Epitope