VIOLIN: Vaccine Investigation and Online Information Network

MSP1 from P. yoelii str. 17XNL

General Information

Protegen ID

628

Sequence Strain (Species/Organism)

Plasmodium yoelii

VO ID

VO_0011212

Taxonomy ID

5861

Molecule Role

Protective antigen

Molecule Role Annotation

Researchers found that the cysteine-rich, carboxyl-terminal region of the MSP-1 protein from the rodent malarial parasite Plasmodium yoelii yoelii can be expressed in a native configuration as a fusion protein in Escherichia coli. This recombinant polypeptide elicits antibodies in mice which recognize the native parasite MSP-1. Most significantly, both inbred and outbred mice immunized with the fusion protein in Ribi adjuvant are partially and in some cases completely protected against challenge infection with an otherwise lethal parasite strain (Daly and Long, 1993).

Related Vaccines(s)

P. yoelii DNA vaccine encoding MSP1 , rBCGMSP1-15

References

Daly and Long, 1993: Daly TM, Long CA. A recombinant 15-kilodalton carboxyl-terminal fragment of Plasmodium yoelii yoelii 17XL merozoite surface protein 1 induces a protective immune response in mice. Infection and immunity. 1993; 61(6); 2462-2467. [PubMed: 8363656].

Gene Information

Gene Name

MSP1 from P. yoelii str. 17XNL

NCBI Gene ID

3791597

Genbank Accession

LK934636

Chromosome No

Locus Tag

PY17X_0834400

Gene Starting Position

1276003

Gene Ending Position

1281321

DNA Sequence

>NC_036180.1:1276003-1281321 Plasmodium yoelii genome assembly PY17X01, chromosome : 8
AATGAAGGTGATTGGACTTTTATTTTCTTTCGTTTTTTTTGCTATAAAATGCAAATCTGAAACAATTGAA
GTTTATAATGATCTCATTCAAAAGTTAGAAAAATTAGAATCATTGTCAGTGGATGGGTTAGAACTATTTC
AAAAAAGTCAAGTAATTATAAATGCAACACAACCAACTGAAACTATTGATCCATTTACAAATCATAACTT
TGCACAACAAGTACAAGATTTTGTTACAAAATTTGAAGGATTAGGATTTACAGAACAAACAGAATTAGTC
AATTTAATAAAAGCATTAACCCCAAATAGATATGGAGTAAAATATTTAATTGAAAGTAAAGAAGAATTTA
ATGGATTAATGCACGCAATAAATTTTTATTATGATGTACTTAGAGATAAATTAAATGATATGTGTGCAAA
TAATTATTGTGAAATTCCTGAACATCTTAAAATTAGTGAAGAAGAAACAGAAATGCTTAAAAAAGTAATT
TTAGGTTATAGAAAACCAATAGAAAATATTCAAGACGATATTGAAAAGTTAGAAATTTACATAGAAAGAA
ATAAAGAAACTGTTGCAGCTTTAAACGCTCTTATTGCTGAAGAAACAAAAAAAATACAACCTGAAGGTAA
CGAAGATTGCAATGACGCTAGTTGTGATAGCGATAAATATAATAAAAAAAAACCAATATACCAAGCTATG
TACAATGTTATATTTTACAAAAAACAATTAGCTGAAATACAAAAGGTTGTCGAAGTCTTAGAAAAACGAG
