VIOLIN: Vaccine Investigation and Online Information Network

G glycoprotein

General Information

Protegen ID

513

Sequence Strain (Species/Organism)

Infectious hematopoietic necrosis virus

VO ID

VO_0011109

Taxonomy ID

11290

Molecule Role

Protective antigen

Molecule Role Annotation

The DNA vaccine pIHNw-G encodes the glycoprotein of the fish rhabdovirus infectious hematopoietic necrosis virus (IHNV). Vaccine performance in rainbow trout was measured 3, 6, 13, 24, and 25 months after vaccination. At three months all fish vaccinated with 0.1 microg pIHNw-G had detectable neutralizing antibody (NAb) and they were completely protected from lethal IHNV challenge with a relative percent survival (RPS) of 100% compared to control fish (Kurath et al., 2006).

Related Vaccines(s)

IHNV DNA vaccine pCDNA-G encoding G , IHNV DNA vaccine pCMV4-G , IHNV DNA vaccine pIHN-G encoding glycoproteins , IHNV DNA vaccine pIHNw-G

References

Kurath et al., 2006: Kurath G, Garver KA, Corbeil S, Elliott DG, Anderson ED, LaPatra SE. Protective immunity and lack of histopathological damage two years after DNA vaccination against infectious hematopoietic necrosis virus in trout. Vaccine. 2006; 24(3); 345-354. [PubMed: 16154239].

Gene Information

Gene Name

G glycoprotein

NCBI Gene ID

1489848

Genbank Accession

KP216199

Locus Tag

IHNVgp4

Gene Starting Position

2998

Gene Ending Position

4524

DNA Sequence

>NC_001652.1:2998-4524 Infectious hematopoietic necrosis virus, complete genome
AATGGACACCATGATCACCACTCCGCTCATTCTCATTCTGATCACCTGCGGAGCAAACAGCCAAACCGTC
AAACCCGACACCGCAAGCGAATCAGACCAACCCACCTGGTCAAACCCGCTCTTCACCTATCCCGAGGGAT
GCACTCTGGACAAGCTCTCTAAGGTCAATGCCTCTCAACTGAGATGCCCAAGGATCTTCGACGATGAGAA
CAGGGGGCTAATTGCCTATCCCACATCCATCCGGTCCCTGTCAGTCGGAAACGACCTCGGGGACATTCAC
ACCCAAGGGAACCACATCCACAAAGTCCTGTACCGCACAATCTGCTCAACAGGGTTCTTCGGGGGTCAGA
CGATAGAGAAGGCGCTTGTAAAAATGAAACTCTCTACGAAAGAAGCAGGGGCGTATGACACCACAACCGC
AGCCGCTCTGTACTTCCCAGCTCCCCGATGCCAATGGTACACCGACAACGTACAAAACGATCTCATCTTC
TACTACACAACACAAAAGAGTGTTCTTAGAGATCCCTACACCAGAGACTTTCTGGACTCAGATTTTATTG
GAGGAAAATGTACCAAATCACCCTGCCAGACTCATTGGTCCAACGTAGTTTGGATGGGTGATGCAGGGAT
ACCAGCCTGTGATTCCAGCCAAGAGATAAAAGCTCACCTCTTTGTTGATAAAATCTCCAATCGAGTCGTG
AAGGCAACGAGCTACGGACACCACCCCTGGGGACTGCATCAGGCCTGTATGATTGAATTCTGTGGGCAAC
AGTGGATACGGACAGATCTCGGTGACCTAATATCTGTCGTATACAATTCTGGATCAGAAATCCTCTCGTT
CCCGAAGTGTGAAGACAAGACCGTGGGAATGAGGGGAAACTTGGATGACTTTGCCTATCTAGACGATCTG
GTGAAGGCCTCTGAGAGCAGAGAGGAATGTCTTGAGGCGCACGCCGAGATAATATCAACAAACAGTGTGA
CTCCATACCTCCTATCCAAATTCCGATCTCCACATCCCGGAATAAATGACGTCTACGCTATGCACAAAGG
CTCCATCTATCACGGGATGTGCATGACGGTCGCTGTGGACGAGGTATCCAAGGACAGGACAACGTACAGG
GCCCATCGCGCTACCAGCTTCACGAAATGGGAACGACCCTTTGGGGATGAGTGGGAGGGCTTTCACGGAT
TGCACGGAAACAACACCACCATTATTCCAGACCTGGAGAAATACGTCGCCCAGTACAAGATGAGCATGAT
GGAACCGATGAGCATCAAATCCGTACCCCATCCAAGCATCCTGGCCCTCTACAATGAGACAGACGTGTCA
GGGATCTCCATCAGGAAACTGGACTCATTCGACCTTCAATCACTCCACTGGAGTTTCTGGCCCACAATCT
CCGCACTGGGTGGAATTCCCTTTGTTCTCCTCCTTGCTGTTGCCGCGTGCTGCTGCTGGTCAGGGAGACC
TCCCACTCCCTCCGTGCCGCAGAGTATCCCCATGTATCACCTGGCAAACCGGTCCTA

Protein Information

Protein Name

glycoprotein

NCBI Protein GI

9628086

Protein Accession

NP_042679

Protein pI

6.5

Protein Weight

54073.98

Protein Length

508

Protein Sequence

>NP_042679.1 glycoprotein [Infectious hematopoietic necrosis virus]
MDTMITTPLILILITCGANSQTVKPDTASESDQPTWSNPLFTYPEGCTLDKLSKVNASQLRCPRIFDDEN
RGLIAYPTSIRSLSVGNDLGDIHTQGNHIHKVLYRTICSTGFFGGQTIEKALVKMKLSTKEAGAYDTTTA
AALYFPAPRCQWYTDNVQNDLIFYYTTQKSVLRDPYTRDFLDSDFIGGKCTKSPCQTHWSNVVWMGDAGI
PACDSSQEIKAHLFVDKISNRVVKATSYGHHPWGLHQACMIEFCGQQWIRTDLGDLISVVYNSGSEILSF
PKCEDKTVGMRGNLDDFAYLDDLVKASESREECLEAHAEIISTNSVTPYLLSKFRSPHPGINDVYAMHKG
SIYHGMCMTVAVDEVSKDRTTYRAHRATSFTKWERPFGDEWEGFHGLHGNNTTIIPDLEKYVAQYKMSMM
EPMSIKSVPHPSILALYNETDVSGISIRKLDSFDLQSLHWSFWPTISALGGIPFVLLLAVAACCCWSGRP
PTPSVPQSIPMYHLANRS

Vaxign Prediction

Localization(Probability)

(Prob.=0)

Adhesin Probability

0.526

Trans-membrane Helices

Detailed Vaxign Results

Vaxign Results

Epitope Information

IEDB Linear Epitope