VIOLIN: Vaccine Investigation and Online Information Network

ClfA

General Information

Protegen ID

847

Sequence Strain (Species/Organism)

Staphylococcus aureus subsp. aureus str. Newman

VO ID

VO_0012390

Taxonomy ID

426430

Molecule Role

Protective antigen

Molecule Role Annotation

Mice immunized with recombinant ClfA and challenged with S. aureus developed less-severe arthritis than did mice immunized with a control antigen (Josefsson et al., 2001).
full-length ClfA N123 is required for maximal protection both locally and systemically.(Lacey et al., 2017)

Related Vaccines(s)

S. aureus ClfA Protein Vaccine , S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna , S. aureus DNA vaccine pClfaSrtD13

References

Josefsson et al., 2001: Josefsson E, Hartford O, O'Brien L, Patti JM, Foster T. Protection against experimental Staphylococcus aureus arthritis by vaccination with clumping factor A, a novel virulence determinant. The Journal of infectious diseases. 2001; 184(12); 1572-1580. [PubMed: 11740733].

Lacey et al., 2017: Lacey KA, Leech JM, Lalor SJ, McCormack N, Geoghegan JA, McLoughlin RM. The Staphylococcus aureus Cell Wall-Anchored Protein Clumping Factor A Is an Important T Cell Antigen. Infection and immunity. 2017; 85(12); . [PubMed: 28947645].

Gene Information

Gene Name

ClfA

NCBI Gene ID

5330438

Genbank Accession

AP009351

Locus Tag

NWMN_0756

Gene Starting Position

849756

Gene Ending Position

852557

DNA Sequence

>NC_009641.1:849756-852557 Staphylococcus aureus subsp. aureus str. Newman DNA, complete genome
AATGAATATGAAGAAAAAAGAAAAACACGCAATTCGGAAAAAATCGATTGGCGTGGCTTCAGTGCTTGTA
GGTACGTTAATCGGTTTTGGACTACTCAGCAGTAAAGAAGCAGATGCAAGTGAAAATAGTGTTACGCAAT
CTGATAGCGCAAGTAACGAAAGCAAAAGTAATGATTCAAGTAGCGTTAGTGCTGCACCTAAAACAGACGA
CACAAACGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCA
GCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAG
CTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGA
ATTAGTGAATCAAACAAGTAATGAAACGACTTTTAATGATACTAATACAGTATCATCTGTAAATTCACCT
CAAAATTCTACAAATGCGGAAAATGTTTCAACAACGCAAGATACTTCAACTGAAGCAACACCTTCAAACA
ATGAATCAGCTCCACAGAGTACAGATGCAAGTAATAAAGATGTAGTTAATCAAGCGGTTAATACAAGTGC
GCCTAGAATGAGAGCATTTAGTTTAGCGGCAGTAGCTGCAGATGCACCGGCAGCTGGCACAGATATTACG
AATCAGTTGACGAATGTGACAGTTGGTATTGACTCTGGTACGACTGTGTATCCGCACCAAGCAGGTTATG
TCAAACTGAATTATGGTTTTTCAGTGCCTAATTCTGCTGTTAAAGGTGACACATTCAAAATAACTGTACC
TAAAGAATTAAACTTAAATGGTGTAACTTCAACTGCTAAAGTGCCACCAATTATGGCTGGAGATCAAGTA
TTGGCAAATGGTGTAATCGATAGTGATGGTAATGTTATTTATACATTTACAGACTATGTAAATACTAAAG
ATGATGTAAAAGCAACTTTGACCATGCCCGCTTATATTGACCCTGAAAATGTTAAAAAGACAGGTAATGT
GACATTGGCTACTGGCATAGGTAGTACAACAGCAAACAAAACAGTATTAGTAGATTATGAAAAATATGGT
AAGTTTTATAACTTATCTATTAAAGGTACAATTGACCAAATCGATAAAACAAATAATACGTATCGTCAGA
CAATTTATGTCAATCCAAGTGGAGATAACGTTATTGCGCCGGTTTTAACAGGTAATTTAAAACCAAATAC
GGATAGTAATGCATTAATAGATCAGCAAAATACAAGTATTAAAGTATATAAAGTAGATAATGCAGCTGAT
TTATCTGAAAGTTACTTTGTGAATCCAGAAAACTTTGAGGATGTCACTAATAGTGTGAATATTACATTCC
CAAATCCAAATCAATATAAAGTAGAGTTTAATACGCCTGATGATCAAATTACAACACCGTATATAGTAGT
TGTTAATGGTCATATTGATCCGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAAC
TCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTG
ACGGTATCGATAAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGA
TTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGT
GATTCTACATCAGATAGTGGTTCAGATTCAGCGAGTGATTCAGATTCAGCAAGTGATTCAGACTCAGCGA
GTGATTCAGATTCAGCAAGCGATTCCGACTCAGCGAGCGATTCCGACTCAGACAATGACTCGGATTCAGA
TAGCGATTCTGACTCAGACAGTGACTCAGATTCCGACAGTGACTCAGATTCAGATAGCGATTCTGACTCA
GACAGTGACTCAGATTCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCCGACAGTGATTCCGACT
CAGACAGCGATTCTGACTCCGACAGTGATTCCGACTCAGACAGCGATTCAGATTCCGACAGTGATTCCGA
CTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCA
GATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATT
CAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCAGACTCAGACAGTGATTCGGATTCAGCGAGTGA
TTCGGATTCAGATAGTGATTCCGACTCCGACAGTGACTCGGATTCAGATAGCGACTCAGACTCGGATAGC
GACTCGGATTCAGATAGCGATTCGGACTCAGATAGCGATTCAGAATCAGACAGCGATTCAGAATCAGACA
GCGATTCAGATTCAGACAGCGACTCAGACAGTGACTCAGATTCAGATAGTGACTCGGATTCAGCGAGTGA
TTCAGACTCAGGTAGTGACTCCGATTCATCAAGTGATTCCGACTCAGAAAGTGATTCAAATAGCGATTCC
GAGTCAGGTTCTAACAATAATGTAGTTCCGCCTAATTCACCTAAAAATGGTACTAATGCTTCTAATAAAA
ATGAGGCTAAAGATAGTAAAGAACCATTACCAGATACAGGTTCTGAAGATGAAGCAAATACGTCACTAAT
TTGGGGATTATTAGCATCAATAGGTTCATTACTACTTTTCAGAAGAAAAAAAGAAAATAAAGATAAGAAA
TA

Protein Information

Protein Name

clumping factor A

NCBI Protein GI

151220968

Protein Accession

YP_001331790

3D structure: PDB ID

1N67

Protein pI

3.44

Protein Weight

91994.79

Protein Length

933

Protein Note

fibrinogen-binding protein A

Protein Sequence

>YP_001331790.1 clumping factor A [Staphylococcus aureus subsp. aureus str. Newman]
MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSVTQSDSASNESKSNDSSSVSAAPKTDD
TNVSDTKTSSNTNNGETSVAQNPAQQETTQSSSTNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEE
LVNQTSNETTFNDTNTVSSVNSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSA
PRMRAFSLAAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVP
KELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLTMPAYIDPENVKKTGNV
TLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVIAPVLTGNLKPNT
DSNALIDQQNTSIKVYKVDNAADLSESYFVNPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVV
VNGHIDPNSKGDLALRSTLYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPED
SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSDNDSDSD
SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASD
SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASD
SDSGSDSDSSSDSDSESDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLI
WGLLASIGSLLLFRRKKENKDKK

Epitope Information

IEDB Linear Epitope