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Vaccine Detail

M. tuberculosis DNA Vaccine encoding Ag85B Protein
Vaccine Information
  • Vaccine Name: M. tuberculosis DNA Vaccine encoding Ag85B Protein
  • Target Pathogen: Mycobacterium tuberculosis
  • Target Disease: Tuberculosis
  • Vaccine Ontology ID: VO_0011410
  • Type: DNA vaccine
  • Status: Research
  • Ag85B from M. tuberculosis H37Rv gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pRC DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: C57BL/6
  • Vaccination Protocol: Mice at or between 7 and 8 weeks old were immunized. Fifty micrograms of plasmid, both DNA-85B or control vector, was injected intramuscularly (i.m.) in 50 μl PBS into the hind leg. Mice were immunized two or four times at 2 week intervals. As a positive control, a single dose of BCG (105 cfu) was injected s.c. (Palma et al., 2007).
  • Challenge Protocol: Four weeks after the last boost, mice were challenged intravenously (i.v.) in a lateral tail vein with 105 cfu of MTB H37Rv (Palma et al., 2007).
  • Efficacy: A significant reduction of the bacterial load was observed in mice receiving two administrations of the DNA-85B vaccine (DNAx2-immunized mice) with respect to unvaccinated naïve control or empty-plasmid DNA recipient mice, indicating protection by the vaccine. The degree of reduction of the mycobacterial burden was not significantly different from that conferred by BCG, although the immunization with BCG caused the highest nominal reduction of mycobacteria in the lungs (Palma et al., 2007).
  • Information about this animal model: Mouse Model for TB research
References
Palma et al., 2007: Palma C, Iona E, Giannoni F, Pardini M, Brunori L, Orefici G, Fattorini L, Cassone A. The Ag85B protein of Mycobacterium tuberculosis may turn a protective immune response induced by Ag85B-DNA vaccine into a potent but non-protective Th1 immune response in mice. Cellular microbiology. 2007; 9(6); 1455-1465. [PubMed: 17250590].