VIOLIN: Vaccine Investigation and Online Information Network

PspA

General Information

Protegen ID

620

Sequence Strain (Species/Organism)

Streptococcus pneumoniae D39

VO ID

VO_0011204

Taxonomy ID

373153

Molecule Role

Protective antigen

Molecule Role Annotation

Groups of BALB/c mice immunized with choline binding protein PspA were challenged with either D39 or WCH16. PspA elicited protection by using moderate challenge doses of S. pneumoniae (Ogunniyi et al., 2007).

COG

COG5263R, under R: General function prediction only

Related Vaccines(s)

DNA vaccine expressing PspA , S. pneumoniae PspA Protein Vaccine , S. pneumoniae pspA-rBCG Vector Vaccine

References

Ogunniyi et al., 2007: Ogunniyi AD, Grabowicz M, Briles DE, Cook J, Paton JC. Development of a vaccine against invasive pneumococcal disease based on combinations of virulence proteins of Streptococcus pneumoniae. Infection and immunity. 2007; 75(1); 350-357. [PubMed: 17088353].

Gene Information

Gene Name

PspA

NCBI Gene ID

4441373

Genbank Accession

CP000410

Locus Tag

SPD_0126

Gene Starting Position

128355

Gene Ending Position

130214

DNA Sequence

>NC_008533.1:128355-130214 Streptococcus pneumoniae D39, complete genome
GATGAATAAGAAAAAAATGATTTTAACAAGTCTAGCCAGCGTCGCTATCTTAGGGGCTGGTTTTGTTGCG
TCTCAGCCTACTGTTGTAAGAGCAGAAGAATCTCCCGTAGCCAGTCAGTCTAAAGCTGAGAAAGACTATG
ATGCAGCGAAGAAAGATGCTAAGAATGCGAAAAAAGCAGTAGAAGATGCTCAAAAGGCTTTAGATGATGC
AAAAGCTGCTCAGAAAAAATATGACGAGGATCAGAAGAAAACTGAGGAGAAAGCCGCGCTAGAAAAAGCA
GCGTCTGAAGAGATGGATAAGGCAGTGGCAGCAGTTCAACAAGCGTATCTAGCCTATCAACAAGCTACAG
ACAAAGCCGCAAAAGACGCAGCAGATAAGATGATAGATGAAGCTAAGAAACGCGAAGAAGAGGCAAAAAC
TAAATTTAATACTGTTCGAGCAATGGTAGTTCCTGAGCCAGAGCAGTTGGCTGAGACTAAGAAAAAATCA
GAAGAAGCTAAACAAAAAGCACCAGAACTTACTAAAAAACTAGAAGAAGCTAAAGCAAAATTAGAAGAGG
CTGAGAAAAAAGCTACTGAAGCCAAACAAAAAGTGGATGCTGAAGAAGTCGCTCCTCAAGCTAAAATCGC
TGAATTGGAAAATCAAGTTCATAGACTAGAACAAGAGCTCAAAGAGATTGATGAGTCTGAATCAGAAGAT
TATGCTAAAGAAGGTTTCCGTGCTCCTCTTCAATCTAAATTGGATGCCAAAAAAGCTAAACTATCAAAAC
TTGAAGAGTTAAGTGATAAGATTGATGAGTTAGACGCTGAAATTGCAAAACTTGAAGATCAACTTAAAGC
TGCTGAAGAAAACAATAATGTAGAAGACTACTTTAAAGAAGGTTTAGAGAAAACTATTGCTGCTAAAAAA
GCTGAATTAGAAAAAACTGAAGCTGACCTTAAGAAAGCAGTTAATGAGCCAGAAAAACCAGCTCCAGCTC
CAGAAACTCCAGCCCCAGAAGCACCAGCTGAACAACCAAAACCAGCGCCGGCTCCTCAACCAGCTCCCGC
ACCAAAACCAGAGAAGCCAGCTGAACAACCAAAACCAGAAAAAACAGATGATCAACAAGCTGAAGAAGAC
TATGCTCGTAGATCAGAAGAAGAATATAATCGCTTGACTCAACAGCAACCGCCAAAAGCTGAAAAACCAG
CTCCTGCACCAAAAACAGGCTGGAAACAAGAAAACGGTATGTGGTACTTCTACAATACTGATGGTTCAAT
GGCGACAGGATGGCTCCAAAACAACGGTTCATGGTACTACCTCAACAGCAATGGTGCTATGGCTACAGGT
TGGCTCCAATACAATGGTTCATGGTATTACCTCAACGCTAACGGCGCTATGGCAACAGGTTGGGCTAAAG
TCAACGGTTCATGGTACTACCTCAACGCTAATGGTGCTATGGCTACAGGTTGGCTCCAATACAACGGTTC
ATGGTATTACCTCAACGCTAACGGCGCTATGGCAACAGGTTGGGCTAAAGTCAACGGTTCATGGTACTAC
CTCAACGCTAATGGTGCTATGGCTACAGGTTGGCTCCAATACAACGGTTCATGGTACTACCTCAACGCTA
ACGGTGCTATGGCTACAGGTTGGGCTAAAGTCAACGGTTCATGGTACTACCTCAACGCTAATGGTGCTAT
GGCAACAGGTTGGGTGAAAGATGGAGATACCTGGTACTATCTTGAAGCATCAGGTGCTATGAAAGCAAGC
CAATGGTTCAAAGTATCAGATAAATGGTACTATGTCAATGGTTTAGGTGCCCTTGCAGTCAACACAACTG
TAGATGGCTATAAAGTCAATGCCAATGGTGAATGGGTTTA

Protein Information

Protein Name

surface protein A

NCBI Protein GI

116515876

Protein Accession

YP_815641

Protein pI

4.82

Protein Weight

66023.6

Protein Length

619

Protein Note

equivalent gene in S.pneumoniae R6 = spr0121; identified by match to protein family HMM PF01473

Protein Sequence

>YP_815641.1 surface protein A [Streptococcus pneumoniae D39]
MNKKKMILTSLASVAILGAGFVASQPTVVRAEESPVASQSKAEKDYDAAKKDAKNAKKAVEDAQKALDDA
KAAQKKYDEDQKKTEEKAALEKAASEEMDKAVAAVQQAYLAYQQATDKAAKDAADKMIDEAKKREEEAKT
KFNTVRAMVVPEPEQLAETKKKSEEAKQKAPELTKKLEEAKAKLEEAEKKATEAKQKVDAEEVAPQAKIA
ELENQVHRLEQELKEIDESESEDYAKEGFRAPLQSKLDAKKAKLSKLEELSDKIDELDAEIAKLEDQLKA
AEENNNVEDYFKEGLEKTIAAKKAELEKTEADLKKAVNEPEKPAPAPETPAPEAPAEQPKPAPAPQPAPA
PKPEKPAEQPKPEKTDDQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPAPKTGWKQENGMWYFYNTDGSM
ATGWLQNNGSWYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYNGS
WYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAM
ATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV

Epitope Information

IEDB Linear Epitope