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Vaccine Detail

Cancer DNA Vaccine encoding Endoglin Boosted with Recombinant Endoglin Protein
Vaccine Information
  • Vaccine Name: Cancer DNA Vaccine encoding Endoglin Boosted with Recombinant Endoglin Protein
  • Target Pathogen: Cancer
  • Target Disease: Cancer
  • Vaccine Ontology ID: VO_0011367
  • Type: DNA vaccine
  • Status: Research
  • Eng gene engineering:
    • Type: DNA Vaccine Construction and Recombinant Prot
    • Description:
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pcDNA3.1 DNA vaccine plasmid
  • Immunization Route: i.m. injection for DNA, s.c. for protein
Host Response

Mouse Response

  • Host Strain: BALB/c, C57BL/6
  • Vaccination Protocol: The method of ppEDG DNA immunization; mice were injected i.m. in both quadriceps. A 1-ml insulin syringe was used for all injections and each single dose consisted of 100 μg that was diluted in normal saline of a total volume of 100 μl and split between both legs. The method of pEDG protein immunization; mice were injected s.c. and each single dose consisted of 10 μg that was also diluted in normal saline of a total volume of 100 μl. To investigate the protective anti-tumor effects mice at 6 to 8 weeks of age were randomly divided into the following 4 groups of 10 animals each. Group 1 (DP) mice were vaccinated with pEDG and ppEDG simultaneously once weekly for 4 continuous weeks. Group 2 (DD) mice were vaccinated with pEDG alone at the same time-points as in the group 1. Group 3 (PP) mice were vaccinated with ppEDG alone. Group 4 (NS) mice were injected with 100 μl normal saline. (Tan et al., 2007).
  • Immune Response: CTL response against endoglin-positive HUVECs, but not against endoglin-negative tumor cells was found in the mice combined DNA with protein vaccination. In addition, combination of endoglin DNA and recombinant protein vaccination significantly induced IFN-gamma secreting cells (Tan et al., 2007).
  • Challenge Protocol: One week after the last vaccination or saline injection all the experimental mice were subcutaneously injected with 2×106 live tumor cells (Tan et al., 2007).
  • Efficacy: The results showed that combination of endoglin DNA and protein vaccines could enhance both protective and therapeutic anti-tumor efficacy in both colon carcinoma and Lewis lung carcinoma models. Significant inhibition of tumor angiogenesis was found in the tumor tissues (Tan et al., 2007).
References
Tan et al., 2007: Tan GH, Li YN, Huang FY, Wang H, Bai RZ, Jang J. Combination of recombinant xenogeneic endoglin DNA and protein vaccination enhances anti-tumor effects. Immunological investigations. 2007; 36(4); 423-440. [PubMed: 17691024].