Researchers have developed DNA vaccines expressing M. tuberculosis antigen 85A (Ag85A, fbpA) and cytokine granulocyte macrophage colony stimulating factor (GM-CSF). The immune strategy of BCG-prime and DNA vaccine boost induced mice to generate efficient immune protection against M. tuberculosis challenge (Dou et al., 2010).
COG
COG0627R, under R: General function prediction only
Dou et al., 2010: Dou J, Tang Q, Yu F, Yang H, Zhao F, Xu W, Wang J, Hu W, Hu K, Liou C, Feng He X, Wang Y. Investigation of immunogenic effect of the BCG priming and Ag85A- GM-CSF boosting in Balb/c mice model. Immunobiology. 2010; 215(2); 133-142. [PubMed: 19450898].
Rv3804c, (MT3911, MTV026.09c), len: 338 aa. fbpA (alternate gene names: mpt44, 85A), precursor of the 85-A antigen (fibronectin-binding protein A) (mycolyl transferase 85A) (EC 2.3.1.-) (see citations below), identical to P17944|P17996|FBPA|MPT44 ANTIGEN 85-A PRECURSOR from Mycobacterium bovis (338 aa), FASTA scores: opt: 2341, E(): 1.2e-132, (100.0% identity in 338 aa overlap); and highly similar to other Mycobacterial antigen precursors e.g. O52956|A85A_MYCAV|FBPA ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium avium (347 aa), FASTA scores: opt: 1987, E(): 1.7e-111, (82.55% identity in 338 aa overlap); Q05861|A85A_MYCLE|FBPA|ML0097 ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium leprae (330 aa), FASTA scores: opt: 1936, E(): 1.9e-108, (83.0% identity in 329 aa overlap); O06052|A85A_MYCGO|FBPA ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium gordonae (339 aa), FASTA scores: opt: 1932, E(): 3.3e-108, (80.45% identity in 338 aa overlap); etc. Also highly similar to P31952|A85B_MYCTU|FBPB|Rv1886c|MT1934|MTCY180.32 SECRETED ANTIGEN 85-B from Mycobacterium tuberculosis (325 aa), FASTA scores: opt: 1830, E(): 3.9e-102, (78.85% identity in 317 aa overlap); P31953|A85C_MYCTU|FBPC|MPT45|Rv0129c|MTCI5.03c|FBPC2 SECRETED ANTIGEN 85-C from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 1597, E(): 3.4e-88, (67.25% identity in 336 aa overlap).; mpt44; 85A
Protein Annotation
FUNCTION: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity (UniProt: P0A4V2)
SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A4V2)
PTM: Contains one disulfide bridge (UniProt: P0A4V2)
SIMILARITY: Belongs to the mycobacterial A85 antigen family (UniProt: P0A4V2)
