VIOLIN: Vaccine Investigation and Online Information Network

MPT83

General Information

Protegen ID

867

Sequence Strain (Species/Organism)

Mycobacterium tuberculosis H37Rv

VO ID

VO_0012409

Taxonomy ID

83332

Molecule Role

Protective antigen

Molecule Role Annotation

A single DNA vaccine encoding the MPT-83 tuberculosis antigens evoked partial protection against an aerogenic challenge with M. tuberculosis Erdman in the mouse model of pulmonary tuberculosis (Morris et al., 2000).

Related Vaccines(s)

M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs) , M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83

References

Morris et al., 2000: Morris S, Kelley C, Howard A, Li Z, Collins F. The immunogenicity of single and combination DNA vaccines against tuberculosis. Vaccine. 2000; 18(20); 2155-2163. [PubMed: 10715531].

Gene Information

Gene Name

MPT83

NCBI Gene ID

887155

Genbank Accession

AL123456

Locus Tag

Rv2873

Gene Starting Position

3183904

Gene Ending Position

3184566

DNA Sequence

>NC_000962.3:3183904-3184566 Mycobacterium tuberculosis H37Rv, complete genome
GATGATCAACGTTCAGGCCAAACCGGCCGCAGCAGCGAGCCTCGCAGCCATCGCGATTGCGTTCTTAGCG
GGTTGTTCGAGCACCAAACCCGTGTCGCAAGACACCAGCCCGAAACCGGCGACCAGCCCGGCGGCGCCCG
TTACCACGGCGGCAATGGCTGACCCCGCAGCGGACCTGATTGGTCGTGGGTGCGCGCAATACGCGGCGCA
AAATCCCACCGGTCCCGGATCGGTGGCCGGAATGGCGCAAGACCCGGTCGCTACCGCGGCTTCCAACAAC
CCGATGCTCAGTACCCTGACCTCGGCTCTGTCGGGCAAGCTGAACCCGGATGTGAATCTGGTCGACACCC
TCAACGGCGGCGAGTACACCGTTTTCGCCCCCACCAACGCCGCATTCGACAAGCTGCCGGCGGCCACTAT
CGATCAACTCAAGACTGACGCCAAGCTGCTCAGCAGCATCCTGACCTACCACGTGATAGCCGGCCAGGCG
AGTCCGAGCAGGATCGACGGCACCCATCAGACCCTGCAAGGTGCCGACCTGACGGTGATAGGCGCCCGCG
ACGACCTCATGGTCAACAACGCCGGTTTGGTATGTGGCGGAGTTCACACCGCCAACGCGACGGTGTACAT
GATCGATACGGTGCTGATGCCCCCGGCACAGTA

Protein Information

Protein Name

cell surface lipoprotein

NCBI Protein GI

15610010

Protein Accession

NP_217389

Protein pI

4.73

Protein Weight

20581.44

Protein Length

220

Protein Note

Rv2873, (MTCY274.04), len: 220 aa. mpt83 (alternate gene name: mpb83), cell surface lipoprotein (see citations below). Also similar to upstream ORF Q50769|MP70_MYCTU|MPT70|MPB70|Rv2875|MT2943|MTCY274.06 which is also known as MAJOR SECRETED IMMUNOGENIC PROTEIN MPT70 PRECURSOR from Mycobacterium tuberculosis (193 aa), FASTA scores: opt: 806, E(): 2.7e-38, (70.25% identity in 185 aa overlap). BELONGS TO THE MPT70 / MPT83 FAMILY. ATTACHED TO THE MEMBRANE BY A LIPID ANCHOR.; mpb83

Protein Sequence

>NP_217389.1 cell surface lipoprotein [Mycobacterium tuberculosis H37Rv]
MINVQAKPAAAASLAAIAIAFLAGCSSTKPVSQDTSPKPATSPAAPVTTAAMADPAADLIGRGCAQYAAQ
NPTGPGSVAGMAQDPVATAASNNPMLSTLTSALSGKLNPDVNLVDTLNGGEYTVFAPTNAAFDKLPAATI
DQLKTDAKLLSSILTYHVIAGQASPSRIDGTHQTLQGADLTVIGARDDLMVNNAGLVCGGVHTANATVYM
IDTVLMPPAQ

Epitope Information

IEDB Linear Epitope