TTTCTACATTAAAGAAAAATGATGCCATCAAACCATTATGGCAACAAATTGAAGTTCTCAATGCTGCCCC
CGTCGTCACTGCCGAAACACAAATAGTTACAGGAGGACAATCTAGTACAGAACCAGGTAGTGGTGGATCA
AGTGCATCGGGAACAAGTTCATCAGGACAAGCTAGTGCAGGAACAGGTGTAGAACAAGCTAACACTGTAG
CATCTGTTACAGTAACACCTAGTGTAGGACAAAATGGTGAAGCATCAACTAATCCACAAACAGCTCAAGT
GCAACCCGTTCCAACTCTTACATTAGAAGAAAAACAGAAAAAAATAGCCGGACTTTATGCTCAAATTAAA
GAAATTGCAAAAACTATAAAATTCAACTTAGAAGGAATATTTGTAGATCCAATCGAATTAGAATATTTCA
AAAAAGAAAAAAAAAAAGAAAGTTGCAATTTATCAACTTCATCCTGTAAAAAAAATAAAGCATCCGAAAC
TATAATACCATTAACTATACGTTATCCAAATGGTATTAGTTACCCATTACCTGAAAATGATGTTTACAAT
AAAATTGCCAATAATGCCGCTGAAACAACATATGGTGATTTGACACATCCCGATAATACACCATTAACAG
GAGATTTAGCCACAAATGAACAAGCCAGAAAAGATCTAATAAAAGCTATTAAAAAGAAAATAAAAGCAGA
AGAAAAAAAATTAGAAACATTAAAAACGAATTATGATAATAAACTTACAGAATTTAATCAACAAAAAACT
CCATTCAAAGAAGCAGCTAAAGAATTTTATGAATCAAAATTTAGAAATAAATTGACTTCTGAAATTTTTG
AAAAATTCAAAACAAAAAGAGATGAATATATGACCAAGAAAACCGAATTAAACACTTGTGAATATGGAAA
TACTAAAGAATTAATTAATAAATTAAATAAACAACTTAATTATTTACAAGATTATTCATTAAGAAAAGAT
ATAATTAGTAATGAAATTGAATATTTTTCAAATAAAAAAAAAGAATTACAATATAATATTAATAGATTAG
CAGAAGCTGTTCAAGCAAAACAAAATGTATTAGTTGCATCAAAAGATGTACCACTTTCAACACTTGTAGA
ATTGCAAATACAAAAATCTTTATTAACAAAACAAATTGAGCAATTAAATAAAACTGAAGTATCTTTAAAC
AAAGCTCAATTAAAAGACAAACTATATGTTCCAAAAACATACGGTAATGAAGGAAAACCAGAACCATACT
ATTTAATAGCTGTAAAAAAAGAAGTTGACAGACTTGCCCAATTTATTCCAAAAATCGAAAGTATGATTGC
TAAAGAGAAGGAAAGAATGGAACAAGGACCTGCAATTACTGGAGAATCTGAAGAAGTACCATCTGGCCCT
AGTGCTGAATCATCAACAGATAGATCAACACAATCTTCAACATCCTCATCCTCATCCTCATCTTCAACCC
CAGCAGCAGCAGAATCCTCCTCAGCCACATTACCAGAAGCACCCGCACCAGCAGAAGCAGCATCCCCATC
AACAGAAGCATCAGAAGAAACAACAATACCCCCTACCACACAAGAAACACAACCATCACAAGCTGCATCA
TCCACAACACCTGCAAAACCAGTTATGACAAAATTATATTATCTTGAAAAATTACAAAAATTTTTAGTAT
TCTCATATTCATGCCATAAATACGTTTTACTACAAAACTCTACCATAAACAAAGATGCTTTAAGCAAATA
TGCTCTTACATCTGAAGAAGATAAAATAAGAACATTAAAAAGATGCAGTGAATTAGATGTATTATTAGCT
ATTCAAAATAATATGCCTACTATGTATTCACTTTATGAAAGTATAGTTGATGGTTTACAAAACATTTATA
CTGAATTATATGAAAAAGAAATGATGTATCATATATATAAATTAAAAGATGAAAACCCATCTATTAAATC
TTTATTGGTAAAAGCTGGCGTCATTGAACCAGAACCAGTAGCAGCACCAACACCAGTAACTCCAGCAGCA
ACAGAACAACAACAACAACAAGCAACACCTGATGTACAATCAGATGCACCAGCACCATCAGATGTCTCGC
AACAACCAGAAACACCAGTAACATCCACGACACCAGAGGTAACAACCTCAACAGAAGCATCATCATCAGC
ACCTGGCGAAGGTACACCATCAGGAGAAGCAGGAGCATCAGGAACAGAAGGAGCAACAGCATCTAACGCA
