• Vaccine Name: rBCG-Ag85A[Tokyo]
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Vaccine Ontology ID: VO_0000565
• Type: Live, attenuated vaccine
• Antigen: BCG and Mycobacterium antigen Ag85A (Sugawara et al., 2007).
• FbpA (Ag85A) gene engineering:
  • Type: Recombinant vector construction
  • Description: The Ag85A gene was amplified by PCR and subcloned into the pCR4 vector. The gene was inserted into the pBBN vector (Ag85A-HA) possessing a hemagglutinin (HA) tag at its 5′ end. The Ag85A-HA was expressed in Escherichia coli. The Ag85A-HA gene was then introduced into the downstream region of the pHPS integration vector. The vector was then electroporated into BCG Tokyo. The resulting recombinant was named (rBCG-Ag85A[Tokyo]) (Sugawara et al., 2007).
  • Detailed Gene Information: Click Here.

Guinea pig Response

• Host Strain: Hartley female guinea pigs
• Vaccination Protocol: Hartley female guinea pigs (group 2) were vaccinated with BCG Tokyo (Sugawara et al., 2007).