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Vaccine Detail

P. falciparum MSP3 Protein Subunit Vaccine
Vaccine Information
  • Vaccine Name: P. falciparum MSP3 Protein Subunit Vaccine
  • Target Pathogen: Plasmodium spp.
  • Target Disease: Malaria
  • Vaccine Ontology ID: VO_0011440
  • Type: Subunit vaccine
  • Status: Research
  • msp3 gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: complete Freunds adjuvant
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Monkey Response

  • Host Strain: Aotus nancymai
  • Vaccination Protocol: Seven monkeys were vaccinated with 100 μg of EcMSP3, seven with 100 μg of control protein Pfs25, a parasite protein expressed during the mosquito stage of the life cycle. Each monkey received 0.125 mL of antigen emulsified in complete Freund's adjuvant at four sites, for a total of 0.5 mL, followed by two booster vaccinations with the same dose of antigen in a Montanide ISA51 (SEPPIC) formulation at 3-week intervals (Tsai et al., 2009).
  • Challenge Protocol: Seventeen days after the third vaccination, the monkeys were challenged by intravenous infusion of 5 × 104 P. falciparum FVO strain parasitized RBCs collected from a naïve donor monkey (Tsai et al., 2009).
  • Efficacy: By day 11 post-challenge, the parasitemia in all but one monkey in the control group had reached the predetermined upper limit and were treated In contrast, no animals in the EcMSP3-vaccinated group required treatment by this time (Tsai et al., 2009).
References
Tsai et al., 2009: Tsai CW, Duggan PF, Jin AJ, Macdonald NJ, Kotova S, Lebowitz J, Hurt DE, Shimp RL Jr, Lambert L, Miller LH, Long CA, Saul A, Narum DL. Characterization of a protective Escherichia coli-expressed Plasmodium falciparum merozoite surface protein 3 indicates a non-linear, multi-domain structure. Molecular and biochemical parasitology. 2009; 164(1); 45-56. [PubMed: 19073223].