• Vaccine Name: Influenza virus DNA vaccine encoding M1 from Influenza A virus (A/chicken/Henan/12/2004(H5N1))
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004177
• Type: DNA vaccine
• Status: Research
• Antigen: Influenza A virus (A/chicken/Henan/12/2004(H5N1)) matrix protein 1 (M1)
• M1 gene engineering:
  • Type: DNA vaccine construction
  • Description: Plasmid pCAGGSP7/M1 was constructed by cloning the PCR product M1 from the A/Chicken/Henan/12/2004(H5N1) influenza virus strain into the plasmid expression vector pCAGGSP7 (Chen et al., 2009).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005000

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Mice were immunized with plasmid DNA dissolved in 30 μl of Tris–EDTA buffer at a dosage of 50 μg by injection into the quadriceps muscles. After injection in the right quadriceps muscle, a pair of electrode needles with 5 mm apart was inserted into the muscle to cover the DNA injection sites and electric pulses were delivered using an electric pulse generator (Electro Square Porator T830 M; BTX, San Diego, CA). Three pulses of 100 V each, followed by three pulses of the opposite polarity, were delivered to each injection site at a rate of one pulse per second. Each pulse lasted for 50 ms. A booster was given 2 weeks after the first immunization. The non-immunized mice were set up as controls (Chen et al., 2009).
• Challenge Protocol: Two weeks after the last immunization, the mice were anesthetized and challenged with 20 μl of the viral suspension containing 5LD50 influenza virus A/Chicken/Henan/12/2004(H5N1) or A/PR/8/34 (H1N1) by intranasal drip (Chen et al., 2009).
• Efficacy: Plasmids carrying the HA, NA, NP, M1, and M2 genes, respectively, of the avian influenza virus (AIV) A/Chicken/Henan/12/2004(H5N1) strain were administered to mice, and the mice were challenged with the homologous virus later. The mice immunized with M1 plasmid survived 25% with five immunizations. The mixture of M1 and NP plasmids protected 95% of the mice against the homologous virus, and 80% of the mice against a challenge with heterologous H1N1 (PR8) virus (Chen et al., 2009).