GCCACACCAGCAGGAACATCAGCATCAGGATCAGCAGCATCTAACGCAAGTACAACCTCAGATGTAACAC
CCCCAGCAGCAGCGGCAGCAGTACCATCAACATCTACACCAGCACCTGCACAACCACCAGCAGCAAATTC
TCAATCAGGAAACCCTGACTCAGGTATTAGATCACGAGCAGAAAGTGAAGAGGATATGCCTGCCGATGAT
TTTGAATTAGACAATTTATACAAATCTTACTTACAACAAATTGATGGAAATAATACTGAATTCATAAATT
TTATAAAATCTAAAAAAGAATTAATAAAAGCATTGACACCTGAAAAAGTTAATCAATTATATCTTGAAAT
CGCTCACTTAAAGGAATTATCAGAACATTATTATGATCGTTATTCTACATATAAATTAAAATTAGAAAGA
TTATATAACAAACATGAACAAATTCAACTAACCAATCGACAAATTAGAGATCTTAGTATATTGAAAGCAC
GATTATTAAAAAGAAAACAAACTCTTAATGGCGTATTTTATATATTAAATGGTTATGTAAATTTCTTTAA
CAAGAGAAGAGAAGCTGAAAAACAATATGTAGATAATGCATTAAAAAATACTGATATGTTATTAAAATAC
TACAAAGCTCGTACTAAATATTTTACTTCTGAAGCTGTTCCTTTAAAAACATTATCTAAAGCATCACTTG
ACAGAGAATCCAATTATTTGAAAATCGAAAAATTCAGAGCATACAGTCGATTAGAATTAAGATTAAAAAA
AAATATTAATTTAGGAAAGGAAAGAATTTCATATGTATCAGGAGGTTTACACCACGTATTTGAAGAATTT
AAAGAACTTATAAAAGATAAAGACTATACCGGAAAAAAAAACCCTGATAATGCCCCTGAAGTTACCAATG
CATTCGAACAATATAAAGAATTGCTTCCAAAGGGAGTAACAGTTTCAACTCCAGCAGTCGCAGTTACAAC
GACACTAGCAGCTGACGCACCAGCAACACCAGAAGGAGCAGTACCAGGAGCAGTACCAGGAGCTGTACCA
GGTGCAGTACCAGGAGCAGTACCAGGTGCAGTACCAGGATCAGGAACCGATACACGGGTAGCTGGAAGCA
GTGTTGATGATAATGAAGACGATGATATATATCAAATTGCAAGTGGTCAATCCGAAGATGCACCAGAAAA
AGATATTCTTTCCGAATTTACAAATGAAAGTTTGTATGTATACACAAAAAGGTTGGGTAGTACATATAAA
TCATTAAAGAAACACATGTTAAGAGAATTTTCAACAATTAAAGAAGACATGACAAATGGATTAAATAATA
AATCACAAAAAAGAAATGATTTCCTTGAAGTATTAAGCCATGAATTAGATTTATTCAAAGATTTAAGTAC
CAACAAATATGTTATTAGAAATCCATATCAATTATTAGATAATGATAAAAAAGACAAACAAATAGTAAAC
TTAAAATATGCTACTAAAGGTATAAATGAAGATATAGAAACAACTACTGACGGAATTAAATTCTTTAACA
AAATGGTTGAATTATACAACACTCAATTAGCTGCAGTAAAGGAACAAATTGCTACCATAGAAGCTGAAAC
TAACGATACCAATAAAGAAGAAAAAAAGAAATATATTCCAATCCTTGAAGATCTTAAAGGATTATATGAA
ACCGTAATAGGTCAAGCAGAAGAATATTCAGAAGAATTACAAAATAGACTTGATAATTATAAAAATGAAA
AAGCTGAATTTGAAATATTAACAAAAAATTTAGAAAAATACATACAAATTGACGAAAAACTTGACGAATT
TGTAGAACATGCAGAAAATAATAAACACATAGCCTCAATAGCTTTAAACAACTTAAATAAATCTGGTTTA
GTAGGAGAAGGTGAATCAAAGAAAATATTAGCAAAAATGCTTAACATGGATGGTATGGATTTATTAGGTG
TAGACCCTAAACATGTATGTGTTGATACAAGAGATATTCCTAAAAATGCTGGATGTTTTAGAGATGATAA
TGGTACTGAAGAATGGAGATGTTTATTAGGTTACAAAAAAGGTGAAGGTAATACATGTGTAGAAAATAAT
AATCCTACTTGTGATATCAACAATGGTGGATGTGATCCAACTGCTAGTTGTCAAAATGCGGAAAGTACGG
AAAATTCCAAAAAAATTATATGTACATGTAAAGAACCAACCCCTAATGCATATTATGAAGGTGTATTCTG
TAGTTCTTCCAGCTTTATGGGATTATCAATTTTATTAATTATCACATTAATTGTATTTAATATATTTTA

