• Vaccine Name: KLH-s-FimH1-25 with CFA and then IFA
• Target Pathogen: Escherichia coli
• Target Disease: Hemorrhagic colitis
• Type: Subunit vaccine
• Status: Research
• Host Species for Licensed Use: Mouse
• Antigen: key-hole limpet hemocyanin (KLH)-conjugated s-FimH1–25FimH peptide 1-25 aa (Thankavel et al., 1997)
• FimH from E. coli str. K-12 substr. MG1655 gene engineering:
  • Type: peptide 1-25 residues synthesis
  • Description:
  • Detailed Gene Information: Click Here.
• Immunization Route: intramuscularly and subcutaneously

Mouse Response

• Immune Response: Each mouse was injected intramuscularly and subcutaneously with 150 mg of KLH-conjugated synthetic peptide emulsified in CFA. 4 wk later, each animal was boosted with 150 mg of the same immunogen emulsified in incomplete Freund’s Adjuvant (Thankavel et al., 1997).
• Challenge Protocol: 5 d after intravesicular challenge with E. coli CI5 (50 ml of 1 3 109 CFU/ml PBS bacterial suspension), the bladder from each mouse was homogenized and the CFU determined (Thankavel et al., 1997).
• Efficacy: in vivo E. coli colonization in the bladders of mice actively immunized with synthetic FimH1–25 was significantly reduced (Thankavel et al., 1997).