VIOLIN: Vaccine Investigation and Online Information Network

Tir

General Information

Protegen ID

199

Sequence Strain (Species/Organism)

Escherichia coli O157:H7 str. EDL933

VO ID

VO_0010940

Taxonomy ID

155864

Molecule Role

Protective antigen

Molecule Role Annotation

Mice immunized intranasally with recombinant Tir protein had a survival rate of 92.9% after challenge with EHEC 0157:H7 (Fan et al., 2011).

Immunization of calves with recombinant EHEC O157 EspA, intimin and Tir resulted in the generation of antibodies capable of cross-reacting with antigens from non-O157 EHEC serotypes, suggesting that immunization with these antigens may provide a degree of cross-protection against other EHEC serotypes (McNeilly et al., 2015)

Related Vaccines(s)

E. coli O157:H7 intimin vaccine , E. coli O157:H7 subunit vaccine expressing Esps and Tir , Recombinant Tir Protein Vaccine

References

Fan et al., 2011: Fan HY, Wang L, Luo J, Long BG. Protection against Escherichia coli O157:H7 challenge by immunization of mice with purified Tir proteins. Molecular biology reports. 2011; ; . [PubMed: 21567195].

McNeilly et al., 2015: McNeilly TN, Mitchell MC, Corbishley A, Nath M, Simmonds H, McAteer SP, Mahajan A, Low JC, Smith DG, Huntley JF, Gally DL. Optimizing the Protection of Cattle against Escherichia coli O157:H7 Colonization through Immunization with Different Combinations of H7 Flagellin, Tir, Intimin-531 or EspA. PloS one. 2015; 10(5); e0128391. [PubMed: 26020530].

Gene Information

Gene Name

Tir

NCBI Gene ID

960860

Genbank Accession

AE005174

Locus Tag

Z5112

Gene Starting Position

4668912

Gene Ending Position

4670588

DNA Sequence

>NC_002655.2:4668912-4670588 Escherichia coli O157:H7 str. EDL933 genome
TTTAGACGAAACGATGGGATCCCGGCGCTGGTGGGTTATTCGAAGTATTCACAGCGCTATTACTCCCCCC
CGTTAATCCTCCCATGTCATGGCGTAATCCACCACTTAGCGCCAGACGCGCATAAGTGCTTTGAATCCCC
GCACTTGGATTTCCTAATAACCGTGCGCCGTTATCAGTAGTATCCCGGGGAGGATGTTGAATGGTGCTAT
ATACAACAGAATCTGTATTCCCCATATTCTGAACAGACGTATTAGAATTAGAAGTCGGCACCTGCGAATC
ATGCAGCGATGTTTTAACATCAGCATACGGATTCTGCACGGTCCCTATGCTGGAAGTGTCAAAGAAAGTC
GACGAGGTGCTAGCCATCGAGCTACGTCTGCTCTCCATGGTATCTTCTGACCCAGGGGTATCTACATTGC
CCTGTGCAGGTGTATTATTTGCAGGCTTATTCTCTACCGTACGTGCGCTTGTAGTTGTAGTTGTAGTAGT
TGTTGTTGTTGTTTGTTCTACCGGCTGATTTTTTCGATGAAGCGCAGCGGTGACGGCAACACCAATTCCC
CCACCAAGAATCAATGCGCCACTAAGACCGTAGCCAGCCCCCGATGAAACTTTCAGCTCCTCCTGGCGTT
TAGCTTGTTGTTCATCATATTTTTTTTGCGCCTGAGCATTATTTTCAATGGCTTGCTGTTTGGCCTCTTC
GCCTGCTGCTTTAGCCTGCTCTTCTATATTCGCAACAACATCATCTTTCAATACCCCTGACGGAATCGCA
TTTCCGAGCTCATCGATATTAACTTTTTGATTATCTGGGTTCTGGAACGCTTCTTTCGTTAACTGATCTC
TTGTCGCAGTTTCAGTTGCACTTGCAGCTGCATCAGGGTCGGTCGTGGTTGGGCTATCCGGCTCCGGCGT
CAATGCAAGCGCCTGTACAATACCCGTCGCCGCCAACCCTATCAGACCTGTAGCAACAGTCCCCAACGCC
AACCAAAGTTTAGGATCTGAACGAAGGCTGGAAGTTGAGGTTGAGGTCTGAGTTTCTGTGGTGTTTTCCG
CACCGCTATTTGACTCCCTCAACTCCCCAACGCCTTTTGACTCCCCAGCACCTTTGGACTCCCCGGTCCC
TTTGGGCTCTAACAGCTCCAGTATCCTTTGGCGGGCTTCCGTGATATCTGAAGCAACGGTGACCATAGCA
TGCCCAGCACCACCACGGCCTCCAGTAAATACAAATTTGTCTTTACCTTCAGGATCAATGGACTGCAAGC
GAGCGTACTCTTGATCACTTAAAACAACAGAGGTCTCAACACCATTCCTCTGACCGACAGCAATATGTTT
ACCATCTTCCTGAGTTTCAACTCGAAATACCGAAGAGCCAATCTGCCTGTTAAGAGTATCGAGCGGACCA
TGATCATGAAGAACTTCAAATCCATCATTCAGTGTTATCTCAGACGCCGCCAGGCGCATCGGATTTACAG
GAAGTCCAGGAACATCACTGGCACGATTGTCGCCAGAATCAGCCATAGAATTCCTTACAGGCGTAAATAG
CGCACGAGATCCCAACGGCCCCGTAGAGTTAATGAGCTGACCACGCCCCCCTGCACCGTCGGTTTGTGAA
GGTAATGGAGGTGCAGGAGGAATTGAATTATTCACATTGGGATTATGACCAAGATTACCAATAGGCA

Protein Information

Protein Name

translocated intimin receptor protein

NCBI Protein GI

15804222

Protein Accession

NP_290261

Protein pI

4.78

Protein Weight

54549.01

Protein Length

558

Protein Sequence

>NP_290261.1 translocated intimin receptor protein [Escherichia coli O157:H7 str. EDL933]
MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGSRALFTPVRNSMADSGDNRASDVPGLP
VNPMRLAASEITLNDGFEVLHDHGPLDTLNRQIGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYAR
LQSIDPEGKDKFVFTGGRGGAGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGA
ENTTETQTSTSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATETATR
DQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAIENNAQAQKKYDEQQAK
RQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQTTTTTTTTTTTSARTVENKPANNTPAQG
NVDTPGSEDTMESRRSSMASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVY
STIQHPPRDTTDNGARLLGNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV

Epitope Information

IEDB Linear Epitope