Protein Information

NCBI Protein GI

82596427

Protein Accession

XP_726257

Protein pI

5.05

Protein Weight

188296.05

Protein Length

1772

Protein Sequence

>XP_726257.1 merozoite surface protein 1 [Plasmodium yoelii]
MKVIGLLFSFVFFAIKCKSETIEVYNDLIQKLEKLESLSVDGLELFQKSQVIINATQPTETIDPFTNHNF
AQQVQDFVTKFEGLGFTEQTELVNLIKALTPNRYGVKYLIESKEEFNGLMHAINFYYDVLRDKLNDMCAN
NYCEIPEHLKISEEETEMLKKVILGYRKPIENIQDDIEKLEIYIERNKETVAALNALIAEETKKIQPEGN
EDCNDASCDSDKYNKKKPIYQAMYNVIFYKKQLAEIQKVVEVLEKRVSTLKKNDAIKPLWQQIEVLNAAP
VVTAETQIVTGGQSSTEPGSGGSSASGTSSSGQASAGTGVEQANTVASVTVTPSVGQNGEASTNPQTAQV
QPVPTLTLEEKQKKIAGLYAQIKEIAKTIKFNLEGIFVDPIELEYFKKEKKKESCNLSTSSCKKNKASET
IIPLTIRYPNGISYPLPENDVYNKIANNAAETTYGDLTHPDNTPLTGDLATNEQARKDLIKAIKKKIKAE
EKKLETLKTNYDNKLTEFNQQKTPFKEAAKEFYESKFRNKLTSEIFEKFKTKRDEYMTKKTELNTCEYGN
TKELINKLNKQLNYLQDYSLRKDIISNEIEYFSNKKKELQYNINRLAEAVQAKQNVLVASKDVPLSTLVE
LQIQKSLLTKQIEQLNKTEVSLNKAQLKDKLYVPKTYGNEGKPEPYYLIAVKKEVDRLAQFIPKIESMIA
KEKERMEQGPAITGESEEVPSGPSAESSTDRSTQSSTSSSSSSSSTPAAAESSSATLPEAPAPAEAASPS
TEASEETTIPPTTQETQPSQAASSTTPAKPVMTKLYYLEKLQKFLVFSYSCHKYVLLQNSTINKDALSKY
ALTSEEDKIRTLKRCSELDVLLAIQNNMPTMYSLYESIVDGLQNIYTELYEKEMMYHIYKLKDENPSIKS
LLVKAGVIEPEPVAAPTPVTPAATEQQQQQATPDVQSDAPAPSDVSQQPETPVTSTTPEVTTSTEASSSA
PGEGTPSGEAGASGTEGATASNAATPAGTSASGSAASNASTTSDVTPPAAAAAVPSTSTPAPAQPPAANS
QSGNPDSGIRSRAESEEDMPADDFELDNLYKSYLQQIDGNNTEFINFIKSKKELIKALTPEKVNQLYLEI
AHLKELSEHYYDRYSTYKLKLERLYNKHEQIQLTNRQIRDLSILKARLLKRKQTLNGVFYILNGYVNFFN
KRREAEKQYVDNALKNTDMLLKYYKARTKYFTSEAVPLKTLSKASLDRESNYLKIEKFRAYSRLELRLKK
NINLGKERISYVSGGLHHVFEEFKELIKDKDYTGKKNPDNAPEVTNAFEQYKELLPKGVTVSTPAVAVTT
TLAADAPATPEGAVPGAVPGAVPGAVPGAVPGAVPGSGTDTRVAGSSVDDNEDDDIYQIASGQSEDAPEK
DILSEFTNESLYVYTKRLGSTYKSLKKHMLREFSTIKEDMTNGLNNKSQKRNDFLEVLSHELDLFKDLST
NKYVIRNPYQLLDNDKKDKQIVNLKYATKGINEDIETTTDGIKFFNKMVELYNTQLAAVKEQIATIEAET
NDTNKEEKKKYIPILEDLKGLYETVIGQAEEYSEELQNRLDNYKNEKAEFEILTKNLEKYIQIDEKLDEF
VEHAENNKHIASIALNNLNKSGLVGEGESKKILAKMLNMDGMDLLGVDPKHVCVDTRDIPKNAGCFRDDN
GTEEWRCLLGYKKGEGNTCVENNNPTCDINNGGCDPTASCQNAESTENSKKIICTCKEPTPNAYYEGVFC
SSSSFMGLSILLIITLIVFNIF

Epitope Information

IEDB Linear Epitope